Cucurbitacin E inhibits cellular proliferation and induces apoptosis in melanoma by suppressing HSDL2 expression
Abstract Background Melanoma is among the most aggressive types of skin malignancy and can have an unpredictable clinical course. Exploration of novel therapeutic targets and their regulators remains essential for the prevention and treatment of melanoma. Methods HSDL2 protein levels were examined b...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2022-02-01
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| Series: | Chinese Medicine |
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| Online Access: | https://doi.org/10.1186/s13020-022-00582-y |
| _version_ | 1830405176891015168 |
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| author | Wen-Bei Liu He-Li Wang Lei Chen Biao Tang Guolin Ke Shuai Wang Yin-Qiao Sun Junting Ma Da-Lun Lyu |
| author_facet | Wen-Bei Liu He-Li Wang Lei Chen Biao Tang Guolin Ke Shuai Wang Yin-Qiao Sun Junting Ma Da-Lun Lyu |
| author_sort | Wen-Bei Liu |
| collection | DOAJ |
| description | Abstract Background Melanoma is among the most aggressive types of skin malignancy and can have an unpredictable clinical course. Exploration of novel therapeutic targets and their regulators remains essential for the prevention and treatment of melanoma. Methods HSDL2 protein levels were examined by immunohistochemistry. The roles of HSDL2 in cell proliferation and apoptosis were identified by CCK-8 and colony formation assays. The function of HSDL2 in cell apoptosis was analysed by flow cytometry. Western blotting, cell proliferation and apoptosis and a xenograft tumour model were utilized to explore the inhibitory functions and mechanisms of CuE in melanoma. Results HSDL2 is overexpressed in melanoma and promotes melanoma progression by activating the ERK and AKT pathways. CuE could inhibit the ERK and AKT pathways by decreasing HSDL2 expression; therefore, CuE could inhibit melanoma growth in vitro and in vivo. Conclusion HSDL2 may be a promising therapeutic target against melanoma, and CuE can inhibit melanoma by downregulating HSDL2 expression. |
| first_indexed | 2024-12-20T17:40:46Z |
| format | Article |
| id | doaj.art-61d8ec7d5e184a8591d9ceee734e9025 |
| institution | Directory Open Access Journal |
| issn | 1749-8546 |
| language | English |
| last_indexed | 2024-12-20T17:40:46Z |
| publishDate | 2022-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Chinese Medicine |
| spelling | doaj.art-61d8ec7d5e184a8591d9ceee734e90252022-12-21T19:31:06ZengBMCChinese Medicine1749-85462022-02-0117111110.1186/s13020-022-00582-yCucurbitacin E inhibits cellular proliferation and induces apoptosis in melanoma by suppressing HSDL2 expressionWen-Bei Liu0He-Li Wang1Lei Chen2Biao Tang3Guolin Ke4Shuai Wang5Yin-Qiao Sun6Junting Ma7Da-Lun Lyu8Department of Dermato-Venerology and Department of Burn and Plastic Surgery, The First Affiliated Hospital of Wannan Medical CollegeDepartment of Dermato-Venerology and Department of Burn and Plastic Surgery, The First Affiliated Hospital of Wannan Medical CollegeDepartment of Dermato-Venerology and Department of Burn and Plastic Surgery, The First Affiliated Hospital of Wannan Medical CollegeDepartment of Dermato-Venerology and Department of Burn and Plastic Surgery, The First Affiliated Hospital of Wannan Medical CollegeDepartment of Dermato-Venerology and Department of Burn and Plastic Surgery, The First Affiliated Hospital of Wannan Medical CollegeDepartment of Dermato-Venerology and Department of Burn and Plastic Surgery, The First Affiliated Hospital of Wannan Medical CollegeDepartment of Dermato-Venerology and Department of Burn and Plastic Surgery, The First Affiliated Hospital of Wannan Medical CollegeDepartment of Pharmacology, School of Basic Medical Sciences, Anhui Medical UniversityDepartment of Dermato-Venerology and Department of Burn and Plastic Surgery, The First Affiliated Hospital of Wannan Medical CollegeAbstract Background Melanoma is among the most aggressive types of skin malignancy and can have an unpredictable clinical course. Exploration of novel therapeutic targets and their regulators remains essential for the prevention and treatment of melanoma. Methods HSDL2 protein levels were examined by immunohistochemistry. The roles of HSDL2 in cell proliferation and apoptosis were identified by CCK-8 and colony formation assays. The function of HSDL2 in cell apoptosis was analysed by flow cytometry. Western blotting, cell proliferation and apoptosis and a xenograft tumour model were utilized to explore the inhibitory functions and mechanisms of CuE in melanoma. Results HSDL2 is overexpressed in melanoma and promotes melanoma progression by activating the ERK and AKT pathways. CuE could inhibit the ERK and AKT pathways by decreasing HSDL2 expression; therefore, CuE could inhibit melanoma growth in vitro and in vivo. Conclusion HSDL2 may be a promising therapeutic target against melanoma, and CuE can inhibit melanoma by downregulating HSDL2 expression.https://doi.org/10.1186/s13020-022-00582-yMelanomaHSDL2Cucurbitacin EERK and AKT pathwaysProliferation and apoptosis |
| spellingShingle | Wen-Bei Liu He-Li Wang Lei Chen Biao Tang Guolin Ke Shuai Wang Yin-Qiao Sun Junting Ma Da-Lun Lyu Cucurbitacin E inhibits cellular proliferation and induces apoptosis in melanoma by suppressing HSDL2 expression Chinese Medicine Melanoma HSDL2 Cucurbitacin E ERK and AKT pathways Proliferation and apoptosis |
| title | Cucurbitacin E inhibits cellular proliferation and induces apoptosis in melanoma by suppressing HSDL2 expression |
| title_full | Cucurbitacin E inhibits cellular proliferation and induces apoptosis in melanoma by suppressing HSDL2 expression |
| title_fullStr | Cucurbitacin E inhibits cellular proliferation and induces apoptosis in melanoma by suppressing HSDL2 expression |
| title_full_unstemmed | Cucurbitacin E inhibits cellular proliferation and induces apoptosis in melanoma by suppressing HSDL2 expression |
| title_short | Cucurbitacin E inhibits cellular proliferation and induces apoptosis in melanoma by suppressing HSDL2 expression |
| title_sort | cucurbitacin e inhibits cellular proliferation and induces apoptosis in melanoma by suppressing hsdl2 expression |
| topic | Melanoma HSDL2 Cucurbitacin E ERK and AKT pathways Proliferation and apoptosis |
| url | https://doi.org/10.1186/s13020-022-00582-y |
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