Antioxidants and cardioprotective effects of ethyl acetate fraction of Canavalia rosea leaves in myocardial ischemia-reperfusion injury
Abstract Different degrees in the biological activities of Canavalia rosea had been previously reported . In this study, our group assessed the cardioprotective effects of the ethyl acetate fraction (EAcF) of the Canavalia rosea leaves. Firstly, it was confirmed, by in vitro approach, that the EAcF...
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Academia Brasileira de Ciências
2023-07-01
|
| Series: | Anais da Academia Brasileira de Ciências |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652023000200704&lng=en&tlng=en |
| _version_ | 1797783304467906560 |
|---|---|
| author | MARAÍSA B.J. FEITOSA SILVAN S. ARAÚJO THÁSSIO RICARDO R. MESQUITA CAROLINA R. GIODA LUCAS A. DE SÁ GRACE KELLY M. ALMEIDA RODRIGO MIGUEL-DOS-SANTOS ANDRIELE M. BARBOSA CARLA MARIA L. DE VASCONCELOS ENILTON A. CAMARGO ANDRÉ LUÍS B.S. BARREIROS CHARLES S. ESTEVAM ÉDER RICARDO DE MORAES RICARDO G. AMARAL SANDRA LAUTON-SANTOS |
| author_facet | MARAÍSA B.J. FEITOSA SILVAN S. ARAÚJO THÁSSIO RICARDO R. MESQUITA CAROLINA R. GIODA LUCAS A. DE SÁ GRACE KELLY M. ALMEIDA RODRIGO MIGUEL-DOS-SANTOS ANDRIELE M. BARBOSA CARLA MARIA L. DE VASCONCELOS ENILTON A. CAMARGO ANDRÉ LUÍS B.S. BARREIROS CHARLES S. ESTEVAM ÉDER RICARDO DE MORAES RICARDO G. AMARAL SANDRA LAUTON-SANTOS |
| author_sort | MARAÍSA B.J. FEITOSA |
| collection | DOAJ |
| description | Abstract Different degrees in the biological activities of Canavalia rosea had been previously reported . In this study, our group assessed the cardioprotective effects of the ethyl acetate fraction (EAcF) of the Canavalia rosea leaves. Firstly, it was confirmed, by in vitro approach, that the EAcF has high antioxidant properties due to the presence of important secondary metabolites, as flavonoids. In order to explore their potential protector against cardiovascular disorders, hearts were previously perfused with EAcF (300 μg.mL-1) and submitted to the global ischemia followed by reperfusion in Langendorff system. The present findings have demonstrated that EAcF restored the left ventricular developed pressure and decreased the arrhythmias severity index. Furthermore, EAcF significantly increased the glutathiones peroxidase activity with decreased malondialdehyde and creatine kinase levels. EAcF was effective upon neither the superoxide dismutase, glutationes reductase nor the catalase activities. In addition, the Western blot analysis revealed that ischemia-reperfusion injury significantly upregulates caspase 3 protein expression, while EAcF abolishes this effect. These results provide evidence that the EAcF reestablishes the cardiac contractility and prevents arrhythmias; it is suggested that EAcF could be used to reduce injury caused by cardiac reperfusion. However more clinical studies should be performed, before applying it in the clinic. |
| first_indexed | 2024-03-13T00:24:09Z |
| format | Article |
| id | doaj.art-61c342d6f2404cc3a2452cfbaf8c2308 |
| institution | Directory Open Access Journal |
| issn | 1678-2690 |
| language | English |
| last_indexed | 2024-03-13T00:24:09Z |
| publishDate | 2023-07-01 |
| publisher | Academia Brasileira de Ciências |
| record_format | Article |
| series | Anais da Academia Brasileira de Ciências |
| spelling | doaj.art-61c342d6f2404cc3a2452cfbaf8c23082023-07-11T07:47:54ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902023-07-0195suppl 110.1590/0001-3765202320220514Antioxidants and cardioprotective effects of ethyl acetate fraction of Canavalia rosea leaves in myocardial ischemia-reperfusion injuryMARAÍSA B.J. FEITOSAhttps://orcid.org/0000-0002-2290-1585SILVAN S. ARAÚJOhttps://orcid.org/0000-0003-2469-1304THÁSSIO RICARDO R. MESQUITAhttps://orcid.org/0000-0002-6927-5745CAROLINA R. GIODAhttps://orcid.org/0000-0001-5975-1675LUCAS A. DE SÁhttps://orcid.org/0000-0001-8965-3275GRACE KELLY M. ALMEIDAhttps://orcid.org/0009-0001-3653-523XRODRIGO MIGUEL-DOS-SANTOShttps://orcid.org/0000-0001-8839-3416ANDRIELE M. BARBOSAhttps://orcid.org/0000-0003-3326-4750CARLA MARIA L. DE VASCONCELOShttps://orcid.org/0000-0002-7197-0261ENILTON A. CAMARGOhttps://orcid.org/0000-0003-2745-0768ANDRÉ LUÍS B.S. BARREIROShttps://orcid.org/0000-0002-7263-1907CHARLES S. ESTEVAMhttps://orcid.org/0000-0001-6940-6891ÉDER RICARDO DE MORAEShttps://orcid.org/0000-0002-0977-2328RICARDO G. AMARALhttps://orcid.org/0000-0002-9266-6840SANDRA LAUTON-SANTOShttps://orcid.org/0000-0003-3373-3254Abstract Different degrees in the biological activities of Canavalia rosea had been previously reported . In this study, our group assessed the cardioprotective effects of the ethyl acetate fraction (EAcF) of the Canavalia rosea leaves. Firstly, it was confirmed, by in vitro approach, that the EAcF has high antioxidant properties due to the presence of important secondary metabolites, as flavonoids. In order to explore their potential protector