The small protein CydX is required for function of cytochrome bd oxidase in Brucella abortus
A large number of hypothetical genes potentially encoding small proteins of unknown function are annotated in the Brucella abortus genome. Individual deletion of 30 of these genes identified four mutants, in BAB1_0355, BAB2¬_0726, BAB2_0470 and BAB2_0450, that were highly attenuated for inf...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2012-04-01
|
| Series: | Frontiers in Cellular and Infection Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fcimb.2012.00047/full |
| _version_ | 1811283989734883328 |
|---|---|
| author | Yao-Hui eSun Maarten F de Jong Andreas B den Hartigh Christelle M Roux Hortensia G Rolan Renee M Tsolis |
| author_facet | Yao-Hui eSun Maarten F de Jong Andreas B den Hartigh Christelle M Roux Hortensia G Rolan Renee M Tsolis |
| author_sort | Yao-Hui eSun |
| collection | DOAJ |
| description | A large number of hypothetical genes potentially encoding small proteins of unknown function are annotated in the Brucella abortus genome. Individual deletion of 30 of these genes identified four mutants, in BAB1_0355, BAB2¬_0726, BAB2_0470 and BAB2_0450, that were highly attenuated for infection. BAB2_0726, a YbgT-family protein located at the 3’ end of the cydAB genes encoding cytochrome bd ubiquinal oxidase, was designated cydX. A B. abortus cydX mutant lacked cytochrome bd oxidase activity, as shown by increased sensitivity to H2O2, decreased acid tolerance and increased resistance to killing by respiratory inhibitors. The C terminus, but not the N terminus, of CydX was located in the periplasm, suggesting that CydX is an integral cytoplasmic membrane protein. Phenotypic analysis of the cydX mutant therefore suggested that CydX is required for full function of cytochrome bd oxidase, possibly via regulation of its assembly or activity. |
| first_indexed | 2024-04-13T02:21:54Z |
| format | Article |
| id | doaj.art-61e73b084eb84cf2ac777c6292ad54f9 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-04-13T02:21:54Z |
| publishDate | 2012-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-61e73b084eb84cf2ac777c6292ad54f92022-12-22T03:06:55ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882012-04-01210.3389/fcimb.2012.0004715772The small protein CydX is required for function of cytochrome bd oxidase in Brucella abortusYao-Hui eSun0Maarten F de Jong1Andreas B den Hartigh2Christelle M Roux3Hortensia G Rolan4Renee M Tsolis5University of California-DavisUniversity of California-DavisUniversity of California-DavisUniversity of California-DavisUniversity of California-DavisUniversity of California-DavisA large number of hypothetical genes potentially encoding small proteins of unknown function are annotated in the Brucella abortus genome. Individual deletion of 30 of these genes identified four mutants, in BAB1_0355, BAB2¬_0726, BAB2_0470 and BAB2_0450, that were highly attenuated for infection. BAB2_0726, a YbgT-family protein located at the 3’ end of the cydAB genes encoding cytochrome bd ubiquinal oxidase, was designated cydX. A B. abortus cydX mutant lacked cytochrome bd oxidase activity, as shown by increased sensitivity to H2O2, decreased acid tolerance and increased resistance to killing by respiratory inhibitors. The C terminus, but not the N terminus, of CydX was located in the periplasm, suggesting that CydX is an integral cytoplasmic membrane protein. Phenotypic analysis of the cydX mutant therefore suggested that CydX is required for full function of cytochrome bd oxidase, possibly via regulation of its assembly or activity.http://journal.frontiersin.org/Journal/10.3389/fcimb.2012.00047/fullpeptidecytochrome oxidasemutant screenterminal electron acceptor |
| spellingShingle | Yao-Hui eSun Maarten F de Jong Andreas B den Hartigh Christelle M Roux Hortensia G Rolan Renee M Tsolis The small protein CydX is required for function of cytochrome bd oxidase in Brucella abortus Frontiers in Cellular and Infection Microbiology peptide cytochrome oxidase mutant screen terminal electron acceptor |
| title | The small protein CydX is required for function of cytochrome bd oxidase in Brucella abortus |
| title_full | The small protein CydX is required for function of cytochrome bd oxidase in Brucella abortus |
| title_fullStr | The small protein CydX is required for function of cytochrome bd oxidase in Brucella abortus |
| title_full_unstemmed | The small protein CydX is required for function of cytochrome bd oxidase in Brucella abortus |
| title_short | The small protein CydX is required for function of cytochrome bd oxidase in Brucella abortus |
| title_sort | small protein cydx is required for function of cytochrome bd oxidase in brucella abortus |
| topic | peptide cytochrome oxidase mutant screen terminal electron acceptor |
| url | http://journal.frontiersin.org/Journal/10.3389/fcimb.2012.00047/full |
| work_keys_str_mv | AT yaohuiesun thesmallproteincydxisrequiredforfunctionofcytochromebdoxidaseinbrucellaabortus AT maartenfdejong thesmallproteincydxisrequiredforfunctionofcytochromebdoxidaseinbrucellaabortus AT andreasbdenhartigh thesmallproteincydxisrequiredforfunctionofcytochromebdoxidaseinbrucellaabortus AT christellemroux thesmallproteincydxisrequiredforfunctionofcytochromebdoxidaseinbrucellaabortus AT hortensiagrolan thesmallproteincydxisrequiredforfunctionofcytochromebdoxidaseinbrucellaabortus AT reneemtsolis thesmallproteincydxisrequiredforfunctionofcytochromebdoxidaseinbrucellaabortus AT yaohuiesun smallproteincydxisrequiredforfunctionofcytochromebdoxidaseinbrucellaabortus AT maartenfdejong smallproteincydxisrequiredforfunctionofcytochromebdoxidaseinbrucellaabortus AT andreasbdenhartigh smallproteincydxisrequiredforfunctionofcytochromebdoxidaseinbrucellaabortus AT christellemroux smallproteincydxisrequiredforfunctionofcytochromebdoxidaseinbrucellaabortus AT hortensiagrolan smallproteincydxisrequiredforfunctionofcytochromebdoxidaseinbrucellaabortus AT reneemtsolis smallproteincydxisrequiredforfunctionofcytochromebdoxidaseinbrucellaabortus |