Basic Characterization of Natural Transformation in a Highly Transformable Haemophilus parasuis Strain SC1401
Haemophilus parasuis causes Glässer's disease and pneumonia, incurring serious economic losses in the porcine industry. In this study, natural competence was investigated in H. parasuis. We found competence genes in H. parasuis homologous to ones in Haemophilus influenzae and a high consensus b...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-02-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fcimb.2018.00032/full |
| _version_ | 1811259598486634496 |
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| author | Ke Dai Ke Dai Lvqin He Lvqin He Yung-Fu Chang Sanjie Cao Sanjie Cao Sanjie Cao Qin Zhao Qin Zhao Xiaobo Huang Xiaobo Huang Rui Wu Rui Wu Yong Huang Qigui Yan Xinfeng Han Xiaoping Ma Xintian Wen Xintian Wen Yiping Wen Yiping Wen |
| author_facet | Ke Dai Ke Dai Lvqin He Lvqin He Yung-Fu Chang Sanjie Cao Sanjie Cao Sanjie Cao Qin Zhao Qin Zhao Xiaobo Huang Xiaobo Huang Rui Wu Rui Wu Yong Huang Qigui Yan Xinfeng Han Xiaoping Ma Xintian Wen Xintian Wen Yiping Wen Yiping Wen |
| author_sort | Ke Dai |
| collection | DOAJ |
| description | Haemophilus parasuis causes Glässer's disease and pneumonia, incurring serious economic losses in the porcine industry. In this study, natural competence was investigated in H. parasuis. We found competence genes in H. parasuis homologous to ones in Haemophilus influenzae and a high consensus battery of Sxy-dependent cyclic AMP (cAMP) receptor protein (CRP-S) regulons using bioinformatics. High rates of natural competence were found from the onset of stationary-phase growth condition to mid-stationary phase (OD600 from 0.29 to 1.735); this rapidly dropped off as cells reached mid-stationary phase (OD600 from 1.735 to 1.625). As a whole, bacteria cultured in liquid media were observed to have lower competence levels than those grown on solid media plates. We also revealed that natural transformation in this species is stable after 200 passages and is largely dependent on DNA concentration. Transformation competition experiments showed that heterogeneous DNA cannot outcompete intraspecific natural transformation, suggesting an endogenous uptake sequence or other molecular markers may be important in differentiating heterogeneous DNA. We performed qRT-PCR targeting multiple putative competence genes in an effort to compare bacteria pre-cultured in TSB++ vs. TSA++ and SC1401 vs. SH0165 to determine expression profiles of the homologs of competence-genes in H. influenzae. Taken together, this study is the first to investigate natural transformation in H. parasuis based on a highly naturally transformable strain SC1401. |
| first_indexed | 2024-04-12T18:34:05Z |
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| id | doaj.art-61baac856db04d60acef7908e45255f3 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-04-12T18:34:05Z |
| publishDate | 2018-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-61baac856db04d60acef7908e45255f32022-12-22T03:21:00ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882018-02-01810.3389/fcimb.2018.00032322495Basic Characterization of Natural Transformation in a Highly Transformable Haemophilus parasuis Strain SC1401Ke Dai0Ke Dai1Lvqin He2Lvqin He3Yung-Fu Chang4Sanjie Cao5Sanjie Cao6Sanjie Cao7Qin Zhao8Qin Zhao9Xiaobo Huang10Xiaobo Huang11Rui Wu12Rui Wu13Yong Huang14Qigui Yan15Xinfeng Han16Xiaoping Ma17Xintian Wen18Xintian Wen19Yiping Wen20Yiping Wen21Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaResearch Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaDepartment of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, United StatesResearch Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaSichuan Science-Observation Experimental Station of Veterinary Drugs and Veterinary Diagnostic Technology, Ministry of Agriculture, Chengdu, ChinaResearch Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaResearch Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaResearch Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaResearch Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaResearch Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaHaemophilus parasuis causes Glässer's disease and pneumonia, incurring serious economic losses in the porcine industry. In this study, natural competence was investigated in H. parasuis. We found competence genes in H. parasuis homologous to ones in Haemophilus influenzae and a high consensus battery of Sxy-dependent cyclic AMP (cAMP) receptor protein (CRP-S) regulons using bioinformatics. High rates of natural competence were found from the onset of stationary-phase growth condition to mid-stationary phase (OD600 from 0.29 to 1.735); this rapidly dropped off as cells reached mid-stationary phase (OD600 from 1.735 to 1.625). As a whole, bacteria cultured in liquid media were observed to have lower competence levels than those grown on solid media plates. We also revealed that natural transformation in this species is stable after 200 passages and is largely dependent on DNA concentration. Transformation competition experiments showed that heterogeneous DNA cannot outcompete intraspecific natural transformation, suggesting an endogenous uptake sequence or other molecular markers may be important in differentiating heterogeneous DNA. We performed qRT-PCR targeting multiple putative competence genes in an effort to compare bacteria pre-cultured in TSB++ vs. TSA++ and SC1401 vs. SH0165 to determine expression profiles of the homologs of competence-genes in H. influenzae. Taken together, this study is the first to investigate natural transformation in H. parasuis based on a highly naturally transformable strain SC1401.http://journal.frontiersin.org/article/10.3389/fcimb.2018.00032/fullHaemophilus parasuisCRPuptake signal sequencenatural competencetransformation |
| spellingShingle | Ke Dai Ke Dai Lvqin He Lvqin He Yung-Fu Chang Sanjie Cao Sanjie Cao Sanjie Cao Qin Zhao Qin Zhao Xiaobo Huang Xiaobo Huang Rui Wu Rui Wu Yong Huang Qigui Yan Xinfeng Han Xiaoping Ma Xintian Wen Xintian Wen Yiping Wen Yiping Wen Basic Characterization of Natural Transformation in a Highly Transformable Haemophilus parasuis Strain SC1401 Frontiers in Cellular and Infection Microbiology Haemophilus parasuis CRP uptake signal sequence natural competence transformation |
| title | Basic Characterization of Natural Transformation in a Highly Transformable Haemophilus parasuis Strain SC1401 |
| title_full | Basic Characterization of Natural Transformation in a Highly Transformable Haemophilus parasuis Strain SC1401 |
| title_fullStr | Basic Characterization of Natural Transformation in a Highly Transformable Haemophilus parasuis Strain SC1401 |
| title_full_unstemmed | Basic Characterization of Natural Transformation in a Highly Transformable Haemophilus parasuis Strain SC1401 |
| title_short | Basic Characterization of Natural Transformation in a Highly Transformable Haemophilus parasuis Strain SC1401 |
| title_sort | basic characterization of natural transformation in a highly transformable haemophilus parasuis strain sc1401 |
| topic | Haemophilus parasuis CRP uptake signal sequence natural competence transformation |
| url | http://journal.frontiersin.org/article/10.3389/fcimb.2018.00032/full |
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