HAG Effector Evolution in Pyricularia Species and Plant Cell Death Suppression by HAG4
Seventy host-adapted gene (HAG) effector family members from Pyricularia species are found in P. oryzae and three closely related species (isolates LS and 18-2 from an unknown Pyricularia sp., P. grisea, and P. pennisetigena) that share at least eight orthologous HAG family members with P. oryzae. T...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2022-08-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-01-22-0010-R |
| _version_ | 1811338197231206400 |
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| author | Meilian Chen Nick Farmer Zhenhui Zhong Wenhui Zheng Wei Tang Yijuan Han Guodong Lu Zonghua Wang Daniel J. Ebbole |
| author_facet | Meilian Chen Nick Farmer Zhenhui Zhong Wenhui Zheng Wei Tang Yijuan Han Guodong Lu Zonghua Wang Daniel J. Ebbole |
| author_sort | Meilian Chen |
| collection | DOAJ |
| description | Seventy host-adapted gene (HAG) effector family members from Pyricularia species are found in P. oryzae and three closely related species (isolates LS and 18-2 from an unknown Pyricularia sp., P. grisea, and P. pennisetigena) that share at least eight orthologous HAG family members with P. oryzae. The genome sequence of a more distantly related species, P. penniseti, lacks HAG genes, suggesting a time frame for the origin of the gene family in the genus. In P. oryzae, HAG4 is uniquely found in the genetic lineage that contains populations adapted to Setaria and Oryza hosts. We find a nearly identical HAG4 allele in a P. grisea isolate, suggesting transfer of HAG4 from P. grisea to P. oryzae. HAG4 encodes a suppressor of plant cell death. Yeast two-hybrid screens with several HAG genes independently identify common interacting clones from a rice complementary DNA library, suggesting conservation of protein surface motifs between HAG homologs with as little as 40% protein sequence identity. HAG family orthologs have diverged rapidly and HAG15 orthologs display unusually high rates of sequence divergence compared with adjacent genes suggesting gene-specific accelerated divergence. The sequence diversity of the HAG homologs in Pyricularia species provides a resource for examining mechanisms of gene family evolution and the relationship to structural and functional evolution of HAG effector family activity. [Graphic: see text] Copyright © 2022 The Author(s). This is an open access article distributed under the CC BY 4.0 International license. |
| first_indexed | 2024-04-13T18:07:13Z |
| format | Article |
| id | doaj.art-61e4dd1e42574e0bb6aca185646bacc3 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T18:07:13Z |
| publishDate | 2022-08-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-61e4dd1e42574e0bb6aca185646bacc32022-12-22T02:36:02ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062022-08-0135869470510.1094/MPMI-01-22-0010-RHAG Effector Evolution in Pyricularia Species and Plant Cell Death Suppression by HAG4Meilian Chen0Nick Farmer1Zhenhui Zhong2Wenhui Zheng3Wei Tang4Yijuan Han5Guodong Lu6Zonghua Wang7Daniel J. Ebbole8College of Materials and Chemical Engineering, Minjiang University, Fuzhou 350108, ChinaDepartment of Plant Pathology & Microbiology, Texas A&M University, College Station, TX 77843, U.S.A.Key Laboratory of Bio-Pesticide and Chemistry-Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaKey Laboratory of Bio-Pesticide and Chemistry-Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaKey Laboratory of Bio-Pesticide and Chemistry-Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Materials and Chemical Engineering, Minjiang University, Fuzhou 350108, ChinaKey Laboratory of Bio-Pesticide and Chemistry-Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Materials and Chemical Engineering, Minjiang University, Fuzhou 350108, ChinaDepartment of Plant Pathology & Microbiology, Texas A&M University, College Station, TX 77843, U.S.A.Seventy host-adapted gene (HAG) effector family members from Pyricularia species are found in P. oryzae and three closely related species (isolates LS and 18-2 from an unknown Pyricularia sp., P. grisea, and P. pennisetigena) that share at least eight orthologous HAG family members with P. oryzae. The genome sequence of a more distantly related species, P. penniseti, lacks HAG genes, suggesting a time frame for the origin of the gene family in the genus. In P. oryzae, HAG4 is uniquely found in the genetic lineage that contains populations adapted to Setaria and Oryza hosts. We find a nearly identical HAG4 allele in a P. grisea isolate, suggesting transfer of HAG4 from P. grisea to P. oryzae. HAG4 encodes a suppressor of plant cell death. Yeast two-hybrid screens with several HAG genes independently identify common interacting clones from a rice complementary DNA library, suggesting conservation of protein surface motifs between HAG homologs with as little as 40% protein sequence identity. HAG family orthologs have diverged rapidly and HAG15 orthologs display unusually high rates of sequence divergence compared with adjacent genes suggesting gene-specific accelerated divergence. The sequence diversity of the HAG homologs in Pyricularia species provides a resource for examining mechanisms of gene family evolution and the relationship to structural and functional evolution of HAG effector family activity. [Graphic: see text] Copyright © 2022 The Author(s). This is an open access article distributed under the CC BY 4.0 International license.https://apsjournals.apsnet.org/doi/10.1094/MPMI-01-22-0010-RILR3JMJ715MagnaportheMGG_17587OsbZIP39pseudoparalog |
| spellingShingle | Meilian Chen Nick Farmer Zhenhui Zhong Wenhui Zheng Wei Tang Yijuan Han Guodong Lu Zonghua Wang Daniel J. Ebbole HAG Effector Evolution in Pyricularia Species and Plant Cell Death Suppression by HAG4 Molecular Plant-Microbe Interactions ILR3 JMJ715 Magnaporthe MGG_17587 OsbZIP39 pseudoparalog |
| title | HAG Effector Evolution in Pyricularia Species and Plant Cell Death Suppression by HAG4 |
| title_full | HAG Effector Evolution in Pyricularia Species and Plant Cell Death Suppression by HAG4 |
| title_fullStr | HAG Effector Evolution in Pyricularia Species and Plant Cell Death Suppression by HAG4 |
| title_full_unstemmed | HAG Effector Evolution in Pyricularia Species and Plant Cell Death Suppression by HAG4 |
| title_short | HAG Effector Evolution in Pyricularia Species and Plant Cell Death Suppression by HAG4 |
| title_sort | hag effector evolution in pyricularia species and plant cell death suppression by hag4 |
| topic | ILR3 JMJ715 Magnaporthe MGG_17587 OsbZIP39 pseudoparalog |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-01-22-0010-R |
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