Preparation and Functional Assessment of Composite Chitosan-Nano-Hydroxyapatite Scaffolds for Bone Regeneration
Composite chitosan-nano-hydroxyapatite microspheres and scaffolds prepared using a co-precipitation method have shown potential for use in bone regeneration. The goal of this research was to improve the functional properties of the composite scaffolds by modifying the fabrication parameters. The eff...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2012-02-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | http://www.mdpi.com/2079-4983/3/1/114/ |
| _version_ | 1828119055213527040 |
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| author | Warren O. Haggard Joel D. Bumgardner Jessica A. Jennings Benjamin T. Reves |
| author_facet | Warren O. Haggard Joel D. Bumgardner Jessica A. Jennings Benjamin T. Reves |
| author_sort | Warren O. Haggard |
| collection | DOAJ |
| description | Composite chitosan-nano-hydroxyapatite microspheres and scaffolds prepared using a co-precipitation method have shown potential for use in bone regeneration. The goal of this research was to improve the functional properties of the composite scaffolds by modifying the fabrication parameters. The effects of degree of deacetylation (DDA), drying method, hydroxyapatite content and an acid wash on scaffold properties were investigated. Freeze-dried 61% DDA scaffolds degraded faster (3.5 ± 0.5% mass loss) than air-dried 61% DDA scaffolds and 80% DDA scaffolds, but had a lower compressive modulus of 0.12 ± 0.01 MPa. Air-dried 80% DDA scaffolds displayed the highest compressive modulus (3.79 ± 0.51 MPa) and these scaffolds were chosen as the best candidate for use in bone regeneration. Increasing the amount of hydroxyapatite in the air-dried 80% DDA scaffolds did not further increase the compressive modulus of the scaffolds. An acid wash procedure at pH 6.1 was found to increase the degradation of air-dried 80% DDA scaffolds from 1.3 ± 0.1% to 4.4 ± 0.4%. All of the formulations tested supported the proliferation of SAOS-2 cells. |
| first_indexed | 2024-04-11T13:40:35Z |
| format | Article |
| id | doaj.art-61c42ffef2e84f92920b4cd50dc0c16a |
| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-04-11T13:40:35Z |
| publishDate | 2012-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-61c42ffef2e84f92920b4cd50dc0c16a2022-12-22T04:21:14ZengMDPI AGJournal of Functional Biomaterials2079-49832012-02-013111413010.3390/jfb3010114Preparation and Functional Assessment of Composite Chitosan-Nano-Hydroxyapatite Scaffolds for Bone RegenerationWarren O. HaggardJoel D. BumgardnerJessica A. JenningsBenjamin T. RevesComposite chitosan-nano-hydroxyapatite microspheres and scaffolds prepared using a co-precipitation method have shown potential for use in bone regeneration. The goal of this research was to improve the functional properties of the composite scaffolds by modifying the fabrication parameters. The effects of degree of deacetylation (DDA), drying method, hydroxyapatite content and an acid wash on scaffold properties were investigated. Freeze-dried 61% DDA scaffolds degraded faster (3.5 ± 0.5% mass loss) than air-dried 61% DDA scaffolds and 80% DDA scaffolds, but had a lower compressive modulus of 0.12 ± 0.01 MPa. Air-dried 80% DDA scaffolds displayed the highest compressive modulus (3.79 ± 0.51 MPa) and these scaffolds were chosen as the best candidate for use in bone regeneration. Increasing the amount of hydroxyapatite in the air-dried 80% DDA scaffolds did not further increase the compressive modulus of the scaffolds. An acid wash procedure at pH 6.1 was found to increase the degradation of air-dried 80% DDA scaffolds from 1.3 ± 0.1% to 4.4 ± 0.4%. All of the formulations tested supported the proliferation of SAOS-2 cells.http://www.mdpi.com/2079-4983/3/1/114/bone regenerationchitosanhydroxyapatitetissue engineering |
| spellingShingle | Warren O. Haggard Joel D. Bumgardner Jessica A. Jennings Benjamin T. Reves Preparation and Functional Assessment of Composite Chitosan-Nano-Hydroxyapatite Scaffolds for Bone Regeneration Journal of Functional Biomaterials bone regeneration chitosan hydroxyapatite tissue engineering |
| title | Preparation and Functional Assessment of Composite Chitosan-Nano-Hydroxyapatite Scaffolds for Bone Regeneration |
| title_full | Preparation and Functional Assessment of Composite Chitosan-Nano-Hydroxyapatite Scaffolds for Bone Regeneration |
| title_fullStr | Preparation and Functional Assessment of Composite Chitosan-Nano-Hydroxyapatite Scaffolds for Bone Regeneration |
| title_full_unstemmed | Preparation and Functional Assessment of Composite Chitosan-Nano-Hydroxyapatite Scaffolds for Bone Regeneration |
| title_short | Preparation and Functional Assessment of Composite Chitosan-Nano-Hydroxyapatite Scaffolds for Bone Regeneration |
| title_sort | preparation and functional assessment of composite chitosan nano hydroxyapatite scaffolds for bone regeneration |
| topic | bone regeneration chitosan hydroxyapatite tissue engineering |
| url | http://www.mdpi.com/2079-4983/3/1/114/ |
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