No evidence of light inhibition on aerobic methanotrophs in coastal sediments using eDNA and eRNA
Abstract It is estimated that up to half of global methane (CH4) emissions are derived from microbial processes in aquatic ecosystems. However, it is not fully understood which factors explain the spatial and temporal variability of these emissions. For example, light has previously been shown to bo...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-07-01
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| Series: | Environmental DNA |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/edn3.441 |
| _version_ | 1797770537439592448 |
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| author | Elias Broman Rinti Barua Daniel Donald Florian Roth Christoph Humborg Alf Norkko Tom Jilbert Stefano Bonaglia Francisco J. A. Nascimento |
| author_facet | Elias Broman Rinti Barua Daniel Donald Florian Roth Christoph Humborg Alf Norkko Tom Jilbert Stefano Bonaglia Francisco J. A. Nascimento |
| author_sort | Elias Broman |
| collection | DOAJ |
| description | Abstract It is estimated that up to half of global methane (CH4) emissions are derived from microbial processes in aquatic ecosystems. However, it is not fully understood which factors explain the spatial and temporal variability of these emissions. For example, light has previously been shown to both inhibit and stimulate aerobic methane‐oxidizing bacteria (i.e., methanotrophs) in the water column. These contrasting results indicate that the mechanisms that light has on CH4 oxidation are not yet clearly known, even less so for benthic aerobic methanotrophs. Here, we tested whether light reaching the seafloor can inhibit methanotrophic activity on the sediment surface. We sampled and distributed over 40 intact sediment cores from two coastal sites (illuminated 10 m, and a dark site at 33 m water depth) into 0, 50, and 100 PAR light treatments. After 10 days, we found no difference between treatments for each site in pore‐water CH4 concentrations, relative abundance of aerobic methanotrophs, or the number of RNA transcripts related to methane oxidation. Our results suggest that light attenuation in coastal waters does not significantly affect aerobic methanotrophs in coastal sediments. |
| first_indexed | 2024-03-12T21:24:38Z |
| format | Article |
| id | doaj.art-61c471c9e4584ec4b2feb6df4611329a |
| institution | Directory Open Access Journal |
| issn | 2637-4943 |
| language | English |
| last_indexed | 2024-03-12T21:24:38Z |
| publishDate | 2023-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Environmental DNA |
| spelling | doaj.art-61c471c9e4584ec4b2feb6df4611329a2023-07-28T10:02:53ZengWileyEnvironmental DNA2637-49432023-07-015476678110.1002/edn3.441No evidence of light inhibition on aerobic methanotrophs in coastal sediments using eDNA and eRNAElias Broman0Rinti Barua1Daniel Donald2Florian Roth3Christoph Humborg4Alf Norkko5Tom Jilbert6Stefano Bonaglia7Francisco J. A. Nascimento8Department of Ecology, Environment and Plant Sciences Stockholm University Stockholm SwedenDepartment of Ecology, Environment and Plant Sciences Stockholm University Stockholm SwedenTvärminne Zoological Station, Faculty of Biological of Environmental Sciences University of Helsinki Helsinki FinlandBaltic Sea Centre Stockholm University Stockholm SwedenBaltic Sea Centre Stockholm University Stockholm SwedenBaltic Sea Centre Stockholm University Stockholm SwedenTvärminne Zoological Station, Faculty of Biological of Environmental Sciences University of Helsinki Helsinki FinlandDepartment of Marine Sciences University of Gothenburg Gothenburg SwedenDepartment of Ecology, Environment and Plant Sciences Stockholm University Stockholm SwedenAbstract It is estimated that up to half of global methane (CH4) emissions are derived from microbial processes in aquatic ecosystems. However, it is not fully understood which factors explain the spatial and temporal variability of these emissions. For example, light has previously been shown to both inhibit and stimulate aerobic methane‐oxidizing bacteria (i.e., methanotrophs) in the water column. These contrasting results indicate that the mechanisms that light has on CH4 oxidation are not yet clearly known, even less so for benthic aerobic methanotrophs. Here, we tested whether light reaching the seafloor can inhibit methanotrophic activity on the sediment surface. We sampled and distributed over 40 intact sediment cores from two coastal sites (illuminated 10 m, and a dark site at 33 m water depth) into 0, 50, and 100 PAR light treatments. After 10 days, we found no difference between treatments for each site in pore‐water CH4 concentrations, relative abundance of aerobic methanotrophs, or the number of RNA transcripts related to methane oxidation. Our results suggest that light attenuation in coastal waters does not significantly affect aerobic methanotrophs in coastal sediments.https://doi.org/10.1002/edn3.441benthicDNAmethanePARpmoARNA |
| spellingShingle | Elias Broman Rinti Barua Daniel Donald Florian Roth Christoph Humborg Alf Norkko Tom Jilbert Stefano Bonaglia Francisco J. A. Nascimento No evidence of light inhibition on aerobic methanotrophs in coastal sediments using eDNA and eRNA Environmental DNA benthic DNA methane PAR pmoA RNA |
| title | No evidence of light inhibition on aerobic methanotrophs in coastal sediments using eDNA and eRNA |
| title_full | No evidence of light inhibition on aerobic methanotrophs in coastal sediments using eDNA and eRNA |
| title_fullStr | No evidence of light inhibition on aerobic methanotrophs in coastal sediments using eDNA and eRNA |
| title_full_unstemmed | No evidence of light inhibition on aerobic methanotrophs in coastal sediments using eDNA and eRNA |
| title_short | No evidence of light inhibition on aerobic methanotrophs in coastal sediments using eDNA and eRNA |
| title_sort | no evidence of light inhibition on aerobic methanotrophs in coastal sediments using edna and erna |
| topic | benthic DNA methane PAR pmoA RNA |
| url | https://doi.org/10.1002/edn3.441 |
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