Rheology of carboxymethyl cellulose solutions treated with cellulases
The effect of cellulase treatments on the rheology of carboxymethyl cellulose (CMC) solutions was studied using a rotational viscometer. The rheological behaviors of CMC solutions of different molecular mass and degrees of substitution where studied as a function of time after various treatments....
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| Format: | Article |
| Language: | English |
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North Carolina State University
2007-02-01
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| Series: | BioResources |
| Subjects: | |
| Online Access: | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/viewFile/BioRes_02_1_020_033_Rheol_CMC_Solutions_Cellulases/30 |
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| author | Lee, J. M. Heitmann, J. A. Pawlak, J. J. |
| author_facet | Lee, J. M. Heitmann, J. A. Pawlak, J. J. |
| author_sort | Lee, J. M. |
| collection | DOAJ |
| description | The effect of cellulase treatments on the rheology of carboxymethyl cellulose (CMC) solutions was studied using a rotational viscometer. The rheological behaviors of CMC solutions of different molecular mass and degrees of substitution where studied as a function of time after various treatments. These solutions were subjected to active and heat-denatured cellulase, a cationic polyelectrolyte (C-PAM), as well as different shear rates. A complex protein-polymer interaction was observed, leading to a potential error source in the measurement of enzymatic activity by changes in the intrinsic viscosity. The interaction was termed a polymeric effect and defined as a reduction in viscosity of the substrate solution without significant formation of reducing sugars from enzymatic hydrolysis. The cause of the reduction in viscosity appears to be related to the interaction between the enzymes as amphipathic particles and the soluble CMC. Thus, the polymeric effect may cause a considerable experimental error in the measurement of enzymatic activity by viscometric methods. |
| first_indexed | 2024-04-12T14:49:44Z |
| format | Article |
| id | doaj.art-61b7915b62574f0f8ad1a45598e60bd5 |
| institution | Directory Open Access Journal |
| issn | 1930-2126 |
| language | English |
| last_indexed | 2024-04-12T14:49:44Z |
| publishDate | 2007-02-01 |
| publisher | North Carolina State University |
| record_format | Article |
| series | BioResources |
| spelling | doaj.art-61b7915b62574f0f8ad1a45598e60bd52022-12-22T03:28:29ZengNorth Carolina State UniversityBioResources1930-21262007-02-01212033Rheology of carboxymethyl cellulose solutions treated with cellulasesLee, J. M.Heitmann, J. A.Pawlak, J. J.The effect of cellulase treatments on the rheology of carboxymethyl cellulose (CMC) solutions was studied using a rotational viscometer. The rheological behaviors of CMC solutions of different molecular mass and degrees of substitution where studied as a function of time after various treatments. These solutions were subjected to active and heat-denatured cellulase, a cationic polyelectrolyte (C-PAM), as well as different shear rates. A complex protein-polymer interaction was observed, leading to a potential error source in the measurement of enzymatic activity by changes in the intrinsic viscosity. The interaction was termed a polymeric effect and defined as a reduction in viscosity of the substrate solution without significant formation of reducing sugars from enzymatic hydrolysis. The cause of the reduction in viscosity appears to be related to the interaction between the enzymes as amphipathic particles and the soluble CMC. Thus, the polymeric effect may cause a considerable experimental error in the measurement of enzymatic activity by viscometric methods.http://ojs.cnr.ncsu.edu/index.php/BioRes/article/viewFile/BioRes_02_1_020_033_Rheol_CMC_Solutions_Cellulases/30Carboxymethyl cellulose (CMC)Endoglucanase activityCellulaseViscosityRotational viscometerThermal denaturationPolymeric effects |
| spellingShingle | Lee, J. M. Heitmann, J. A. Pawlak, J. J. Rheology of carboxymethyl cellulose solutions treated with cellulases BioResources Carboxymethyl cellulose (CMC) Endoglucanase activity Cellulase Viscosity Rotational viscometer Thermal denaturation Polymeric effects |
| title | Rheology of carboxymethyl cellulose solutions treated with cellulases |
| title_full | Rheology of carboxymethyl cellulose solutions treated with cellulases |
| title_fullStr | Rheology of carboxymethyl cellulose solutions treated with cellulases |
| title_full_unstemmed | Rheology of carboxymethyl cellulose solutions treated with cellulases |
| title_short | Rheology of carboxymethyl cellulose solutions treated with cellulases |
| title_sort | rheology of carboxymethyl cellulose solutions treated with cellulases |
| topic | Carboxymethyl cellulose (CMC) Endoglucanase activity Cellulase Viscosity Rotational viscometer Thermal denaturation Polymeric effects |
| url | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/viewFile/BioRes_02_1_020_033_Rheol_CMC_Solutions_Cellulases/30 |
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