A physiological study of integument secretions in the marine polychaete <i>Eulalia viridis</i> and their potential biotechnological value.
There is a growing interest in marine bioproducts, especially in those with life improving potential to humans. Marine invertebrates, in particular, possess particular interest due to their high diversity, abundance and ecological importance. However, the physiology of such a wide and diverse group...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2014-06-01
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| Series: | Frontiers in Marine Science |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/conf.fmars.2014.02.00040/full |
| _version_ | 1819144005504466944 |
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| author | Ana Patrícia Rodrigo António Pedro Alves de Matos Francisco Carrapiço |
| author_facet | Ana Patrícia Rodrigo António Pedro Alves de Matos Francisco Carrapiço |
| author_sort | Ana Patrícia Rodrigo |
| collection | DOAJ |
| description | There is a growing interest in marine bioproducts, especially in those with life improving potential to humans. Marine invertebrates, in particular, possess particular interest due to their high diversity, abundance and ecological importance. However, the physiology of such a wide and diverse group of organisms is far from understood. Marine annelids, in particular (Class Polychaeta), are of especial interest, in large part owing to their ecological representativity and easy handling. Still only few studies have been focusing on physiological traits of the polychaeta that may relate to the production of bioproducts of potential biotechnological applications. The secretion of substances by polychaetes is acknowledged to relate primarily with locomotion and feeding, but also thought to have an important role in egg protection, prevention against infection and even as defence mechanisms against possible predators. As such, the present work aims at identifying, isolating and analysing the nature of integumentary secretions of <i>E. viridis</i>, a common intertidal worm of rocky shores. Histological analyses revealed a more complex microanatomy of the skin than it could be anticipated. Several cell types were identified in the integument, each bearing different functions such as structural support, pigmentation and sensorial, to which is added secretion as one of the most important roles. Due to the great complexity of the integument, the identification of the chemical nature of all types of secretions is particularly challenging. However, more detailed histochemical analyses and electron microscopy enabled the identification of different secretory cells and their chemical nature and role. The analyses was complemented by protein isolation from mucous and homogenates trough SDS-PAGE. Extracts revealed, for instance, that peptidic substances produced by the skin are potentially biocidal, as assessed through the Microtox Assay. Overall, the results enhance the importance of studies with marine invertebrates, especially polychaetes, for the isolation of natural biocides, antioxidants and other substances of high biotechnological value. However, further research is still needed to fully isolate and identify such substances and the physiological processes underlying their production in marine invertebrates in general. |
| first_indexed | 2024-12-22T12:35:15Z |
| format | Article |
| id | doaj.art-61c4752ac9af453bb18efb5c37840d84 |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-12-22T12:35:15Z |
| publishDate | 2014-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-61c4752ac9af453bb18efb5c37840d842022-12-21T18:25:36ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452014-06-01110.3389/conf.fmars.2014.02.00040101906A physiological study of integument secretions in the marine polychaete <i>Eulalia viridis</i> and their potential biotechnological value.Ana Patrícia Rodrigo0António Pedro Alves de Matos1Francisco Carrapiço2IMAR – Instituto do MarCESAM-Centro de Estudos do Ambiente e do MarCentro de Biologia AmbientalThere is a growing interest in marine bioproducts, especially in those with life improving potential to humans. Marine invertebrates, in particular, possess particular interest due to their high diversity, abundance and ecological importance. However, the physiology of such a wide and diverse group of organisms is far from understood. Marine annelids, in particular (Class Polychaeta), are of especial interest, in large part owing to their ecological representativity and easy handling. Still only few studies have been focusing on physiological traits of the polychaeta that may relate to the production of bioproducts of potential biotechnological applications. The secretion of substances by polychaetes is acknowledged to relate primarily with locomotion and feeding, but also thought to have an important role in egg protection, prevention against infection and even as defence mechanisms against possible predators. As such, the present work aims at identifying, isolating and analysing the nature of integumentary secretions of <i>E. viridis</i>, a common intertidal worm of rocky shores. Histological analyses revealed a more complex microanatomy of the skin than it could be anticipated. Several cell types were identified in the integument, each bearing different functions such as structural support, pigmentation and sensorial, to which is added secretion as one of the most important roles. Due to the great complexity of the integument, the identification of the chemical nature of all types of secretions is particularly challenging. However, more detailed histochemical analyses and electron microscopy enabled the identification of different secretory cells and their chemical nature and role. The analyses was complemented by protein isolation from mucous and homogenates trough SDS-PAGE. Extracts revealed, for instance, that peptidic substances produced by the skin are potentially biocidal, as assessed through the Microtox Assay. Overall, the results enhance the importance of studies with marine invertebrates, especially polychaetes, for the isolation of natural biocides, antioxidants and other substances of high biotechnological value. However, further research is still needed to fully isolate and identify such substances and the physiological processes underlying their production in marine invertebrates in general.http://journal.frontiersin.org/Journal/10.3389/conf.fmars.2014.02.00040/fullAnnelidaGoblet Cellsmarine biotechnologyNatural ProductsBiocidals |
| spellingShingle | Ana Patrícia Rodrigo António Pedro Alves de Matos Francisco Carrapiço A physiological study of integument secretions in the marine polychaete <i>Eulalia viridis</i> and their potential biotechnological value. Frontiers in Marine Science Annelida Goblet Cells marine biotechnology Natural Products Biocidals |
| title | A physiological study of integument secretions in the marine polychaete <i>Eulalia viridis</i> and their potential biotechnological value. |
| title_full | A physiological study of integument secretions in the marine polychaete <i>Eulalia viridis</i> and their potential biotechnological value. |
| title_fullStr | A physiological study of integument secretions in the marine polychaete <i>Eulalia viridis</i> and their potential biotechnological value. |
| title_full_unstemmed | A physiological study of integument secretions in the marine polychaete <i>Eulalia viridis</i> and their potential biotechnological value. |
| title_short | A physiological study of integument secretions in the marine polychaete <i>Eulalia viridis</i> and their potential biotechnological value. |
| title_sort | physiological study of integument secretions in the marine polychaete i eulalia viridis i and their potential biotechnological value |
| topic | Annelida Goblet Cells marine biotechnology Natural Products Biocidals |
| url | http://journal.frontiersin.org/Journal/10.3389/conf.fmars.2014.02.00040/full |
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