Nanofibrous insulin/vildagliptin core-shell PLGA scaffold promotes diabetic wound healing
Introduction: Slow wound repair in diabetes is a serious adverse event that often results in loss of a limb or disability. An advanced and encouraging vehicle is wanted to enhance clinically applicable diabetic wound care. Nanofibrous insulin/vildagliptin core-shell biodegradable poly (lactic-co-gly...
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| Format: | Article |
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1075720/full |
| _version_ | 1797840537993084928 |
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| author | Chen-Hung Lee Dong-Yi Chen Ming-Jer Hsieh Kuo-Chun Hung Shu-Chun Huang Shu-Chun Huang Shu-Chun Huang Chia-Jung Cho Shih-Jung Liu Shih-Jung Liu |
| author_facet | Chen-Hung Lee Dong-Yi Chen Ming-Jer Hsieh Kuo-Chun Hung Shu-Chun Huang Shu-Chun Huang Shu-Chun Huang Chia-Jung Cho Shih-Jung Liu Shih-Jung Liu |
| author_sort | Chen-Hung Lee |
| collection | DOAJ |
| description | Introduction: Slow wound repair in diabetes is a serious adverse event that often results in loss of a limb or disability. An advanced and encouraging vehicle is wanted to enhance clinically applicable diabetic wound care. Nanofibrous insulin/vildagliptin core-shell biodegradable poly (lactic-co-glycolic acid) (PLGA) scaffolds to prolong the effective drug delivery of vildagliptin and insulin for the repair of diabetic wounds were prepared.Methods: To fabricate core-shell nanofibrous membranes, vildagliptin mixture with PLGA, and insulin solution were pumped via separate pumps into two differently sized capillary tubes that were coaxially electrospun.Results and Discussion: Nanofibrous core-shell scaffolds slowly released effective vildagliptin and insulin over 2 weeks in vitro migration assay and in vivo wound-healing models. Water contact angle (68.3 ± 8.5° vs. 121.4 ± 2.0°, p = 0.006) and peaked water absorbent capacity (376% ± 9% vs. 283% ± 24%, p = 0.003) of the insulin/vildagliptin core-shell nanofibrous membranes remarkably exceeded those of a control group. The insulin/vildagliptin-loaded core-shell nanofibers improved endothelial progenitor cells migration in vitro (762 ± 77 cells/mm2 vs. 424.4 ± 23 cells/mm2, p < 0.001), reduced the α-smooth muscle actin content in vivo (0.72 ± 0.23 vs. 2.07 ± 0.37, p < 0.001), and increased diabetic would recovery (1.9 ± 0.3 mm2 vs. 8.0 ± 1.4 mm2, p = 0.002). Core-shell insulin/vildagliptin-loaded nanofibers extend the drug delivery of insulin and vildagliptin and accelerate the repair of wounds associated with diabetes. |
| first_indexed | 2024-04-09T16:16:59Z |
| format | Article |
| id | doaj.art-61d09fc87ea34d55bb8cb07437522d04 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-09T16:16:59Z |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-61d09fc87ea34d55bb8cb07437522d042023-04-24T04:17:16ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-04-011110.3389/fbioe.2023.10757201075720Nanofibrous insulin/vildagliptin core-shell PLGA scaffold promotes diabetic wound healingChen-Hung Lee0Dong-Yi Chen1Ming-Jer Hsieh2Kuo-Chun Hung3Shu-Chun Huang4Shu-Chun Huang5Shu-Chun Huang6Chia-Jung Cho7Shih-Jung Liu8Shih-Jung Liu9Division of Cardiology, Department of Internal Medicine, Chang Gung Memorial Hospital-Linkou, Chang Gung University College of Medicine, Taoyuan, TaiwanDivision of Cardiology, Department of Internal Medicine, Chang Gung Memorial Hospital-Linkou, Chang Gung University College of Medicine, Taoyuan, TaiwanDivision of Cardiology, Department of Internal Medicine, Chang Gung Memorial Hospital-Linkou, Chang Gung University College of