Screening of Polyvalent Phage-Resistant Escherichia coli Strains Based on Phage Receptor Analysis
Bacteria-based biotechnology processes are constantly under threat from bacteriophage infection, with phage contamination being a non-neglectable problem for microbial fermentation. The essence of this problem is the complex co-evolutionary relationship between phages and bacteria. The development o...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.00850/full |
| _version_ | 1828487408652386304 |
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| author | Ping Li Ping Li Hong Lin Zhiqiang Mi Shaozhen Xing Yigang Tong Yigang Tong Jingxue Wang |
| author_facet | Ping Li Ping Li Hong Lin Zhiqiang Mi Shaozhen Xing Yigang Tong Yigang Tong Jingxue Wang |
| author_sort | Ping Li |
| collection | DOAJ |
| description | Bacteria-based biotechnology processes are constantly under threat from bacteriophage infection, with phage contamination being a non-neglectable problem for microbial fermentation. The essence of this problem is the complex co-evolutionary relationship between phages and bacteria. The development of phage control strategies requires further knowledge about phage-host interactions, while the widespread use of Escherichia coli strain BL21 (DE3) in biotechnological processes makes the study of phage receptors in this strain particularly important. Here, eight phages infecting E. coli BL21 (DE3) via different receptors were isolated and subsequently identified as members of the genera T4virus, Js98virus, Felix01virus, T1virus, and Rtpvirus. Phage receptors were identified by whole-genome sequencing of phage-resistant E. coli strains and sequence comparison with wild-type BL21 (DE3). Results showed that the receptors for the isolated phages, designated vB_EcoS_IME18, vB_EcoS_IME253, vB_EcoM_IME281, vB_EcoM_IME338, vB_EcoM_IME339, vB_EcoM_IME340, vB_EcoM_IME341, and vB_EcoS_IME347 were FhuA, FepA, OmpF, lipopolysaccharide, Tsx, OmpA, FadL, and YncD, respectively. A polyvalent phage-resistant BL21 (DE3)-derived strain, designated PR8, was then identified by screening with a phage cocktail consisting of the eight phages. Strain PR8 is resistant to 23 of 32 tested phages including Myoviridae and Siphoviridae phages. Strains BL21 (DE3) and PR8 showed similar expression levels of enhanced green fluorescent protein. Thus, PR8 may be used as a phage resistant strain for fermentation processes. The findings of this study contribute significantly to our knowledge of phage-host interactions and may help prevent phage contamination in fermentation. |
| first_indexed | 2024-12-11T09:50:03Z |
| format | Article |
| id | doaj.art-61c37eb7cbe14614b7078f4c49e0614e |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-11T09:50:03Z |
| publishDate | 2019-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-61c37eb7cbe14614b7078f4c49e0614e2022-12-22T01:12:26ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-04-011010.3389/fmicb.2019.00850439623Screening of Polyvalent Phage-Resistant Escherichia coli Strains Based on Phage Receptor AnalysisPing Li0Ping Li1Hong Lin2Zhiqiang Mi3Shaozhen Xing4Yigang Tong5Yigang Tong6Jingxue Wang7Food Safety Laboratory, Department of Food Science and Engineering, Ocean University of China, Qingdao, ChinaState Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, ChinaFood Safety Laboratory, Department of Food Science and Engineering, Ocean University of China, Qingdao, ChinaState Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, ChinaState Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, ChinaState Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, ChinaDepartment of Life Science and Technology, Beijing University of Chemical Technology, Beijing, ChinaFood Safety Laboratory, Department of Food Science and Engineering, Ocean University of China, Qingdao, ChinaBacteria-based biotechnology processes are constantly under threat from bacteriophage infection, with phage contamination being a non-neglectable problem for microbial fermentation. The essence of this problem is the complex co-evolutionary relationship between phages and bacteria. The development of phage control strategies requires further knowledge about phage-host interactions, while the widespread use of Escherichia coli strain BL21 (DE3) in biotechnological processes makes the study of phage receptors in this strain particularly important. Here, eight phages infecting E. coli BL21 (DE3) via different receptors were isolated and subsequently identified as members of the genera T4virus, Js98virus, Felix01virus, T1virus, and Rtpvirus. Phage receptors were identified by whole-genome sequencing of phage-resistant E. coli strains and sequence comparison with wild-type BL21 (DE3). Results showed that the receptors for the isolated phages, designated vB_EcoS_IME18, vB_EcoS_IME253, vB_EcoM_IME281, vB_EcoM_IME338, vB_EcoM_IME339, vB_EcoM_IME340, vB_EcoM_IME341, and vB_EcoS_IME347 were FhuA, FepA, OmpF, lipopolysaccharide, Tsx, OmpA, FadL, and YncD, respectively. A polyvalent phage-resistant BL21 (DE3)-derived strain, designated PR8, was then identified by screening with a phage cocktail consisting of the eight phages. Strain PR8 is resistant to 23 of 32 tested phages including Myoviridae and Siphoviridae phages. Strains BL21 (DE3) and PR8 showed similar expression levels of enhanced green fluorescent protein. Thus, PR8 may be used as a phage resistant strain for fermentation processes. The findings of this study contribute significantly to our knowledge of phage-host interactions and may help prevent phage contamination in fermentation.https://www.frontiersin.org/article/10.3389/fmicb.2019.00850/fullEscherichia coli BL21 (DE3)phage contaminationphage-host interactionphage resistancephage receptors |
| spellingShingle | Ping Li Ping Li Hong Lin Zhiqiang Mi Shaozhen Xing Yigang Tong Yigang Tong Jingxue Wang Screening of Polyvalent Phage-Resistant Escherichia coli Strains Based on Phage Receptor Analysis Frontiers in Microbiology Escherichia coli BL21 (DE3) phage contamination phage-host interaction phage resistance phage receptors |
| title | Screening of Polyvalent Phage-Resistant Escherichia coli Strains Based on Phage Receptor Analysis |
| title_full | Screening of Polyvalent Phage-Resistant Escherichia coli Strains Based on Phage Receptor Analysis |
| title_fullStr | Screening of Polyvalent Phage-Resistant Escherichia coli Strains Based on Phage Receptor Analysis |
| title_full_unstemmed | Screening of Polyvalent Phage-Resistant Escherichia coli Strains Based on Phage Receptor Analysis |
| title_short | Screening of Polyvalent Phage-Resistant Escherichia coli Strains Based on Phage Receptor Analysis |
| title_sort | screening of polyvalent phage resistant escherichia coli strains based on phage receptor analysis |
| topic | Escherichia coli BL21 (DE3) phage contamination phage-host interaction phage resistance phage receptors |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.00850/full |
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