Exploring the effects of the dietary fiber compound mediated by a longevity dietary pattern on antioxidation, characteristic bacterial genera, and metabolites based on fecal metabolomics
Abstract Background Age-related dysbiosis of the microbiota has been linked to various negative health outcomes. This study aims to investigate the effects of a newly discovered dietary fiber compound (DFC) on aging, intestinal microbiota, and related metabolic processes. The DFC was identified thro...
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| Format: | Article |
| Language: | English |
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BMC
2024-04-01
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| Series: | Nutrition & Metabolism |
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| Online Access: | https://doi.org/10.1186/s12986-024-00787-y |
| _version_ | 1797220000325435392 |
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| author | Fengcui Shi Qingli Liu Dayong Yue Yanan Zhang Xueying Wei Ying Wang WenJian Ma |
| author_facet | Fengcui Shi Qingli Liu Dayong Yue Yanan Zhang Xueying Wei Ying Wang WenJian Ma |
| author_sort | Fengcui Shi |
| collection | DOAJ |
| description | Abstract Background Age-related dysbiosis of the microbiota has been linked to various negative health outcomes. This study aims to investigate the effects of a newly discovered dietary fiber compound (DFC) on aging, intestinal microbiota, and related metabolic processes. The DFC was identified through in vitro fermentation screening experiments, and its dosage and composition were determined based on a longevity dietary pattern. Methods Aged SPF C57BL/6 J mice (65 weeks old) and young mice (8 weeks old) were divided into three groups: a subgroup without dietary fiber (NDF), a low DFC dose subgroup (LDF, 10% DFC), and a high DFC dose subgroup (HDF, 20% DFC). The total antioxidant capacity (T-AOC), total superoxide dismutase (T-SOD) activity, malondialdehyde (MDA) content, and glutathione peroxidase (GSH-Px) activity in liver and serum samples of the mice were measured according to the manufacturer’s protocol. The expression levels of characteristic bacterial genera and fecal metabolite concentrations in mice were determined using quantitative real-time PCR (qPCR) and nuclear magnetic resonance hydrogen spectroscopy (1H NMR). Metabolomics analysis was further conducted to identify biological functions and potential pathways related to aging. Results After an 8-weeks dietary intervention, DFC supplementation significantly attenuated age-related weight loss, organ degeneration, and oxidative stress. And promoted the growth of Lactobacillus and Bifidobacterium and inhibited the growth of Escherichia coli (E. coli) and Bacteroides (p < 0.05) in the intestinal tracts of aged mice. Metabolomic analysis identified glycolipid and amino acid metabolic pathway biomarkers associated with aging that were differentially regulated by DFC consumption. Correlation analysis between the identified microbial flora and the biomarkers revealed potential mechanistic links between altered microbial composition and metabolic activity with aging markers. Conclusions In conclusion, this study revealed an important mechanism by which DFC consumption impacts healthspan and longevity, shedding light on optimizing dietary fiber or developing fiber-based interventions to improve human health. |
| first_indexed | 2024-04-24T12:42:35Z |
| format | Article |
| id | doaj.art-61bad066e4054c0e8f3a692dafe631c7 |
| institution | Directory Open Access Journal |
| issn | 1743-7075 |
| language | English |
| last_indexed | 2024-04-24T12:42:35Z |
| publishDate | 2024-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Nutrition & Metabolism |
| spelling | doaj.art-61bad066e4054c0e8f3a692dafe631c72024-04-07T11:10:47ZengBMCNutrition & Metabolism1743-70752024-04-0121111410.1186/s12986-024-00787-yExploring the effects of the dietary fiber compound mediated by a longevity dietary pattern on antioxidation, characteristic bacterial genera, and metabolites based on fecal metabolomicsFengcui Shi0Qingli Liu1Dayong Yue2Yanan Zhang3Xueying Wei4Ying Wang5WenJian Ma6School of Chemical and Biological Engineering, Qilu Institute of TechnologySchool of Chemical and Biological Engineering, Qilu Institute of TechnologySchool of Chemical and Biological Engineering, Qilu Institute of TechnologySchool of Chemical and Biological