against cardiovascular disorders, hearts were previously perfused with EAcF (300 μg.mL-1) and submitted to the global ischemia followed by reperfusion in Langendorff system. The present findings have demonstrated that EAcF restored the left ventricular developed pressure and decreased the arrhythmias severity index. Furthermore, EAcF significantly increased the glutathiones peroxidase activity with decreased malondialdehyde and creatine kinase levels. EAcF was effective upon neither the superoxide dismutase, glutationes reductase nor the catalase activities. In addition, the Western blot analysis revealed that ischemia-reperfusion injury significantly upregulates caspase 3 protein expression, while EAcF abolishes this effect. These results provide evidence that the EAcF reestablishes the cardiac contractility and prevents arrhythmias; it is suggested that EAcF could be used to reduce injury caused by cardiac reperfusion. However more clinical studies should be performed, before applying it in the clinic.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652023000200704&lng=en&tlng=enenzymesantioxidant effectcardiac contractilityCanavalia rosea |
| spellingShingle | MARAÍSA B.J. FEITOSA SILVAN S. ARAÚJO THÁSSIO RICARDO R. MESQUITA CAROLINA R. GIODA LUCAS A. DE SÁ GRACE KELLY M. ALMEIDA RODRIGO MIGUEL-DOS-SANTOS ANDRIELE M. BARBOSA CARLA MARIA L. DE VASCONCELOS ENILTON A. CAMARGO ANDRÉ LUÍS B.S. BARREIROS CHARLES S. ESTEVAM ÉDER RICARDO DE MORAES RICARDO G. AMARAL SANDRA LAUTON-SANTOS Antioxidants and cardioprotective effects of ethyl acetate fraction of Canavalia rosea leaves in myocardial ischemia-reperfusion injury Anais da Academia Brasileira de Ciências enzymes antioxidant effect cardiac contractility Canavalia rosea |
| title | Antioxidants and cardioprotective effects of ethyl acetate fraction of Canavalia rosea leaves in myocardial ischemia-reperfusion injury |
| title_full | Antioxidants and cardioprotective effects of ethyl acetate fraction of Canavalia rosea leaves in myocardial ischemia-reperfusion injury |
| title_fullStr | Antioxidants and cardioprotective effects of ethyl acetate fraction of Canavalia rosea leaves in myocardial ischemia-reperfusion injury |
| title_full_unstemmed | Antioxidants and cardioprotective effects of ethyl acetate fraction of Canavalia rosea leaves in myocardial ischemia-reperfusion injury |
| title_short | Antioxidants and cardioprotective effects of ethyl acetate fraction of Canavalia rosea leaves in myocardial ischemia-reperfusion injury |
| title_sort | antioxidants and cardioprotective effects of ethyl acetate fraction of canavalia rosea leaves in myocardial ischemia reperfusion injury |
| topic | enzymes antioxidant effect cardiac contractility Canavalia rosea |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652023000200704&lng=en&tlng=en |
| work_keys_str_mv | AT maraisabjfeitosa antioxidantsandcardioprotectiveeffectsofethylacetatefractionofcanavaliarosealeavesinmyocardialischemiareperfusioninjury AT silvansaraujo antioxidantsandcardioprotectiveeffectsofethylacetatefractionofcanavaliarosealeavesinmyocardialischemiareperfusioninjury AT thassioricardormesquita antioxidantsandcardioprotectiveeffectsofethylacetatefractionofcanavaliarosealeavesinmyocardialischemiareperfusioninjury AT carolinargioda antioxidantsandcardioprotectiveeffectsofethylacetatefractionofcanavaliarosealeavesinmyocardialischemiareperfusioninjury AT lucasadesa antioxidantsandcardioprotectiveeffectsofethylacetatefractionofcanavaliarosealeavesinmyocardialischemiareperfusioninjury AT gracekellymalmeida antioxidantsandcardioprotectiveeffectsofethylacetatefractionofcanavaliarosealeavesinmyocardialischemiareperfusioninjury AT rodrigomigueldossantos antioxidantsandcardioprotectiveeffectsofethylacetatefractionofcanavaliarosealeavesinmyocardialischemiareperfusioninjury AT andrielembarbosa antioxidantsandcardioprotectiveeffectsofethylacetatefractionofcanavaliarosealeavesinmyocardialischemiareperfusioninjury AT carlamarialdevasconcelos antioxidantsandcardioprotectiveeffectsofethylacetatefractionofcanavaliarosealeavesinmyocardialischemiareperfusioninjury AT eniltonacamargo antioxidantsandcardioprotectiveeffectsofethylacetatefractionofcanavaliarosealeavesinmyocardialischemiareperfusioninjury AT andreluisbsbarreiros antioxidantsandcardioprotectiveeffectsofethylacetatefractionofcanavaliarosealeavesinmyocardialischemiareperfusioninjury AT charlessestevam antioxidantsandcardioprotectiveeffectsofethylacetatefractionofcanavaliarosealeavesinmyocardialischemiareperfusioninjury AT ederricardodemoraes antioxidantsandcardioprotectiveeffectsofethylacetatefractionofcanavaliarosealeavesinmyocardialischemiareperfusioninjury AT ricardogamaral antioxidantsandcardioprotectiveeffectsofethylacetatefractionofcanavaliarosealeavesinmyocardialischemiareperfusioninjury AT sandralautonsantos antioxidantsandcardioprotectiveeffectsofethylacetatefractionofcanavaliarosealeavesinmyocardialischemiareperfusioninjury |