Comparative proteomic investigation of drought responses in foxtail millet
Abstract Background Foxtail millet (Setaria italica L. P. Beauv) has been considered as a tractable model crop in recent years due to its short growing cycle, lower amount of repetitive DNA, inbreeding nature, small diploid genome, and outstanding abiotic stress-tolerance characteristics. With moder...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-11-01
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| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12870-018-1533-9 |
| _version_ | 1818159954047008768 |
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| author | Jiaowen Pan Zhen Li Qingguo Wang Anna K. Garrell Min Liu Yanan Guan Wenqing Zhou Wei Liu |
| author_facet | Jiaowen Pan Zhen Li Qingguo Wang Anna K. Garrell Min Liu Yanan Guan Wenqing Zhou Wei Liu |
| author_sort | Jiaowen Pan |
| collection | DOAJ |
| description | Abstract Background Foxtail millet (Setaria italica L. P. Beauv) has been considered as a tractable model crop in recent years due to its short growing cycle, lower amount of repetitive DNA, inbreeding nature, small diploid genome, and outstanding abiotic stress-tolerance characteristics. With modern agriculture facing various adversities, it’s urgent to dissect the mechanisms of how foxtail millet responds and adapts to drought and stress on the proteomic-level. Results In this research, a total of 2474 differentially expressed proteins were identified by quantitative proteomic analysis after subjecting foxtail millet seedlings to drought conditions. 321 of these 2474 proteins exhibited significant expression changes, including 252 up-regulated proteins and 69 down-regulated proteins. The resulting proteins could then be divided into different categories, such as stress and defense responses, photosynthesis, carbon metabolism, ROS scavenging, protein synthesis, etc., according to Gene Ontology annotation. Proteins implicated in fatty acid and amino acid metabolism, polyamine biosynthesis, hormone metabolism, and cell wall modifications were also identified. These obtained differential proteins and their possible biological functions under drought stress all suggested that various physiological and metabolic processes might function cooperatively to configure a new dynamic homeostasis in organisms. The expression patterns of five drought-responsive proteins were further validated using western blot analysis. The qRT-PCR was also carried out to analyze the transcription levels of 21 differentially expressed proteins. The results showed large inconsistency in the variation between proteins and the corresponding mRNAs, which showed once again that post-transcriptional modification performs crucial roles in regulating gene expression. Conclusion The results offered a valuable inventory of proteins that may be involved in drought response and adaption, and provided a regulatory network of different metabolic pathways under stress stimulation. This study will illuminate the stress tolerance mechanisms of foxtail millet, and shed some light on crop germplasm breeding and innovation. |
| first_indexed | 2024-12-11T15:54:11Z |
| format | Article |
| id | doaj.art-61e4ba7dd0304a9fa6990db36830bbbf |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-11T15:54:11Z |
| publishDate | 2018-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-61e4ba7dd0304a9fa6990db36830bbbf2022-12-22T00:59:29ZengBMCBMC Plant Biology1471-22292018-11-0118111910.1186/s12870-018-1533-9Comparative proteomic investigation of drought responses in foxtail milletJiaowen Pan0Zhen Li1Qingguo Wang2Anna K. Garrell3Min Liu4Yanan Guan5Wenqing Zhou6Wei Liu7Biotechnology Research Center, Shandong Academy of Agricultural Sciences; Key Laboratory of Genetic Improvement, Ecology and Physiology of CropsBiotechnology Research Center, Shandong Academy of Agricultural Sciences; Key Laboratory of Genetic Improvement, Ecology and Physiology of CropsBiotechnology Research Center, Shandong Academy of Agricultural Sciences; Key Laboratory of Genetic Improvement, Ecology and Physiology of CropsBoragen IncShandong Agriculture and Engineering UniversityCrop Research Institute, Shandong Academy of Agricultural SciencesBoragen IncBiotechnology Research Center, Shandong Academy of Agricultural Sciences; Key Laboratory of Genetic Improvement, Ecology and Physiology of CropsAbstract Background Foxtail millet (Setaria italica L. P. Beauv) has been considered as a tractable model crop in recent years due to its short growing cycle, lower amount of repetitive DNA, inbreeding nature, small diploid genome, and outstanding abiotic stress-tolerance characteristics. With modern agriculture facing various adversities, it’s urgent to dissect the mechanisms of how foxtail millet responds and adapts to drought and stress on the proteomic-level. Results In this research, a total of 2474 differentially expressed proteins were identified by quantitative proteomic analysis after subjecting foxtail millet seedlings to drought conditions. 321 of these 2474 proteins exhibited significant expression changes, including 252 up-regulated proteins and 69 down-regulated proteins. The resulting proteins could then be divided into different categories, such as stress and defense responses, photosynthesis, carbon metabolism, ROS scavenging, protein synthesis, etc., according to Gene Ontology annotation. Proteins implicated in fatty acid and amino acid metabolism, polyamine biosynthesis, hormone metabolism, and cell wall modifications were also identified. These obtained differential proteins and their possible biological functions under drought stress all suggested that various physiological and metabolic processes might function cooperatively to configure a new dynamic homeostasis in organisms. The expression patterns of five drought-responsive proteins were further validated using western blot analysis. The qRT-PCR was also carried out to analyze the transcription levels of 21 differentially expressed proteins. The results showed large inconsistency in the variation between proteins and the corresponding mRNAs, which showed once again that post-transcriptional modification performs crucial roles in regulating gene expression. Conclusion The results offered a valuable inventory of proteins that may be involved in drought response and adaption, and provided a regulatory network of different metabolic pathways under stress stimulation. This study will illuminate the stress tolerance mechanisms of foxtail millet, and shed some light on crop germplasm breeding and innovation.http://link.springer.com/article/10.1186/s12870-018-1533-9Foxtail millet (Setaria italica L.)Drought stressComparative proteomicsExpression patternWestern blotqRT-PCR |
| spellingShingle | Jiaowen Pan Zhen Li Qingguo Wang Anna K. Garrell Min Liu Yanan Guan Wenqing Zhou Wei Liu Comparative proteomic investigation of drought responses in foxtail millet BMC Plant Biology Foxtail millet (Setaria italica L.) Drought stress Comparative proteomics Expression pattern Western blot qRT-PCR |
| title | Comparative proteomic investigation of drought responses in foxtail millet |
| title_full | Comparative proteomic investigation of drought responses in foxtail millet |
| title_fullStr | Comparative proteomic investigation of drought responses in foxtail millet |
| title_full_unstemmed | Comparative proteomic investigation of drought responses in foxtail millet |
| title_short | Comparative proteomic investigation of drought responses in foxtail millet |
| title_sort | comparative proteomic investigation of drought responses in foxtail millet |
| topic | Foxtail millet (Setaria italica L.) Drought stress Comparative proteomics Expression pattern Western blot qRT-PCR |
| url | http://link.springer.com/article/10.1186/s12870-018-1533-9 |
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