Occurrence of Antibiotic Resistance in the Mediterranean Sea
Seawater could be considered a reservoir of antibiotic-resistant bacteria and antibiotic resistance genes. In this communication, we evaluated the presence of bacterial strains in seawater collected from different coasts of Sicily by combining microbiological and molecular methods. Specifically, we...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-03-01
|
| Series: | Antibiotics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6382/11/3/332 |
| _version_ | 1797473211515928576 |
|---|---|
| author | Delia Gambino Dario Savoca Arianna Sucato Valeria Gargano Antonino Gentile Licia Pantano Domenico Vicari Rosa Alduina |
| author_facet | Delia Gambino Dario Savoca Arianna Sucato Valeria Gargano Antonino Gentile Licia Pantano Domenico Vicari Rosa Alduina |
| author_sort | Delia Gambino |
| collection | DOAJ |
| description | Seawater could be considered a reservoir of antibiotic-resistant bacteria and antibiotic resistance genes. In this communication, we evaluated the presence of bacterial strains in seawater collected from different coasts of Sicily by combining microbiological and molecular methods. Specifically, we isolated viable bacteria that were tested for their antibiotic resistance profile and detected both antibiotic and heavy metal resistance genes. Both antibiotic-resistant Gram-negative bacteria, <i>Vibrio</i> and <i>Aeromonas</i>, and specific antibiotic resistance genes were found in the seawater samples. Alarming levels of resistance were determined towards cefazolin, streptomycin, amoxicillin/clavulanic acid, ceftriaxone, and sulfamethoxazole/trimethoprim, and mainly genes conferring resistance to β-lactamic and sulfonamide antibiotics were detected. This survey, on the one hand, presents a picture of the actual situation, showing the pollution status of the Tyrrhenian coast of Sicily, and, on the other hand, can be considered as a baseline to be used as a reference time for future analysis. |
| first_indexed | 2024-03-09T20:12:35Z |
| format | Article |
| id | doaj.art-61f1a99777eb4d17b3bf0a0e13b951d4 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T20:12:35Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-61f1a99777eb4d17b3bf0a0e13b951d42023-11-24T00:10:48ZengMDPI AGAntibiotics2079-63822022-03-0111333210.3390/antibiotics11030332Occurrence of Antibiotic Resistance in the Mediterranean SeaDelia Gambino0Dario Savoca1Arianna Sucato2Valeria Gargano3Antonino Gentile4Licia Pantano5Domenico Vicari6Rosa Alduina7Istituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, 90129 Palermo, ItalyDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, 90028 Palermo, ItalyDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, 90028 Palermo, ItalyIstituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, 90129 Palermo, ItalyIstituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, 90129 Palermo, ItalyIstituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, 90129 Palermo, ItalyIstituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, 90129 Palermo, ItalyDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, 90028 Palermo, ItalySeawater could be considered a reservoir of antibiotic-resistant bacteria and antibiotic resistance genes. In this communication, we evaluated the presence of bacterial strains in seawater collected from different coasts of Sicily by combining microbiological and molecular methods. Specifically, we isolated viable bacteria that were tested for their antibiotic resistance profile and detected both antibiotic and heavy metal resistance genes. Both antibiotic-resistant Gram-negative bacteria, <i>Vibrio</i> and <i>Aeromonas</i>, and specific antibiotic resistance genes were found in the seawater samples. Alarming levels of resistance were determined towards cefazolin, streptomycin, amoxicillin/clavulanic acid, ceftriaxone, and sulfamethoxazole/trimethoprim, and mainly genes conferring resistance to β-lactamic and sulfonamide antibiotics were detected. This survey, on the one hand, presents a picture of the actual situation, showing the pollution status of the Tyrrhenian coast of Sicily, and, on the other hand, can be considered as a baseline to be used as a reference time for future analysis.https://www.mdpi.com/2079-6382/11/3/332antibiotic-resistant bacteriaantibiotic resistance genes<i>int1</i><i>bla</i><sub>TEM</sub><i>sul</i>IIheavy metal resistance genes |
| spellingShingle | Delia Gambino Dario Savoca Arianna Sucato Valeria Gargano Antonino Gentile Licia Pantano Domenico Vicari Rosa Alduina Occurrence of Antibiotic Resistance in the Mediterranean Sea Antibiotics antibiotic-resistant bacteria antibiotic resistance genes <i>int1</i> <i>bla</i><sub>TEM</sub> <i>sul</i>II heavy metal resistance genes |
| title | Occurrence of Antibiotic Resistance in the Mediterranean Sea |
| title_full | Occurrence of Antibiotic Resistance in the Mediterranean Sea |
| title_fullStr | Occurrence of Antibiotic Resistance in the Mediterranean Sea |
| title_full_unstemmed | Occurrence of Antibiotic Resistance in the Mediterranean Sea |
| title_short | Occurrence of Antibiotic Resistance in the Mediterranean Sea |
| title_sort | occurrence of antibiotic resistance in the mediterranean sea |
| topic | antibiotic-resistant bacteria antibiotic resistance genes <i>int1</i> <i>bla</i><sub>TEM</sub> <i>sul</i>II heavy metal resistance genes |
| url | https://www.mdpi.com/2079-6382/11/3/332 |
| work_keys_str_mv | AT deliagambino occurrenceofantibioticresistanceinthemediterraneansea AT dariosavoca occurrenceofantibioticresistanceinthemediterraneansea AT ariannasucato occurrenceofantibioticresistanceinthemediterraneansea AT valeriagargano occurrenceofantibioticresistanceinthemediterraneansea AT antoninogentile occurrenceofantibioticresistanceinthemediterraneansea AT liciapantano occurrenceofantibioticresistanceinthemediterraneansea AT domenicovicari occurrenceofantibioticresistanceinthemediterraneansea AT rosaalduina occurrenceofantibioticresistanceinthemediterraneansea |