Proinflammatory cytokines and lipopolysaccharides up regulate MMP-3 and MMP-13 production in Asian elephant (Elephas maximus) chondrocytes: attenuation by anti-arthritic agents
Abstract Background Osteoarthritis (OA), the most common form of arthritic disease, results from destruction of joint cartilage and underlying bone. It affects animals, including Asian elephants (Elephas maximus) in captivity, leading to joint pain and lameness. However, publications regarding OA pa...
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| Format: | Article |
| Language: | English |
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BMC
2019-11-01
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| Series: | BMC Veterinary Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12917-019-2170-8 |
| _version_ | 1818530244843274240 |
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| author | Nutnicha Sirikaew Siriwadee Chomdej Siriwan Tangyuenyong Weerapongse Tangjitjaroen Chaleamchat Somgird Chatchote Thitaram Siriwan Ongchai |
| author_facet | Nutnicha Sirikaew Siriwadee Chomdej Siriwan Tangyuenyong Weerapongse Tangjitjaroen Chaleamchat Somgird Chatchote Thitaram Siriwan Ongchai |
| author_sort | Nutnicha Sirikaew |
| collection | DOAJ |
| description | Abstract Background Osteoarthritis (OA), the most common form of arthritic disease, results from destruction of joint cartilage and underlying bone. It affects animals, including Asian elephants (Elephas maximus) in captivity, leading to joint pain and lameness. However, publications regarding OA pathogenesis in this animal are still limited. Therefore, this study aimed to investigate the effect of proinflammatory cytokines, including interleukin-1 beta (IL-1β), IL-17A, tumor necrosis factor-alpha (TNF-α), and oncostatin M (OSM), known mediators of OA pathogenesis, and lipopolysaccharides on the expression of cartilaginous degrading enzymes, matrix metalloproteinase (MMP)-3 and MMP-13, in elephant articular chondrocytes (ELACs) cultures. Anti-arthritic drugs and the active compounds of herbal plants were tested for their potential attenuation against overproduction of these enzymes. Results Among the used cytokines, OSM showed the highest activation of MMP3 and MMP13 expression, especially when combined with IL-1β. The combination of IL-1β and OSM was found to activate phosphorylation of the mitogen-activated protein kinase (MAPK) pathway in ELACs. Lipopolysaccharides or cytokine-induced expressions were suppressed by pharmacologic agents used to treat OA, including dexamethasone, indomethacin, etoricoxib, and diacerein, and by three natural compounds, sesamin, andrographolide, and vanillylacetone. Conclusions Our results revealed the cellular mechanisms underlying OA in elephant chondrocytes, which is triggered by proinflammatory cytokines or lipopolysaccharides and suppressed by common pharmacological or natural medications used to treat human OA. These results provide a more basic understanding of the pathogenesis of elephant OA, which could be useful for adequate medical treatment of OA in this animal. |
| first_indexed | 2024-12-11T17:17:17Z |
| format | Article |
| id | doaj.art-61bf9676b099441a9ef64a7a3e0f24fe |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-12-11T17:17:17Z |
| publishDate | 2019-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-61bf9676b099441a9ef64a7a3e0f24fe2022-12-22T00:57:18ZengBMCBMC Veterinary Research1746-61482019-11-0115111310.1186/s12917-019-2170-8Proinflammatory cytokines and lipopolysaccharides up regulate MMP-3 and MMP-13 production in Asian elephant (Elephas maximus) chondrocytes: attenuation by anti-arthritic agentsNutnicha Sirikaew0Siriwadee Chomdej1Siriwan Tangyuenyong2Weerapongse Tangjitjaroen3Chaleamchat Somgird4Chatchote Thitaram5Siriwan Ongchai6Thailand Excellence Center for Tissue Engineering and Stem Cells, Department of Biochemistry, Faculty of Medicine, Chiang Mai UniversityDepartment of Biology, Faculty of Science, Chiang Mai UniversityDepartment of Companion Animal and Wildlife Clinic, Faculty of Veterinary Medicine, Chiang Mai UniversityDepartment of Companion Animal and Wildlife