Medicine, Taoyuan, TaiwanDivision of Cardiology, Department of Internal Medicine, Chang Gung Memorial Hospital-Linkou, Chang Gung University College of Medicine, Taoyuan, TaiwanDepartment of Physical Medicine and Rehabilitation, New Taipei Municipal Tucheng Hospital, Chang Gung Memorial Hospital, New Taipei City, TaiwanDepartment of Physical Medicine & Rehabilitation, Chang Gung Memorial Hospital, Linkou, TaiwanCollege of Medicine, Chang Gung University, Taoyuan, TaiwanInstitute of Biotechnology and Chemical Engineering, I-Shou University, Kaohsiung, TaiwanDepartment of Orthopedic Surgery, Bone and Joint Research Center, Chang Gung Memorial Hospital-Linkou, Taoyuan, TaiwanDepartment of Mechanical Engineering, Chang Gung University, Taoyuan, TaiwanIntroduction: Slow wound repair in diabetes is a serious adverse event that often results in loss of a limb or disability. An advanced and encouraging vehicle is wanted to enhance clinically applicable diabetic wound care. Nanofibrous insulin/vildagliptin core-shell biodegradable poly (lactic-co-glycolic acid) (PLGA) scaffolds to prolong the effective drug delivery of vildagliptin and insulin for the repair of diabetic wounds were prepared.Methods: To fabricate core-shell nanofibrous membranes, vildagliptin mixture with PLGA, and insulin solution were pumped via separate pumps into two differently sized capillary tubes that were coaxially electrospun.Results and Discussion: Nanofibrous core-shell scaffolds slowly released effective vildagliptin and insulin over 2 weeks in vitro migration assay and in vivo wound-healing models. Water contact angle (68.3 ± 8.5° vs. 121.4 ± 2.0°, p = 0.006) and peaked water absorbent capacity (376% ± 9% vs. 283% ± 24%, p = 0.003) of the insulin/vildagliptin core-shell nanofibrous membranes remarkably exceeded those of a control group. The insulin/vildagliptin-loaded core-shell nanofibers improved endothelial progenitor cells migration in vitro (762 ± 77 cells/mm2 vs. 424.4 ± 23 cells/mm2, p < 0.001), reduced the α-smooth muscle actin content in vivo (0.72 ± 0.23 vs. 2.07 ± 0.37, p < 0.001), and increased diabetic would recovery (1.9 ± 0.3 mm2 vs. 8.0 ± 1.4 mm2, p = 0.002). Core-shell insulin/vildagliptin-loaded nanofibers extend the drug delivery of insulin and vildagliptin and accelerate the repair of wounds associated with diabetes.https://www.frontiersin.org/articles/10.3389/fbioe.2023.1075720/fullcore-shell nanofiberdiabeteswoundinsulinvildagliptin |
| spellingShingle | Chen-Hung Lee Dong-Yi Chen Ming-Jer Hsieh Kuo-Chun Hung Shu-Chun Huang Shu-Chun Huang Shu-Chun Huang Chia-Jung Cho Shih-Jung Liu Shih-Jung Liu Nanofibrous insulin/vildagliptin core-shell PLGA scaffold promotes diabetic wound healing Frontiers in Bioengineering and Biotechnology core-shell nanofiber diabetes wound insulin vildagliptin |
| title | Nanofibrous insulin/vildagliptin core-shell PLGA scaffold promotes diabetic wound healing |
| title_full | Nanofibrous insulin/vildagliptin core-shell PLGA scaffold promotes diabetic wound healing |
| title_fullStr | Nanofibrous insulin/vildagliptin core-shell PLGA scaffold promotes diabetic wound healing |
| title_full_unstemmed | Nanofibrous insulin/vildagliptin core-shell PLGA scaffold promotes diabetic wound healing |
| title_short | Nanofibrous insulin/vildagliptin core-shell PLGA scaffold promotes diabetic wound healing |
| title_sort | nanofibrous insulin vildagliptin core shell plga scaffold promotes diabetic wound healing |
| topic | core-shell nanofiber diabetes wound insulin vildagliptin |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1075720/full |
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