DNA and nanophotonics: original methodological approach
The aim of the present work is a spectroscopic and thermodynamic study of DNA catalytic properties in the following processes: a) redox; b) formation of interstrand crosslinks; c) performing of photodynamic effects; d) nanoscale resonance radiationless electron excitation energy transfer. The most a...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2014-10-01
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| Series: | Nanotechnology Reviews |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/ntrev-2014-0021 |
| _version_ | 1818580835570286592 |
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| author | Bregadze Vasil G. Giorgadze Tamar G. Melikishvili Zaza G. |
| author_facet | Bregadze Vasil G. Giorgadze Tamar G. Melikishvili Zaza G. |
| author_sort | Bregadze Vasil G. |
| collection | DOAJ |
| description | The aim of the present work is a spectroscopic and thermodynamic study of DNA catalytic properties in the following processes: a) redox; b) formation of interstrand crosslinks; c) performing of photodynamic effects; d) nanoscale resonance radiationless electron excitation energy transfer. The most attention is paid to the latter, as it is truly nanoscale method in its origin. The nanoscale method of laser-induced fluorescence resonance energy transfer (FRET) to donor (acridine orange)-acceptor (ethidium bromide) intercalator pair for quantitative and qualitative study of stability quality DNA double helix in solution in real time is used. The FRET method allows to estimate the concentration of double helix areas with high quality stability applicable for intercalation in DNA after it is subjected to stress effect. It gives the opportunity to compare various types of DNAs with 1) different origins; 2) various degrees of damage; 3) being in various functional states. An alternative model and mechanisms of photodynamic effect on DNA in solutions are proposed. They are based on photoenergy degradation in solutions. The energy activates electrolytic dissociation of water molecules on H3O+ and OH- and acts as a catalyst for hydrolysis reactions of phosphordiester and glycoside linkages. |
| first_indexed | 2024-12-16T07:23:55Z |
| format | Article |
| id | doaj.art-61e99ac529fe49dc94be33b966d3b133 |
| institution | Directory Open Access Journal |
| issn | 2191-9089 2191-9097 |
| language | English |
| last_indexed | 2024-12-16T07:23:55Z |
| publishDate | 2014-10-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Nanotechnology Reviews |
| spelling | doaj.art-61e99ac529fe49dc94be33b966d3b1332022-12-21T22:39:35ZengDe GruyterNanotechnology Reviews2191-90892191-90972014-10-013544546510.1515/ntrev-2014-0021DNA and nanophotonics: original methodological approachBregadze Vasil G.0Giorgadze Tamar G.1Melikishvili Zaza G.2Andronikashvili Institute of Physics, Department of Biological System Physics, Javakhishvili Tbilisi State University, Tbilisi, GeorgiaAndronikashvili Institute of Physics, Department of Biological System Physics, Javakhishvili Tbilisi State University, Tbilisi, GeorgiaChavchanidze Institute of Cybernetics, Department of Coherent Optics and Electronics, Georgian Technical University, Tbilisi, GeorgiaThe aim of the present work is a spectroscopic and thermodynamic study of DNA catalytic properties in the following processes: a) redox; b) formation of interstrand crosslinks; c) performing of photodynamic effects; d) nanoscale resonance radiationless electron excitation energy transfer. The most attention is paid to the latter, as it is truly nanoscale method in its origin. The nanoscale method of laser-induced fluorescence resonance energy transfer (FRET) to donor (acridine orange)-acceptor (ethidium bromide) intercalator pair for quantitative and qualitative study of stability quality DNA double helix in solution in real time is used. The FRET method allows to estimate the concentration of double helix areas with high quality stability applicable for intercalation in DNA after it is subjected to stress effect. It gives the opportunity to compare various types of DNAs with 1) different origins; 2) various degrees of damage; 3) being in various functional states. An alternative model and mechanisms of photodynamic effect on DNA in solutions are proposed. They are based on photoenergy degradation in solutions. The energy activates electrolytic dissociation of water molecules on H3O+ and OH- and acts as a catalyst for hydrolysis reactions of phosphordiester and glycoside linkages.https://doi.org/10.1515/ntrev-2014-0021dnananophotonicsnanoscale fluorescent probing of stressed dnaphotodynamic effectresonance energy transfer |
| spellingShingle | Bregadze Vasil G. Giorgadze Tamar G. Melikishvili Zaza G. DNA and nanophotonics: original methodological approach Nanotechnology Reviews dna nanophotonics nanoscale fluorescent probing of stressed dna photodynamic effect resonance energy transfer |
| title | DNA and nanophotonics: original methodological approach |
| title_full | DNA and nanophotonics: original methodological approach |
| title_fullStr | DNA and nanophotonics: original methodological approach |
| title_full_unstemmed | DNA and nanophotonics: original methodological approach |
| title_short | DNA and nanophotonics: original methodological approach |
| title_sort | dna and nanophotonics original methodological approach |
| topic | dna nanophotonics nanoscale fluorescent probing of stressed dna photodynamic effect resonance energy transfer |
| url | https://doi.org/10.1515/ntrev-2014-0021 |
| work_keys_str_mv | AT bregadzevasilg dnaandnanophotonicsoriginalmethodologicalapproach AT giorgadzetamarg dnaandnanophotonicsoriginalmethodologicalapproach AT melikishvilizazag dnaandnanophotonicsoriginalmethodologicalapproach |