Identification and Expression Analysis of Chloroplast p-psbB Gene Differentially Expressed in Wild Ginseng
Panax ginseng is a well-known herbal medicine in traditional Asian medicine. Although wild ginseng is widely accepted to be more active than cultivated ginseng in chemoprevention,little has actually been reported on the difference between wild ginseng and cultivated ginseng. Using suppressive subtra...
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| Format: | Article |
| Language: | English |
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Korean Pharmacopuncture Institute
2012-03-01
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| Series: | Journal of Pharmacopuncture |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.3831/KPI.2012.15.1.018 |
| _version_ | 1811266355080462336 |
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| author | Doo-Young Kim Ki-Rok Kwon Won-Mo Kang Eun-Yi Jeon Jun-Hyeog Jang |
| author_facet | Doo-Young Kim Ki-Rok Kwon Won-Mo Kang Eun-Yi Jeon Jun-Hyeog Jang |
| author_sort | Doo-Young Kim |
| collection | DOAJ |
| description | Panax ginseng is a well-known herbal medicine in traditional Asian medicine. Although wild ginseng is widely accepted to be more active than cultivated ginseng in chemoprevention,little has actually been reported on the difference between wild ginseng and cultivated ginseng. Using suppressive subtraction hybridization, we cloned the p-psbB gene as a candidate target gene for a wild ginseng-specific gene. Here, we report that one of the clones isolated in this screen was the chloroplast p-psbB gene, a chlorophyll a-binding inner antenna protein in the photosystem II complex, located in the lipid matrix of the thylakoid membrane. Real-time results showed that the expression of the p-psbB gene was significantly up-regulated in wild ginseng as compared to cultivated ginseng. Thus, the p-psbB gene may be one of the important markers of wild ginseng. |
| first_indexed | 2024-04-12T20:40:28Z |
| format | Article |
| id | doaj.art-61c5a349cbde4797b922a813222ac10b |
| institution | Directory Open Access Journal |
| issn | 2093-6966 2234-6856 |
| language | English |
| last_indexed | 2024-04-12T20:40:28Z |
| publishDate | 2012-03-01 |
| publisher | Korean Pharmacopuncture Institute |
| record_format | Article |
| series | Journal of Pharmacopuncture |
| spelling | doaj.art-61c5a349cbde4797b922a813222ac10b2022-12-22T03:17:25ZengKorean Pharmacopuncture InstituteJournal of Pharmacopuncture2093-69662234-68562012-03-01151182210.3831/KPI.2012.15.1.018Identification and Expression Analysis of Chloroplast p-psbB Gene Differentially Expressed in Wild GinsengDoo-Young Kim0Ki-Rok Kwon1Won-Mo Kang2Eun-Yi Jeon3Jun-Hyeog Jang 4Graduate School of Oriental Medicine, Sangji University, Wonju, KoreaResearch Center of the Korean Pharmacopuncture Institute, Seoul, KoreaDepartment of Biochemistry, Graduate School of Medicine, Inha University, Incheon, Korea Department of Biochemistry, Graduate School of Medicine, Inha University, Incheon, Korea Department of Biochemistry, Graduate School of Medicine, Inha University, Incheon, Korea Panax ginseng is a well-known herbal medicine in traditional Asian medicine. Although wild ginseng is widely accepted to be more active than cultivated ginseng in chemoprevention,little has actually been reported on the difference between wild ginseng and cultivated ginseng. Using suppressive subtraction hybridization, we cloned the p-psbB gene as a candidate target gene for a wild ginseng-specific gene. Here, we report that one of the clones isolated in this screen was the chloroplast p-psbB gene, a chlorophyll a-binding inner antenna protein in the photosystem II complex, located in the lipid matrix of the thylakoid membrane. Real-time results showed that the expression of the p-psbB gene was significantly up-regulated in wild ginseng as compared to cultivated ginseng. Thus, the p-psbB gene may be one of the important markers of wild ginseng.http://dx.doi.org/10.3831/KPI.2012.15.1.018Cultivated ginsengPCRp-psbB genesuppressive subtraction hybridization (SSH)wild ginseng |
| spellingShingle | Doo-Young Kim Ki-Rok Kwon Won-Mo Kang Eun-Yi Jeon Jun-Hyeog Jang Identification and Expression Analysis of Chloroplast p-psbB Gene Differentially Expressed in Wild Ginseng Journal of Pharmacopuncture Cultivated ginseng PCR p-psbB gene suppressive subtraction hybridization (SSH) wild ginseng |
| title | Identification and Expression Analysis of Chloroplast p-psbB Gene Differentially Expressed in Wild Ginseng |
| title_full | Identification and Expression Analysis of Chloroplast p-psbB Gene Differentially Expressed in Wild Ginseng |
| title_fullStr | Identification and Expression Analysis of Chloroplast p-psbB Gene Differentially Expressed in Wild Ginseng |
| title_full_unstemmed | Identification and Expression Analysis of Chloroplast p-psbB Gene Differentially Expressed in Wild Ginseng |
| title_short | Identification and Expression Analysis of Chloroplast p-psbB Gene Differentially Expressed in Wild Ginseng |
| title_sort | identification and expression analysis of chloroplast p psbb gene differentially expressed in wild ginseng |
| topic | Cultivated ginseng PCR p-psbB gene suppressive subtraction hybridization (SSH) wild ginseng |
| url | http://dx.doi.org/10.3831/KPI.2012.15.1.018 |
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