Unraveling the celiac disease-related immunogenic complexes in a set of wheat and tritordeum genotypes: implications for low-gluten precision breeding in cereal crops
The development of low-gluten immunogenic cereal varieties is a suitable way to fight the increment of pathologies associated with the consumption of cereals. Although RNAi and CRISPR/Cas technologies were effective in providing low-gluten wheat, the regulatory framework, particularly in the Europea...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-05-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1171882/full |
| _version_ | 1797829273206128640 |
|---|---|
| author | Miriam Marín-Sanz Francisco Barro Susana Sánchez-León |
| author_facet | Miriam Marín-Sanz Francisco Barro Susana Sánchez-León |
| author_sort | Miriam Marín-Sanz |
| collection | DOAJ |
| description | The development of low-gluten immunogenic cereal varieties is a suitable way to fight the increment of pathologies associated with the consumption of cereals. Although RNAi and CRISPR/Cas technologies were effective in providing low-gluten wheat, the regulatory framework, particularly in the European Union, is an obstacle to the short- or medium-term implementation of such lines. In the present work, we carried out a high throughput amplicon sequencing of two highly immunogenic complexes of wheat gliadins in a set of bread and durum wheat, and tritordeum genotypes. Bread wheat genotypes harboring the 1BL/1RS translocation were included in the analysis and their amplicons successfully identified. The number of CD epitopes and their abundances were determined in the alpha- and gamma-gliadin amplicons, including 40k-γ-secalin ones. Bread wheat genotypes not containing the 1BL/1RS translocation showed a higher average number of both alpha- and gamma-gliadin epitopes than those containing such translocation. Interestingly, alpha-gliadin amplicons not containing CD epitopes accounted for the highest abundance (around 53%), and the alpha- and gamma-gliadin amplicons with the highest number of epitopes were present in the D-subgenome. The durum wheat and tritordeum genotypes showed the lowest number of alpha- and gamma-gliadin CD epitopes. Our results allow progress in unraveling the immunogenic complexes of alpha- and gamma-gliadins and can contribute to the development of low-immunogenic varieties within precision breeding programs, by crossing or by CRISPR/Cas gene editing. |
| first_indexed | 2024-04-09T13:17:52Z |
| format | Article |
| id | doaj.art-61cdb60b338c4072a7fb0aa19e2dc4b2 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-09T13:17:52Z |
| publishDate | 2023-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-61cdb60b338c4072a7fb0aa19e2dc4b22023-05-11T12:19:21ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-05-011410.3389/fpls.2023.11718821171882Unraveling the celiac disease-related immunogenic complexes in a set of wheat and tritordeum genotypes: implications for low-gluten precision breeding in cereal cropsMiriam Marín-SanzFrancisco BarroSusana Sánchez-LeónThe development of low-gluten immunogenic cereal varieties is a suitable way to fight the increment of pathologies associated with the consumption of cereals. Although RNAi and CRISPR/Cas technologies were effective in providing low-gluten wheat, the regulatory framework, particularly in the European Union, is an obstacle to the short- or medium-term implementation of such lines. In the present work, we carried out a high throughput amplicon sequencing of two highly immunogenic complexes of wheat gliadins in a set of bread and durum wheat, and tritordeum genotypes. Bread wheat genotypes harboring the 1BL/1RS translocation were included in the analysis and their amplicons successfully identified. The number of CD epitopes and their abundances were determined in the alpha- and gamma-gliadin amplicons, including 40k-γ-secalin ones. Bread wheat genotypes not containing the 1BL/1RS translocation showed a higher average number of both alpha- and gamma-gliadin epitopes than those containing such translocation. Interestingly, alpha-gliadin amplicons not containing CD epitopes accounted for the highest abundance (around 53%), and the alpha- and gamma-gliadin amplicons with the highest number of epitopes were present in the D-subgenome. The durum wheat and tritordeum genotypes showed the lowest number of alpha- and gamma-gliadin CD epitopes. Our results allow progress in unraveling the immunogenic complexes of alpha- and gamma-gliadins and can contribute to the development of low-immunogenic varieties within precision breeding programs, by crossing or by CRISPR/Cas gene editing.https://www.frontiersin.org/articles/10.3389/fpls.2023.1171882/fullampliconalpha-gliadinsgamma-gliadinsprecision breedingwheatCRiSPR/Cas |
| spellingShingle | Miriam Marín-Sanz Francisco Barro Susana Sánchez-León Unraveling the celiac disease-related immunogenic complexes in a set of wheat and tritordeum genotypes: implications for low-gluten precision breeding in cereal crops Frontiers in Plant Science amplicon alpha-gliadins gamma-gliadins precision breeding wheat CRiSPR/Cas |
| title | Unraveling the celiac disease-related immunogenic complexes in a set of wheat and tritordeum genotypes: implications for low-gluten precision breeding in cereal crops |
| title_full | Unraveling the celiac disease-related immunogenic complexes in a set of wheat and tritordeum genotypes: implications for low-gluten precision breeding in cereal crops |
| title_fullStr | Unraveling the celiac disease-related immunogenic complexes in a set of wheat and tritordeum genotypes: implications for low-gluten precision breeding in cereal crops |
| title_full_unstemmed | Unraveling the celiac disease-related immunogenic complexes in a set of wheat and tritordeum genotypes: implications for low-gluten precision breeding in cereal crops |
| title_short | Unraveling the celiac disease-related immunogenic complexes in a set of wheat and tritordeum genotypes: implications for low-gluten precision breeding in cereal crops |
| title_sort | unraveling the celiac disease related immunogenic complexes in a set of wheat and tritordeum genotypes implications for low gluten precision breeding in cereal crops |
| topic | amplicon alpha-gliadins gamma-gliadins precision breeding wheat CRiSPR/Cas |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1171882/full |
| work_keys_str_mv | AT miriammarinsanz unravelingtheceliacdiseaserelatedimmunogeniccomplexesinasetofwheatandtritordeumgenotypesimplicationsforlowglutenprecisionbreedingincerealcrops AT franciscobarro unravelingtheceliacdiseaserelatedimmunogeniccomplexesinasetofwheatandtritordeumgenotypesimplicationsforlowglutenprecisionbreedingincerealcrops AT susanasanchezleon unravelingtheceliacdiseaserelatedimmunogeniccomplexesinasetofwheatandtritordeumgenotypesimplicationsforlowglutenprecisionbreedingincerealcrops |