Engineering, Qilu Institute of TechnologySchool of Chemical and Biological Engineering, Qilu Institute of TechnologySchool of Chemical and Biological Engineering, Qilu Institute of TechnologySchool of Chemical and Biological Engineering, Qilu Institute of TechnologyAbstract Background Age-related dysbiosis of the microbiota has been linked to various negative health outcomes. This study aims to investigate the effects of a newly discovered dietary fiber compound (DFC) on aging, intestinal microbiota, and related metabolic processes. The DFC was identified through in vitro fermentation screening experiments, and its dosage and composition were determined based on a longevity dietary pattern. Methods Aged SPF C57BL/6 J mice (65 weeks old) and young mice (8 weeks old) were divided into three groups: a subgroup without dietary fiber (NDF), a low DFC dose subgroup (LDF, 10% DFC), and a high DFC dose subgroup (HDF, 20% DFC). The total antioxidant capacity (T-AOC), total superoxide dismutase (T-SOD) activity, malondialdehyde (MDA) content, and glutathione peroxidase (GSH-Px) activity in liver and serum samples of the mice were measured according to the manufacturer’s protocol. The expression levels of characteristic bacterial genera and fecal metabolite concentrations in mice were determined using quantitative real-time PCR (qPCR) and nuclear magnetic resonance hydrogen spectroscopy (1H NMR). Metabolomics analysis was further conducted to identify biological functions and potential pathways related to aging. Results After an 8-weeks dietary intervention, DFC supplementation significantly attenuated age-related weight loss, organ degeneration, and oxidative stress. And promoted the growth of Lactobacillus and Bifidobacterium and inhibited the growth of Escherichia coli (E. coli) and Bacteroides (p < 0.05) in the intestinal tracts of aged mice. Metabolomic analysis identified glycolipid and amino acid metabolic pathway biomarkers associated with aging that were differentially regulated by DFC consumption. Correlation analysis between the identified microbial flora and the biomarkers revealed potential mechanistic links between altered microbial composition and metabolic activity with aging markers. Conclusions In conclusion, this study revealed an important mechanism by which DFC consumption impacts healthspan and longevity, shedding light on optimizing dietary fiber or developing fiber-based interventions to improve human health.https://doi.org/10.1186/s12986-024-00787-yLongevity dietaryDietary fiber compoundAgingCharacteristic bacterial generaMetabolites |
| spellingShingle | Fengcui Shi Qingli Liu Dayong Yue Yanan Zhang Xueying Wei Ying Wang WenJian Ma Exploring the effects of the dietary fiber compound mediated by a longevity dietary pattern on antioxidation, characteristic bacterial genera, and metabolites based on fecal metabolomics Nutrition & Metabolism Longevity dietary Dietary fiber compound Aging Characteristic bacterial genera Metabolites |
| title | Exploring the effects of the dietary fiber compound mediated by a longevity dietary pattern on antioxidation, characteristic bacterial genera, and metabolites based on fecal metabolomics |
| title_full | Exploring the effects of the dietary fiber compound mediated by a longevity dietary pattern on antioxidation, characteristic bacterial genera, and metabolites based on fecal metabolomics |
| title_fullStr | Exploring the effects of the dietary fiber compound mediated by a longevity dietary pattern on antioxidation, characteristic bacterial genera, and metabolites based on fecal metabolomics |
| title_full_unstemmed | Exploring the effects of the dietary fiber compound mediated by a longevity dietary pattern on antioxidation, characteristic bacterial genera, and metabolites based on fecal metabolomics |
| title_short | Exploring the effects of the dietary fiber compound mediated by a longevity dietary pattern on antioxidation, characteristic bacterial genera, and metabolites based on fecal metabolomics |
| title_sort | exploring the effects of the dietary fiber compound mediated by a longevity dietary pattern on antioxidation characteristic bacterial genera and metabolites based on fecal metabolomics |
| topic | Longevity dietary Dietary fiber compound Aging Characteristic bacterial genera Metabolites |
| url | https://doi.org/10.1186/s12986-024-00787-y |
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