A Metagenomic Investigation of the Duodenal Microbiota Reveals Links with Obesity.
Few studies have tested the small intestine microbiota in humans, where most nutrient digestion and absorption occur. Here, our objective was to examine the duodenal microbiota between obese and normal volunteers using metagenomic techniques.We tested duodenal samples from five obese and five normal...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4565581?pdf=render |
| _version_ | 1828840781838811136 |
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| author | Emmanouil Angelakis Fabrice Armougom Frédéric Carrière Dipankar Bachar René Laugier Jean-Christophe Lagier Catherine Robert Caroline Michelle Bernard Henrissat Didier Raoult |
| author_facet | Emmanouil Angelakis Fabrice Armougom Frédéric Carrière Dipankar Bachar René Laugier Jean-Christophe Lagier Catherine Robert Caroline Michelle Bernard Henrissat Didier Raoult |
| author_sort | Emmanouil Angelakis |
| collection | DOAJ |
| description | Few studies have tested the small intestine microbiota in humans, where most nutrient digestion and absorption occur. Here, our objective was to examine the duodenal microbiota between obese and normal volunteers using metagenomic techniques.We tested duodenal samples from five obese and five normal volunteers using 16S rDNA V6 pyrosequencing and Illumina MiSeq deep sequencing. The predominant phyla of the duodenal microbiota were Firmicutes and Actinobacteria, whereas Bacteroidetes were absent. Obese individuals had a significant increase in anaerobic genera (p < 0.001) and a higher abundance of genes encoding Acyl-CoA dehydrogenase (p = 0.0018) compared to the control group. Obese individuals also had a reduced abundance of genes encoding sucrose phosphorylase (p = 0.015) and 1,4-alpha-glucan branching enzyme (p = 0.05). Normal weight people had significantly increased FabK (p = 0.027), and the glycerophospholipid metabolism pathway revealed the presence of phospholipase A1 only in the control group (p = 0.05).The duodenal microbiota of obese individuals exhibit alterations in the fatty acid and sucrose breakdown pathways, probably induced by diet imbalance. |
| first_indexed | 2024-12-12T19:49:24Z |
| format | Article |
| id | doaj.art-61ba906ea24d4d768bdd76527875212e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T19:49:24Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-61ba906ea24d4d768bdd76527875212e2022-12-22T00:14:01ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01109e013778410.1371/journal.pone.0137784A Metagenomic Investigation of the Duodenal Microbiota Reveals Links with Obesity.Emmanouil AngelakisFabrice ArmougomFrédéric CarrièreDipankar BacharRené LaugierJean-Christophe LagierCatherine RobertCaroline MichelleBernard HenrissatDidier RaoultFew studies have tested the small intestine microbiota in humans, where most nutrient digestion and absorption occur. Here, our objective was to examine the duodenal microbiota between obese and normal volunteers using metagenomic techniques.We tested duodenal samples from five obese and five normal volunteers using 16S rDNA V6 pyrosequencing and Illumina MiSeq deep sequencing. The predominant phyla of the duodenal microbiota were Firmicutes and Actinobacteria, whereas Bacteroidetes were absent. Obese individuals had a significant increase in anaerobic genera (p < 0.001) and a higher abundance of genes encoding Acyl-CoA dehydrogenase (p = 0.0018) compared to the control group. Obese individuals also had a reduced abundance of genes encoding sucrose phosphorylase (p = 0.015) and 1,4-alpha-glucan branching enzyme (p = 0.05). Normal weight people had significantly increased FabK (p = 0.027), and the glycerophospholipid metabolism pathway revealed the presence of phospholipase A1 only in the control group (p = 0.05).The duodenal microbiota of obese individuals exhibit alterations in the fatty acid and sucrose breakdown pathways, probably induced by diet imbalance.http://europepmc.org/articles/PMC4565581?pdf=render |
| spellingShingle | Emmanouil Angelakis Fabrice Armougom Frédéric Carrière Dipankar Bachar René Laugier Jean-Christophe Lagier Catherine Robert Caroline Michelle Bernard Henrissat Didier Raoult A Metagenomic Investigation of the Duodenal Microbiota Reveals Links with Obesity. PLoS ONE |
| title | A Metagenomic Investigation of the Duodenal Microbiota Reveals Links with Obesity. |
| title_full | A Metagenomic Investigation of the Duodenal Microbiota Reveals Links with Obesity. |
| title_fullStr | A Metagenomic Investigation of the Duodenal Microbiota Reveals Links with Obesity. |
| title_full_unstemmed | A Metagenomic Investigation of the Duodenal Microbiota Reveals Links with Obesity. |
| title_short | A Metagenomic Investigation of the Duodenal Microbiota Reveals Links with Obesity. |
| title_sort | metagenomic investigation of the duodenal microbiota reveals links with obesity |
| url | http://europepmc.org/articles/PMC4565581?pdf=render |
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