A Sandwich-Type Electrochemical Immunosensor Using Antibody-Conjugated Pt-Doped CdTe QDs as Enzyme-Free Labels for Sensitive HER2 Detection Based on a Magnetic Framework
Tumor markers are highly sensitive and play an important role in the early diagnosis of cancer. We developed an electrochemical sandwich-type immunosensor that detects human epidermal growth factor receptor 2 (HER2). Magnetic framework (Fe3O4@ TMU-24) and AuNPs (Fe3O4@ TMU-24 -AuNPs) are utilized in...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Chemistry |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fchem.2022.881960/full |
| _version_ | 1818238616150736896 |
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| author | Hosna Ehzari Meysam Safari |
| author_facet | Hosna Ehzari Meysam Safari |
| author_sort | Hosna Ehzari |
| collection | DOAJ |
| description | Tumor markers are highly sensitive and play an important role in the early diagnosis of cancer. We developed an electrochemical sandwich-type immunosensor that detects human epidermal growth factor receptor 2 (HER2). Magnetic framework (Fe3O4@ TMU-24) and AuNPs (Fe3O4@ TMU-24 -AuNPs) are utilized in this sensing platform. In addition to their high specific surface area and excellent biocompatibility, Fe3O4@ TMU-24-AuNPs nanocomposites exhibited excellent electrocatalytic properties. The primary antibody of HER2 (Ab1) was immobilized on the surface of the Fe3O4@ TMU-24-AuNPs. In this sensing method, palatine doped to CdTe QDs (Pt: CdTe QDs) is utilized as a novel labeling signal biomolecule (secondary antibodies). Pt: CdTe QDs own good biocompatibility and excellent catalytic performance. The amperometric technique was used to achieve the quantitative determination of HER2 by using a sandwich-type electrochemical immunosensor. Under the optimum conditions, the dependency of the current signal and HER2 concentration showed a linear region from 1 pg ml−1–100 ng ml−1 with 0.175 pg ml−1 as the limit of detection. This biosensing device also showed long stability and good reproducibility, which can be used for the quantitative assay of HER2. |
| first_indexed | 2024-12-12T12:44:29Z |
| format | Article |
| id | doaj.art-61e6833ff8ec485d891cdad695014ab5 |
| institution | Directory Open Access Journal |
| issn | 2296-2646 |
| language | English |
| last_indexed | 2024-12-12T12:44:29Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Chemistry |
| spelling | doaj.art-61e6833ff8ec485d891cdad695014ab52022-12-22T00:24:08ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462022-06-011010.3389/fchem.2022.881960881960A Sandwich-Type Electrochemical Immunosensor Using Antibody-Conjugated Pt-Doped CdTe QDs as Enzyme-Free Labels for Sensitive HER2 Detection Based on a Magnetic FrameworkHosna EhzariMeysam SafariTumor markers are highly sensitive and play an important role in the early diagnosis of cancer. We developed an electrochemical sandwich-type immunosensor that detects human epidermal growth factor receptor 2 (HER2). Magnetic framework (Fe3O4@ TMU-24) and AuNPs (Fe3O4@ TMU-24 -AuNPs) are utilized in this sensing platform. In addition to their high specific surface area and excellent biocompatibility, Fe3O4@ TMU-24-AuNPs nanocomposites exhibited excellent electrocatalytic properties. The primary antibody of HER2 (Ab1) was immobilized on the surface of the Fe3O4@ TMU-24-AuNPs. In this sensing method, palatine doped to CdTe QDs (Pt: CdTe QDs) is utilized as a novel labeling signal biomolecule (secondary antibodies). Pt: CdTe QDs own good biocompatibility and excellent catalytic performance. The amperometric technique was used to achieve the quantitative determination of HER2 by using a sandwich-type electrochemical immunosensor. Under the optimum conditions, the dependency of the current signal and HER2 concentration showed a linear region from 1 pg ml−1–100 ng ml−1 with 0.175 pg ml−1 as the limit of detection. This biosensing device also showed long stability and good reproducibility, which can be used for the quantitative assay of HER2.https://www.frontiersin.org/articles/10.3389/fchem.2022.881960/fullhuman epidermal growth factor receptor 2sandwich-type electrochemical immunosensormetal–organic frameworksquantum dotsmagnetic framework |
| spellingShingle | Hosna Ehzari Meysam Safari A Sandwich-Type Electrochemical Immunosensor Using Antibody-Conjugated Pt-Doped CdTe QDs as Enzyme-Free Labels for Sensitive HER2 Detection Based on a Magnetic Framework Frontiers in Chemistry human epidermal growth factor receptor 2 sandwich-type electrochemical immunosensor metal–organic frameworks quantum dots magnetic framework |
| title | A Sandwich-Type Electrochemical Immunosensor Using Antibody-Conjugated Pt-Doped CdTe QDs as Enzyme-Free Labels for Sensitive HER2 Detection Based on a Magnetic Framework |
| title_full | A Sandwich-Type Electrochemical Immunosensor Using Antibody-Conjugated Pt-Doped CdTe QDs as Enzyme-Free Labels for Sensitive HER2 Detection Based on a Magnetic Framework |
| title_fullStr | A Sandwich-Type Electrochemical Immunosensor Using Antibody-Conjugated Pt-Doped CdTe QDs as Enzyme-Free Labels for Sensitive HER2 Detection Based on a Magnetic Framework |
| title_full_unstemmed | A Sandwich-Type Electrochemical Immunosensor Using Antibody-Conjugated Pt-Doped CdTe QDs as Enzyme-Free Labels for Sensitive HER2 Detection Based on a Magnetic Framework |
| title_short | A Sandwich-Type Electrochemical Immunosensor Using Antibody-Conjugated Pt-Doped CdTe QDs as Enzyme-Free Labels for Sensitive HER2 Detection Based on a Magnetic Framework |
| title_sort | sandwich type electrochemical immunosensor using antibody conjugated pt doped cdte qds as enzyme free labels for sensitive her2 detection based on a magnetic framework |
| topic | human epidermal growth factor receptor 2 sandwich-type electrochemical immunosensor metal–organic frameworks quantum dots magnetic framework |
| url | https://www.frontiersin.org/articles/10.3389/fchem.2022.881960/full |
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