Enhanced Glutathione Metabolism Is Correlated with Sulfur-Induced Resistance in Tobacco mosaic virus–Infected Genetically Susceptible Nicotiana tabacum Plants
Sulfur-induced resistance, also known as sulfur-enhanced defense (SIR/SED) was investigated in Nicotiana tabacum cv. Samsun nn during compatible interaction with Tobacco mosaic virus (TMV) in correlation with glutathione metabolism. To evaluate the influence of sulfur nutritional status on virus inf...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2010-11-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-05-10-0117 |
| _version_ | 1811291331632300032 |
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| author | Kerstin Höller Lóránt Király András Künstler Maria Müller Gábor Gullner Maria Fattinger Bernd Zechmann |
| author_facet | Kerstin Höller Lóránt Király András Künstler Maria Müller Gábor Gullner Maria Fattinger Bernd Zechmann |
| author_sort | Kerstin Höller |
| collection | DOAJ |
| description | Sulfur-induced resistance, also known as sulfur-enhanced defense (SIR/SED) was investigated in Nicotiana tabacum cv. Samsun nn during compatible interaction with Tobacco mosaic virus (TMV) in correlation with glutathione metabolism. To evaluate the influence of sulfur nutritional status on virus infection, tobacco plants were treated with nutrient solutions containing either sufficient sulfate (+S) or no sulfate (−S). Sufficient sulfate supply resulted in a suppressed and delayed symptom development and diminished virus accumulation over a period of 14 days after inoculation as compared with −S conditions. Expression of the defense marker gene PR-1a was markedly upregulated in sulfate-treated plants during the first day after TMV inoculation. The occurrence of SIR/SED correlated with a higher level of activity of sulfate assimilation, cysteine, and glutathione metabolism in plants treated with sulfate. Additionally, two key genes involved in cysteine and glutathione biosynthesis (encoding adenosine 5′-phosphosulfate reductase and γ-glutamylcysteine synthetase, respectively) were upregulated within the first day after TMV inoculation under +S conditions. Sulfate withdrawal from the soil was accelerated at the beginning of the infection, whereas it declined in the long term, leading to an accumulation of sulfur in the soil of plants grown with sulfate. This observation could be correlated with a decrease in sulfur contents in TMV-infected leaves in the long term. In summary, this is the first study that demonstrates a link between the activation of cysteine and glutathione metabolism and the induction of SIR/SED during a compatible plant-virus interaction in tobacco plants, indicating a general mechanism behind SIR/SED. |
| first_indexed | 2024-04-13T04:27:37Z |
| format | Article |
| id | doaj.art-61cae67ad3994c09af9478909ae432c2 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T04:27:37Z |
| publishDate | 2010-11-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-61cae67ad3994c09af9478909ae432c22022-12-22T03:02:27ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062010-11-0123111448145910.1094/MPMI-05-10-0117Enhanced Glutathione Metabolism Is Correlated with Sulfur-Induced Resistance in Tobacco mosaic virus–Infected Genetically Susceptible Nicotiana tabacum PlantsKerstin HöllerLóránt KirályAndrás KünstlerMaria MüllerGábor GullnerMaria FattingerBernd ZechmannSulfur-induced resistance, also known as sulfur-enhanced defense (SIR/SED) was investigated in Nicotiana tabacum cv. Samsun nn during compatible interaction with Tobacco mosaic virus (TMV) in correlation with glutathione metabolism. To evaluate the influence of sulfur nutritional status on virus infection, tobacco plants were treated with nutrient solutions containing either sufficient sulfate (+S) or no sulfate (−S). Sufficient sulfate supply resulted in a suppressed and delayed symptom development and diminished virus accumulation over a period of 14 days after inoculation as compared with −S conditions. Expression of the defense marker gene PR-1a was markedly upregulated in sulfate-treated plants during the first day after TMV inoculation. The occurrence of SIR/SED correlated with a higher level of activity of sulfate assimilation, cysteine, and glutathione metabolism in plants treated with sulfate. Additionally, two key genes involved in cysteine and glutathione biosynthesis (encoding adenosine 5′-phosphosulfate reductase and γ-glutamylcysteine synthetase, respectively) were upregulated within the first day after TMV inoculation under +S conditions. Sulfate withdrawal from the soil was accelerated at the beginning of the infection, whereas it declined in the long term, leading to an accumulation of sulfur in the soil of plants grown with sulfate. This observation could be correlated with a decrease in sulfur contents in TMV-infected leaves in the long term. In summary, this is the first study that demonstrates a link between the activation of cysteine and glutathione metabolism and the induction of SIR/SED during a compatible plant-virus interaction in tobacco plants, indicating a general mechanism behind SIR/SED.https://apsjournals.apsnet.org/doi/10.1094/MPMI-05-10-0117 |
| spellingShingle | Kerstin Höller Lóránt Király András Künstler Maria Müller Gábor Gullner Maria Fattinger Bernd Zechmann Enhanced Glutathione Metabolism Is Correlated with Sulfur-Induced Resistance in Tobacco mosaic virus–Infected Genetically Susceptible Nicotiana tabacum Plants Molecular Plant-Microbe Interactions |
| title | Enhanced Glutathione Metabolism Is Correlated with Sulfur-Induced Resistance in Tobacco mosaic virus–Infected Genetically Susceptible Nicotiana tabacum Plants |
| title_full | Enhanced Glutathione Metabolism Is Correlated with Sulfur-Induced Resistance in Tobacco mosaic virus–Infected Genetically Susceptible Nicotiana tabacum Plants |
| title_fullStr | Enhanced Glutathione Metabolism Is Correlated with Sulfur-Induced Resistance in Tobacco mosaic virus–Infected Genetically Susceptible Nicotiana tabacum Plants |
| title_full_unstemmed | Enhanced Glutathione Metabolism Is Correlated with Sulfur-Induced Resistance in Tobacco mosaic virus–Infected Genetically Susceptible Nicotiana tabacum Plants |
| title_short | Enhanced Glutathione Metabolism Is Correlated with Sulfur-Induced Resistance in Tobacco mosaic virus–Infected Genetically Susceptible Nicotiana tabacum Plants |
| title_sort | enhanced glutathione metabolism is correlated with sulfur induced resistance in tobacco mosaic virus infected genetically susceptible nicotiana tabacum plants |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-05-10-0117 |
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