Clinic, Faculty of Veterinary Medicine, Chiang Mai UniversityDepartment of Companion Animal and Wildlife Clinic, Faculty of Veterinary Medicine, Chiang Mai UniversityDepartment of Companion Animal and Wildlife Clinic, Faculty of Veterinary Medicine, Chiang Mai UniversityThailand Excellence Center for Tissue Engineering and Stem Cells, Department of Biochemistry, Faculty of Medicine, Chiang Mai UniversityAbstract Background Osteoarthritis (OA), the most common form of arthritic disease, results from destruction of joint cartilage and underlying bone. It affects animals, including Asian elephants (Elephas maximus) in captivity, leading to joint pain and lameness. However, publications regarding OA pathogenesis in this animal are still limited. Therefore, this study aimed to investigate the effect of proinflammatory cytokines, including interleukin-1 beta (IL-1β), IL-17A, tumor necrosis factor-alpha (TNF-α), and oncostatin M (OSM), known mediators of OA pathogenesis, and lipopolysaccharides on the expression of cartilaginous degrading enzymes, matrix metalloproteinase (MMP)-3 and MMP-13, in elephant articular chondrocytes (ELACs) cultures. Anti-arthritic drugs and the active compounds of herbal plants were tested for their potential attenuation against overproduction of these enzymes. Results Among the used cytokines, OSM showed the highest activation of MMP3 and MMP13 expression, especially when combined with IL-1β. The combination of IL-1β and OSM was found to activate phosphorylation of the mitogen-activated protein kinase (MAPK) pathway in ELACs. Lipopolysaccharides or cytokine-induced expressions were suppressed by pharmacologic agents used to treat OA, including dexamethasone, indomethacin, etoricoxib, and diacerein, and by three natural compounds, sesamin, andrographolide, and vanillylacetone. Conclusions Our results revealed the cellular mechanisms underlying OA in elephant chondrocytes, which is triggered by proinflammatory cytokines or lipopolysaccharides and suppressed by common pharmacological or natural medications used to treat human OA. These results provide a more basic understanding of the pathogenesis of elephant OA, which could be useful for adequate medical treatment of OA in this animal.http://link.springer.com/article/10.1186/s12917-019-2170-8Elephas maximusOsteoarthritisProinflammatory cytokinesMMP-3MMP-13 |
| spellingShingle | Nutnicha Sirikaew Siriwadee Chomdej Siriwan Tangyuenyong Weerapongse Tangjitjaroen Chaleamchat Somgird Chatchote Thitaram Siriwan Ongchai Proinflammatory cytokines and lipopolysaccharides up regulate MMP-3 and MMP-13 production in Asian elephant (Elephas maximus) chondrocytes: attenuation by anti-arthritic agents BMC Veterinary Research Elephas maximus Osteoarthritis Proinflammatory cytokines MMP-3 MMP-13 |
| title | Proinflammatory cytokines and lipopolysaccharides up regulate MMP-3 and MMP-13 production in Asian elephant (Elephas maximus) chondrocytes: attenuation by anti-arthritic agents |
| title_full | Proinflammatory cytokines and lipopolysaccharides up regulate MMP-3 and MMP-13 production in Asian elephant (Elephas maximus) chondrocytes: attenuation by anti-arthritic agents |
| title_fullStr | Proinflammatory cytokines and lipopolysaccharides up regulate MMP-3 and MMP-13 production in Asian elephant (Elephas maximus) chondrocytes: attenuation by anti-arthritic agents |
| title_full_unstemmed | Proinflammatory cytokines and lipopolysaccharides up regulate MMP-3 and MMP-13 production in Asian elephant (Elephas maximus) chondrocytes: attenuation by anti-arthritic agents |
| title_short | Proinflammatory cytokines and lipopolysaccharides up regulate MMP-3 and MMP-13 production in Asian elephant (Elephas maximus) chondrocytes: attenuation by anti-arthritic agents |
| title_sort | proinflammatory cytokines and lipopolysaccharides up regulate mmp 3 and mmp 13 production in asian elephant elephas maximus chondrocytes attenuation by anti arthritic agents |
| topic | Elephas maximus Osteoarthritis Proinflammatory cytokines MMP-3 MMP-13 |
| url | http://link.springer.com/article/10.1186/s12917-019-2170-8 |
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