New insights of phenolic compounds from optimized fruit extract of Ficus auriculata
Abstract In this study, the extraction conditions extracted maximize amounts of phenolic and bioactive compounds from the fruit extract of Ficus auriculata by using optimized response surface methodology. The antioxidant capacity was evaluated through the assay of radical scavenging ability on DPPH...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-91913-w |
| _version_ | 1819122466166931456 |
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| author | M. Shahinuzzaman Parul Akhtar N. Amin Yunus Ahmed Farah Hannan Anuar H. Misran Md. Akhtaruzzaman |
| author_facet | M. Shahinuzzaman Parul Akhtar N. Amin Yunus Ahmed Farah Hannan Anuar H. Misran Md. Akhtaruzzaman |
| author_sort | M. Shahinuzzaman |
| collection | DOAJ |
| description | Abstract In this study, the extraction conditions extracted maximize amounts of phenolic and bioactive compounds from the fruit extract of Ficus auriculata by using optimized response surface methodology. The antioxidant capacity was evaluated through the assay of radical scavenging ability on DPPH and ABTS as well as reducing power assays on total phenolic content (TPC). For the extraction purpose, the ultrasonic assisted extraction technique was employed. A second-order polynomial model satisfactorily fitted to the experimental findings concerning antioxidant activity (R2 = 0.968, P < 0.0001) and total phenolic content (R2 = 0.961, P < 0.0001), indicating a significant correlation between the experimental and expected value. The highest DPPH radical scavenging activity was achieved 85.20 ± 0.96% at the optimum extraction parameters of 52.5% ethanol (v/v), 40.0 °C temperature, and 22 min extraction time. Alternatively, the highest yield of total phenolic content was found 31.65 ± 0.94 mg GAE/g DF at the optimum extraction conditions. From the LC–ESI–MS profiling of the optimized extract, 18 bioactive compounds were tentatively identified, which may regulate the antioxidant activity of fruits of F. auriculata. |
| first_indexed | 2024-12-22T06:52:54Z |
| format | Article |
| id | doaj.art-61de10acc85047f7b127c58e381a47b0 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-22T06:52:54Z |
| publishDate | 2021-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-61de10acc85047f7b127c58e381a47b02022-12-21T18:35:05ZengNature PortfolioScientific Reports2045-23222021-06-0111111410.1038/s41598-021-91913-wNew insights of phenolic compounds from optimized fruit extract of Ficus auriculataM. Shahinuzzaman0Parul Akhtar1N. Amin2Yunus Ahmed3Farah Hannan Anuar4H. Misran5Md. Akhtaruzzaman6Department of Chemical Sciences, Faculty of Science and Technology, Universiti Kebangsaaan MalaysiaDepartment of Chemistry, Chittagong University of Engineering & TechnologyInstitute of Sustainable Energy, Universiti Tenaga Nasional (@The National Energy University)Department of Chemistry, Chittagong University of Engineering & TechnologyDepartment of Chemical Sciences, Faculty of Science and Technology, Universiti Kebangsaaan MalaysiaInstitute of Sustainable Energy, Universiti Tenaga Nasional (@The National Energy University)Solar Energy Research Institute, Universiti Kebangsaan MalaysiaAbstract In this study, the extraction conditions extracted maximize amounts of phenolic and bioactive compounds from the fruit extract of Ficus auriculata by using optimized response surface methodology. The antioxidant capacity was evaluated through the assay of radical scavenging ability on DPPH and ABTS as well as reducing power assays on total phenolic content (TPC). For the extraction purpose, the ultrasonic assisted extraction technique was employed. A second-order polynomial model satisfactorily fitted to the experimental findings concerning antioxidant activity (R2 = 0.968, P < 0.0001) and total phenolic content (R2 = 0.961, P < 0.0001), indicating a significant correlation between the experimental and expected value. The highest DPPH radical scavenging activity was achieved 85.20 ± 0.96% at the optimum extraction parameters of 52.5% ethanol (v/v), 40.0 °C temperature, and 22 min extraction time. Alternatively, the highest yield of total phenolic content was found 31.65 ± 0.94 mg GAE/g DF at the optimum extraction conditions. From the LC–ESI–MS profiling of the optimized extract, 18 bioactive compounds were tentatively identified, which may regulate the antioxidant activity of fruits of F. auriculata.https://doi.org/10.1038/s41598-021-91913-w |
| spellingShingle | M. Shahinuzzaman Parul Akhtar N. Amin Yunus Ahmed Farah Hannan Anuar H. Misran Md. Akhtaruzzaman New insights of phenolic compounds from optimized fruit extract of Ficus auriculata Scientific Reports |
| title | New insights of phenolic compounds from optimized fruit extract of Ficus auriculata |
| title_full | New insights of phenolic compounds from optimized fruit extract of Ficus auriculata |
| title_fullStr | New insights of phenolic compounds from optimized fruit extract of Ficus auriculata |
| title_full_unstemmed | New insights of phenolic compounds from optimized fruit extract of Ficus auriculata |
| title_short | New insights of phenolic compounds from optimized fruit extract of Ficus auriculata |
| title_sort | new insights of phenolic compounds from optimized fruit extract of ficus auriculata |
| url | https://doi.org/10.1038/s41598-021-91913-w |
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