Toxic Effects of Methotrexate on Rat Kidney Recovered by Crocin as a Consequence of Antioxidant Activity and Lipid Peroxidation Prevention
Background: The application of methotrexate (MTX) as a chemotherapy agent and immune system suppressant has various side effects. Crocin, a xanthine derivative plant, has many therapeutic benefits. This study was planned to assess the effect of crocin on renal toxicity of MTX in a rat model. Methods...
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| Format: | Article |
| Language: | English |
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Pasteur Institute of Iran
2020-01-01
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| Series: | Iranian Biomedical Journal |
| Subjects: | |
| Online Access: | http://ibj.pasteur.ac.ir/article-1-2868-en.html |
| _version_ | 1818264304537829376 |
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| author | Cyrus Jalili Ali Ghanbari Shiva Roshankhah Mohammad Reza Salahshoor |
| author_facet | Cyrus Jalili Ali Ghanbari Shiva Roshankhah Mohammad Reza Salahshoor |
| author_sort | Cyrus Jalili |
| collection | DOAJ |
| description | Background: The application of methotrexate (MTX) as a chemotherapy agent and immune system suppressant has various side effects. Crocin, a xanthine derivative plant, has many therapeutic benefits. This study was planned to assess the effect of crocin on renal toxicity of MTX in a rat model. Methods: Forty eight rats were divided randomly into eight groups (n = 6), which received normal saline, MTX, crocin, and MTX + crocin for 28 days intraperitoneally. The levels of oxidative stress in kidney and blood serum were measured, and the kidney was analyzed histologically. Results: MTX caused an enhancement in the levels of thiobarbituric acid reactive substances and biochemical marker (creatinine and BUN). Besides, a significant decrease was observed in tissue parameters and antioxidant capacity compared to the normal control group (p < 0.001). The crocin and crocin + MTX decreased the biochemical markers, the levels of thiobarbituric acid reactive species, and tissue parameters considerably at entire dose (12.5, 25, and 50 mg/kg) and enhanced the antioxidant capacity levels compared to the MTX group (p < 0.001). Conclusion: Administration of crocin improves the damage caused by MTX in rats. The crocin by the establishment of balance in the levels of antioxidant prevents the damage to the renal cell membrane, and subsequently the renal damage repairs. |
| first_indexed | 2024-12-12T19:32:47Z |
| format | Article |
| id | doaj.art-61c8b757e616475bbe46b0bec70ab3d2 |
| institution | Directory Open Access Journal |
| issn | 1028-852X 2008-823X |
| language | English |
| last_indexed | 2024-12-12T19:32:47Z |
| publishDate | 2020-01-01 |
| publisher | Pasteur Institute of Iran |
| record_format | Article |
| series | Iranian Biomedical Journal |
| spelling | doaj.art-61c8b757e616475bbe46b0bec70ab3d22022-12-22T00:14:23ZengPasteur Institute of IranIranian Biomedical Journal1028-852X2008-823X2020-01-012413946Toxic Effects of Methotrexate on Rat Kidney Recovered by Crocin as a Consequence of Antioxidant Activity and Lipid Peroxidation PreventionCyrus Jalili0Ali Ghanbari1Shiva Roshankhah2Mohammad Reza Salahshoor3 Medical Biology Research Center, Department of Anatomical Sciences, Kermanshah University of Medical Sciences, Daneshgah Ave., Taghbostan, Kermanshah, Iran Department of Anatomical Sciences, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran Department of Anatomical Sciences, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran Department of Anatomical Sciences, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran Background: The application of methotrexate (MTX) as a chemotherapy agent and immune system suppressant has various side effects. Crocin, a xanthine derivative plant, has many therapeutic benefits. This study was planned to assess the effect of crocin on renal toxicity of MTX in a rat model. Methods: Forty eight rats were divided randomly into eight groups (n = 6), which received normal saline, MTX, crocin, and MTX + crocin for 28 days intraperitoneally. The levels of oxidative stress in kidney and blood serum were measured, and the kidney was analyzed histologically. Results: MTX caused an enhancement in the levels of thiobarbituric acid reactive substances and biochemical marker (creatinine and BUN). Besides, a significant decrease was observed in tissue parameters and antioxidant capacity compared to the normal control group (p < 0.001). The crocin and crocin + MTX decreased the biochemical markers, the levels of thiobarbituric acid reactive species, and tissue parameters considerably at entire dose (12.5, 25, and 50 mg/kg) and enhanced the antioxidant capacity levels compared to the MTX group (p < 0.001). Conclusion: Administration of crocin improves the damage caused by MTX in rats. The crocin by the establishment of balance in the levels of antioxidant prevents the damage to the renal cell membrane, and subsequently the renal damage repairs.http://ibj.pasteur.ac.ir/article-1-2868-en.htmlantioxidantscrocinkidneymethotrexate |
| spellingShingle | Cyrus Jalili Ali Ghanbari Shiva Roshankhah Mohammad Reza Salahshoor Toxic Effects of Methotrexate on Rat Kidney Recovered by Crocin as a Consequence of Antioxidant Activity and Lipid Peroxidation Prevention Iranian Biomedical Journal antioxidants crocin kidney methotrexate |
| title | Toxic Effects of Methotrexate on Rat Kidney Recovered by Crocin as a Consequence of Antioxidant Activity and Lipid Peroxidation Prevention |
| title_full | Toxic Effects of Methotrexate on Rat Kidney Recovered by Crocin as a Consequence of Antioxidant Activity and Lipid Peroxidation Prevention |
| title_fullStr | Toxic Effects of Methotrexate on Rat Kidney Recovered by Crocin as a Consequence of Antioxidant Activity and Lipid Peroxidation Prevention |
| title_full_unstemmed | Toxic Effects of Methotrexate on Rat Kidney Recovered by Crocin as a Consequence of Antioxidant Activity and Lipid Peroxidation Prevention |
| title_short | Toxic Effects of Methotrexate on Rat Kidney Recovered by Crocin as a Consequence of Antioxidant Activity and Lipid Peroxidation Prevention |
| title_sort | toxic effects of methotrexate on rat kidney recovered by crocin as a consequence of antioxidant activity and lipid peroxidation prevention |
| topic | antioxidants crocin kidney methotrexate |
| url | http://ibj.pasteur.ac.ir/article-1-2868-en.html |
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