Aquaporin-8 promotes human dermal fibroblasts to counteract hydrogen peroxide-induced oxidative damage: A novel target for management of skin aging
The skin is subjected to various external factors that contribute to aging including oxidative stress from hydrogen peroxide (H2O2). This study investigated the distribution of aquaporin-8 (AQP8), a protein that transports H2O2 across biological membranes, in skin cells, and its effects in mitigatin...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2024-02-01
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| Series: | Open Life Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/biol-2022-0828 |
| _version_ | 1797303242178166784 |
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| author | Liu Shu-Hsiang Lin Wei-Chun Liao En-Chih Lin Yung-Feng Wang Ching-Shuen Lee Sheng-Yang Pei Dee Hsu Chun-Hsien |
| author_facet | Liu Shu-Hsiang Lin Wei-Chun Liao En-Chih Lin Yung-Feng Wang Ching-Shuen Lee Sheng-Yang Pei Dee Hsu Chun-Hsien |
| author_sort | Liu Shu-Hsiang |
| collection | DOAJ |
| description | The skin is subjected to various external factors that contribute to aging including oxidative stress from hydrogen peroxide (H2O2). This study investigated the distribution of aquaporin-8 (AQP8), a protein that transports H2O2 across biological membranes, in skin cells, and its effects in mitigating H2O2-induced oxidative damage. Human dermal fibroblasts were treated with increasing concentrations of H2O2 to evaluate oxidative damage. Cell viability, reactive oxygen species (ROS) generation, and the expression of specific genes associated with skin aging (IL-10, FPR2, COL1A1, KRT19, and Aggrecan) were evaluated and AQP8 expression was assessed via quantitative polymerase chain reaction and western blotting. Small-interfering RNA was used to silence the AQP8 gene and evaluate its significance. The results show that H2O2 treatment reduces cell viability and increases ROS generation, leading to oxidative damage that affects the expression of target molecules. Interestingly, H2O2-treated cells exhibit high levels of AQP8 expression and gene silencing of AQP8 reverses high levels of ROS and low levels of COL1A1, KRT19, and Aggrecan expression in stressed cells, indicating that AQP8 plays a vital role in preventing oxidative damage and consequent aging. In conclusion, AQP8 is upregulated in human dermal fibroblasts during H2O2-induced oxidative stress and may help prevent oxidative damage and aging. These findings suggest that AQP8 could be a potential therapeutic target for skin aging. Further research is necessary to explore the feasibility of using AQP8 as a preventive or therapeutic strategy for maintaining skin health. |
| first_indexed | 2024-03-07T23:49:05Z |
| format | Article |
| id | doaj.art-61ec01022fcf47e5baaa6c6a6acad2eb |
| institution | Directory Open Access Journal |
| issn | 2391-5412 |
| language | English |
| last_indexed | 2024-03-07T23:49:05Z |
| publishDate | 2024-02-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Life Sciences |
| spelling | doaj.art-61ec01022fcf47e5baaa6c6a6acad2eb2024-02-19T09:01:18ZengDe GruyterOpen Life Sciences2391-54122024-02-011916657310.1515/biol-2022-0828Aquaporin-8 promotes human dermal fibroblasts to counteract hydrogen peroxide-induced oxidative damage: A novel target for management of skin agingLiu Shu-Hsiang0Lin Wei-Chun1Liao En-Chih2Lin Yung-Feng3Wang Ching-Shuen4Lee Sheng-Yang5Pei Dee6Hsu Chun-Hsien7School of Nursing, College of Nursing, National Taipei University of Nursing and Health Sciences, Taipei, TaiwanSchool of Dental Technology, College of Oral Medicine, Taipei Medical University, Taipei, TaiwanDepartment of Medicine, MacKay Medical College, New Taipei, TaiwanSchool of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University, Taipei, TaiwanSchool of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, TaiwanSchool of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, TaiwanDepartment of Internal Medicine, Division of Endocrinology and Metabolism, Fu Jen Catholic University Hospital, New Taipei, TaiwanDepartment of Family Medicine, Taipei City Hospital, Heping Fuyou Branch, No. 12, Fuzhou St., Zhongzheng Dist., Taipei City 100, Taiwan (R.O.C.)The skin is subjected to various external factors that contribute to aging including oxidative stress from hydrogen peroxide (H2O2). This study investigated the distribution of aquaporin-8 (AQP8), a protein that transports H2O2 across biological membranes, in skin cells, and its effects in mitigating H2O2-induced oxidative damage. Human dermal fibroblasts were treated with increasing concentrations of H2O2 to evaluate oxidative damage. Cell viability, reactive oxygen species (ROS) generation, and the expression of specific genes associated with skin aging (IL-10, FPR2, COL1A1, KRT19, and Aggrecan) were evaluated and AQP8 expression was assessed via quantitative polymerase chain reaction and western blotting. Small-interfering RNA was used to silence the AQP8 gene and evaluate its significance. The results show that H2O2 treatment reduces cell viability and increases ROS generation, leading to oxidative damage that affects the expression of target molecules. Interestingly, H2O2-treated cells exhibit high levels of AQP8 expression and gene silencing of AQP8 reverses high levels of ROS and low levels of COL1A1, KRT19, and Aggrecan expression in stressed cells, indicating that AQP8 plays a vital role in preventing oxidative damage and consequent aging. In conclusion, AQP8 is upregulated in human dermal fibroblasts during H2O2-induced oxidative stress and may help prevent oxidative damage and aging. These findings suggest that AQP8 could be a potential therapeutic target for skin aging. Further research is necessary to explore the feasibility of using AQP8 as a preventive or therapeutic strategy for maintaining skin health.https://doi.org/10.1515/biol-2022-0828aquaporin-8dermal fibroblastshydrogen peroxideoxidative stressaging |
| spellingShingle | Liu Shu-Hsiang Lin Wei-Chun Liao En-Chih Lin Yung-Feng Wang Ching-Shuen Lee Sheng-Yang Pei Dee Hsu Chun-Hsien Aquaporin-8 promotes human dermal fibroblasts to counteract hydrogen peroxide-induced oxidative damage: A novel target for management of skin aging Open Life Sciences aquaporin-8 dermal fibroblasts hydrogen peroxide oxidative stress aging |
| title | Aquaporin-8 promotes human dermal fibroblasts to counteract hydrogen peroxide-induced oxidative damage: A novel target for management of skin aging |
| title_full | Aquaporin-8 promotes human dermal fibroblasts to counteract hydrogen peroxide-induced oxidative damage: A novel target for management of skin aging |
| title_fullStr | Aquaporin-8 promotes human dermal fibroblasts to counteract hydrogen peroxide-induced oxidative damage: A novel target for management of skin aging |
| title_full_unstemmed | Aquaporin-8 promotes human dermal fibroblasts to counteract hydrogen peroxide-induced oxidative damage: A novel target for management of skin aging |
| title_short | Aquaporin-8 promotes human dermal fibroblasts to counteract hydrogen peroxide-induced oxidative damage: A novel target for management of skin aging |
| title_sort | aquaporin 8 promotes human dermal fibroblasts to counteract hydrogen peroxide induced oxidative damage a novel target for management of skin aging |
| topic | aquaporin-8 dermal fibroblasts hydrogen peroxide oxidative stress aging |
| url | https://doi.org/10.1515/biol-2022-0828 |
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