The effect of silver nanoparticles on apoptosis and dark neuron production in rat hippocampus
Objective(s):Silver nanoparticles (Ag-NPs) are used widely in bedding, water purification, tooth paste and toys. These nanoparticles can enter into the body and move into the hippocampus. The aim of this study was to investigate the neurotoxicity of silver nanoparticles in the adult rat hippocampus....
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2015-07-01
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| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | http://ijbms.mums.ac.ir/pdf_4644_fa468ad8a423d7958d11092dd2b0877d.html |
| _version_ | 1818380470901014528 |
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| author | Farzaneh Bagheri-abassi Hassan Alavi Abbas Mohammadipour Fatemeh Motejaded Alireza Ebrahimzadeh-bideskan |
| author_facet | Farzaneh Bagheri-abassi Hassan Alavi Abbas Mohammadipour Fatemeh Motejaded Alireza Ebrahimzadeh-bideskan |
| author_sort | Farzaneh Bagheri-abassi |
| collection | DOAJ |
| description | Objective(s):Silver nanoparticles (Ag-NPs) are used widely in bedding, water purification, tooth paste and toys. These nanoparticles can enter into the body and move into the hippocampus. The aim of this study was to investigate the neurotoxicity of silver nanoparticles in the adult rat hippocampus. Materials and Methods:12 male Wistar rats were randomly divided into two experimental and control groups (6 rats in each group). Animals in the experimental group received Ag-NPs (30 mg/kg) orally (gavage) for 28 consecutive days. Control group in the same period was treated with distilled water via gavage. At the end of experiment, animals were deeply anesthetized, sacrificed, and their brains were collected from each group. Finally the brain sections were stained using toluidine blue and TUNEL. Then to compare the groups, dark neurons (DNs) and apoptotic neurons were counted by morphometric method. Results: Results showed that the numbers of DNs and apoptotic cells in the CA1, CA2, CA3, and dentate gyrus (DG) of hippocampus significantly increased in the Ag-NPs group in comparison to the control group (P |
| first_indexed | 2024-12-14T02:19:12Z |
| format | Article |
| id | doaj.art-61ccf23f55914c42aa3e27909a337ecc |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-14T02:19:12Z |
| publishDate | 2015-07-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-61ccf23f55914c42aa3e27909a337ecc2022-12-21T23:20:33ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742015-07-011876446484644The effect of silver nanoparticles on apoptosis and dark neuron production in rat hippocampusFarzaneh Bagheri-abassi0Hassan Alavi1Abbas Mohammadipour2Fatemeh Motejaded3Alireza Ebrahimzadeh-bideskan4Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranElectronic Microscope Center, Buali Institute, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranObjective(s):Silver nanoparticles (Ag-NPs) are used widely in bedding, water purification, tooth paste and toys. These nanoparticles can enter into the body and move into the hippocampus. The aim of this study was to investigate the neurotoxicity of silver nanoparticles in the adult rat hippocampus. Materials and Methods:12 male Wistar rats were randomly divided into two experimental and control groups (6 rats in each group). Animals in the experimental group received Ag-NPs (30 mg/kg) orally (gavage) for 28 consecutive days. Control group in the same period was treated with distilled water via gavage. At the end of experiment, animals were deeply anesthetized, sacrificed, and their brains were collected from each group. Finally the brain sections were stained using toluidine blue and TUNEL. Then to compare the groups, dark neurons (DNs) and apoptotic neurons were counted by morphometric method. Results: Results showed that the numbers of DNs and apoptotic cells in the CA1, CA2, CA3, and dentate gyrus (DG) of hippocampus significantly increased in the Ag-NPs group in comparison to the control group (Phttp://ijbms.mums.ac.ir/pdf_4644_fa468ad8a423d7958d11092dd2b0877d.htmlApoptosisDark neuronHippocampusNeurotoxicitySilver nanoparticles |
| spellingShingle | Farzaneh Bagheri-abassi Hassan Alavi Abbas Mohammadipour Fatemeh Motejaded Alireza Ebrahimzadeh-bideskan The effect of silver nanoparticles on apoptosis and dark neuron production in rat hippocampus Iranian Journal of Basic Medical Sciences Apoptosis Dark neuron Hippocampus Neurotoxicity Silver nanoparticles |
| title | The effect of silver nanoparticles on apoptosis and dark neuron production in rat hippocampus |
| title_full | The effect of silver nanoparticles on apoptosis and dark neuron production in rat hippocampus |
| title_fullStr | The effect of silver nanoparticles on apoptosis and dark neuron production in rat hippocampus |
| title_full_unstemmed | The effect of silver nanoparticles on apoptosis and dark neuron production in rat hippocampus |
| title_short | The effect of silver nanoparticles on apoptosis and dark neuron production in rat hippocampus |
| title_sort | effect of silver nanoparticles on apoptosis and dark neuron production in rat hippocampus |
| topic | Apoptosis Dark neuron Hippocampus Neurotoxicity Silver nanoparticles |
| url | http://ijbms.mums.ac.ir/pdf_4644_fa468ad8a423d7958d11092dd2b0877d.html |
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