Identifications of QTLs and Candidate Genes Associated with <i>Pseudomonas syringae</i> Responses in Cultivated Soybean (<i>Glycine max</i>) and Wild Soybean (<i>Glycine soja</i>)
Soybeans (<i>Glycine max</i>) are a key food crop, serving as a valuable source of both oil and plant-derived protein. <i>Pseudomonas syringae pv. glycinea</i> (<i>Psg</i>) is among the most aggressive and prevalent pathogens affecting soybean production, causing...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-02-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/5/4618 |
| Summary: | Soybeans (<i>Glycine max</i>) are a key food crop, serving as a valuable source of both oil and plant-derived protein. <i>Pseudomonas syringae pv. glycinea</i> (<i>Psg</i>) is among the most aggressive and prevalent pathogens affecting soybean production, causing a form of bacterial spot disease that impacts soybean leaves and thereby reduces crop yields. In this study, 310 natural soybean varieties were screened for <i>Psg</i> resistance and susceptibility. The identified susceptible and resistant varieties were then used for linkage mapping, BSA-seq, and whole genome sequencing (WGS) analyses aimed at identifying key QTLs associated with <i>Psg</i> responses. Candidate <i>Psg</i>-related genes were further confirmed through WGS and qPCR analyses. Candidate gene haplotype analyses were used to explore the associations between haplotypes and soybean <i>Psg</i> resistance. In addition, landrace and wild soybean plants were found to exhibit a higher degree of <i>Psg</i> resistance as compared to cultivated soybean varieties. In total, 10 QTLs were identified using chromosome segment substitution lines derived from Suinong14 (cultivated soybean) and ZYD00006 (wild soybean). <i>Glyma.10g230200</i> was found to be induced in response to <i>Psg</i>, with the <i>Glyma.10g230200</i> haplotype corresponding to soybean disease resistance. The QTLs identified herein can be leveraged to guide the marker-assisted breeding of soybean cultivars that exhibit partial resistance to <i>Psg</i>. Moreover, further functional and molecular studies of <i>Glyma.10g230200</i> have the potential to offer insight into the mechanistic basis for soybean <i>Psg</i> resistance. |
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| ISSN: | 1661-6596 1422-0067 |