H2O2-Preconditioned Umbilical Cord-Derived Mesenchymal Stem Cells Ameliorate Liver Regeneration in Acute Liver Failure-Induced Mice
Introduction: Mesenchymal stem cells (MSCs) are suitable candidates for the treatment of liver diseases. However, their low survival rate limits their efficacy following transplantation. This study aimed to evaluate the therapeutic potentials of H2O2-preconditioned umbilical cord-derived MSCs (UCMSC...
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| Format: | Article |
| Language: | English |
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Negah Institute for Scientific Communication
2017-01-01
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| Series: | Anatomical Sciences Journal |
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| Online Access: | http://anatomyjournal.ir/article-1-187-en.html |
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| author | Fatemeh Nasiri Behrooz Johari Fatemeh Amiri Mehryar Habibi Roudkenar Sedigheh Molaei Marzie Bahadori Marjan Movahed Abazar Yari Mohammad Ali Jalili |
| author_facet | Fatemeh Nasiri Behrooz Johari Fatemeh Amiri Mehryar Habibi Roudkenar Sedigheh Molaei Marzie Bahadori Marjan Movahed Abazar Yari Mohammad Ali Jalili |
| author_sort | Fatemeh Nasiri |
| collection | DOAJ |
| description | Introduction: Mesenchymal stem cells (MSCs) are suitable candidates for the treatment of liver diseases. However, their low survival rate limits their efficacy following transplantation. This study aimed to evaluate the therapeutic potentials of H2O2-preconditioned umbilical cord-derived MSCs (UCMSCs) on acute liver failure (ALF) in mice.
Methods: UCMSCs were pre-conditioned with different concentrations of H2O2. Cell viability was evaluated by WST-1 (water soluble tetrazolium) assay followed by exposure to lethal doses of H2O2. ALF was induced in NMRI mice using CCl4 and the cells therapy was performed using H2O2-preconditioned and normal UCMSCs. After 24, 48, and 72 hours, regenerative potentials of different UCMSCs groups were evaluated compared to sham group (that receive no MSCs) using biochemical and histological methods.
Results: Lower liver enzymes was significantly evident in mice transplanted with H2O2-preconditioned UCMSCs compared with the other groups. Interestingly, histological results revealed a significant improvement in liver regeneration in these mice.
Conclusion: Preconditioning of UCMSCs with H2O2 not only enhances their survival but also increases the efficacy of MSCs-based cell therapy in acute liver failure. |
| first_indexed | 2024-12-12T11:02:59Z |
| format | Article |
| id | doaj.art-61c2bdacf2104cabb00491f5681543dd |
| institution | Directory Open Access Journal |
| issn | 2322-3626 2322-3626 |
| language | English |
| last_indexed | 2024-12-12T11:02:59Z |
| publishDate | 2017-01-01 |
| publisher | Negah Institute for Scientific Communication |
| record_format | Article |
| series | Anatomical Sciences Journal |
| spelling | doaj.art-61c2bdacf2104cabb00491f5681543dd2022-12-22T00:26:28ZengNegah Institute for Scientific CommunicationAnatomical Sciences Journal2322-36262322-36262017-01-011414350H2O2-Preconditioned Umbilical Cord-Derived Mesenchymal Stem Cells Ameliorate Liver Regeneration in Acute Liver Failure-Induced MiceFatemeh Nasiri0Behrooz Johari1Fatemeh Amiri2Mehryar Habibi Roudkenar3Sedigheh Molaei4Marzie Bahadori5Marjan Movahed6Abazar Yari7Mohammad Ali Jalili8 Department of Biochemistry, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran. Department of Biotechnology, Pasteur Institute of Iran, Tehran, Iran. Department of Biochemistry, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran. Department of Biochemistry, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran. Department of Biochemistry, School of Medicine, Qom University of Medical Sciences, Qom, Iran. Department of Biochemistry, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran. Department of Biochemistry, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran. Department of Anatomy, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran. Department of Biochemistry, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran. Introduction: Mesenchymal stem cells (MSCs) are suitable candidates for the treatment of liver diseases. However, their low survival rate limits their efficacy following transplantation. This study aimed to evaluate the therapeutic potentials of H2O2-preconditioned umbilical cord-derived MSCs (UCMSCs) on acute liver failure (ALF) in mice. Methods: UCMSCs were pre-conditioned with different concentrations of H2O2. Cell viability was evaluated by WST-1 (water soluble tetrazolium) assay followed by exposure to lethal doses of H2O2. ALF was induced in NMRI mice using CCl4 and the cells therapy was performed using H2O2-preconditioned and normal UCMSCs. After 24, 48, and 72 hours, regenerative potentials of different UCMSCs groups were evaluated compared to sham group (that receive no MSCs) using biochemical and histological methods. Results: Lower liver enzymes was significantly evident in mice transplanted with H2O2-preconditioned UCMSCs compared with the other groups. Interestingly, histological results revealed a significant improvement in liver regeneration in these mice. Conclusion: Preconditioning of UCMSCs with H2O2 not only enhances their survival but also increases the efficacy of MSCs-based cell therapy in acute liver failure.http://anatomyjournal.ir/article-1-187-en.htmlacute liver failurecarbon tetrachloridemesenchymal stem cellsoxidative stresspreconditioning |
| spellingShingle | Fatemeh Nasiri Behrooz Johari Fatemeh Amiri Mehryar Habibi Roudkenar Sedigheh Molaei Marzie Bahadori Marjan Movahed Abazar Yari Mohammad Ali Jalili H2O2-Preconditioned Umbilical Cord-Derived Mesenchymal Stem Cells Ameliorate Liver Regeneration in Acute Liver Failure-Induced Mice Anatomical Sciences Journal acute liver failure carbon tetrachloride mesenchymal stem cells oxidative stress preconditioning |
| title | H2O2-Preconditioned Umbilical Cord-Derived Mesenchymal Stem Cells Ameliorate Liver Regeneration in Acute Liver Failure-Induced Mice |
| title_full | H2O2-Preconditioned Umbilical Cord-Derived Mesenchymal Stem Cells Ameliorate Liver Regeneration in Acute Liver Failure-Induced Mice |
| title_fullStr | H2O2-Preconditioned Umbilical Cord-Derived Mesenchymal Stem Cells Ameliorate Liver Regeneration in Acute Liver Failure-Induced Mice |
| title_full_unstemmed | H2O2-Preconditioned Umbilical Cord-Derived Mesenchymal Stem Cells Ameliorate Liver Regeneration in Acute Liver Failure-Induced Mice |
| title_short | H2O2-Preconditioned Umbilical Cord-Derived Mesenchymal Stem Cells Ameliorate Liver Regeneration in Acute Liver Failure-Induced Mice |
| title_sort | h2o2 preconditioned umbilical cord derived mesenchymal stem cells ameliorate liver regeneration in acute liver failure induced mice |
| topic | acute liver failure carbon tetrachloride mesenchymal stem cells oxidative stress preconditioning |
| url | http://anatomyjournal.ir/article-1-187-en.html |
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