An exopolysaccharide-producing novel Agrobacterium pusense strain JAS1 isolated from snake plant enhances plant growth and soil water retention
Abstract A peculiar bacterial growth was very often noticed in leaf-initiated tissue cultures of Sansevieria trifasciata, a succulent belonging to the Asparagaceae family. The isolate left trails of some highly viscous material on the walls of the suspension vessels or developed a thick overlay on s...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-12-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-25225-y |
| _version_ | 1811189007879503872 |
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| author | Jaspreet Kaur Gaurav Mudgal Kartar Chand Gajendra B. Singh Kahkashan Perveen Najat A. Bukhari Sandip Debnath Thotegowdanapalya C. Mohan Rajulu Charukesi Gaurav Singh |
| author_facet | Jaspreet Kaur Gaurav Mudgal Kartar Chand Gajendra B. Singh Kahkashan Perveen Najat A. Bukhari Sandip Debnath Thotegowdanapalya C. Mohan Rajulu Charukesi Gaurav Singh |
| author_sort | Jaspreet Kaur |
| collection | DOAJ |
| description | Abstract A peculiar bacterial growth was very often noticed in leaf-initiated tissue cultures of Sansevieria trifasciata, a succulent belonging to the Asparagaceae family. The isolate left trails of some highly viscous material on the walls of the suspension vessels or developed a thick overlay on semisolid media without adversities in plant growth. FTIR identified this substance to be an extracellular polysaccharide. Various morphological, biochemical tests, and molecular analyses using 16S rRNA, atpD, and recA genes characterized this isolate JAS1 as a novel strain of Agrobacterium pusense. Its mucoidal growth over Murashige and Skoog media yielded enormous exopolysaccharide (7252 mg l−1), while in nutrient agar it only developed fast-growing swarms. As a qualifying plant growth-promoting bacteria, it produces significant indole-3-acetic acid (86.95 mg l−1), gibberellic acid (172.98 mg l−1), ammonia (42.66 µmol ml−1). Besides, it produces siderophores, 1-aminocyclopropane-1-carboxylic acid deaminase, fixes nitrogen, forms biofilms, and productively solubilizes soil inorganic phosphates, and zinc. Under various treatments with JAS1, wheat and chickpea resulted in significantly enhanced shoot and root growth parameters. PGP effects of JAS1 positively enhanced plants’ physiological growth parameters reflecting significant increments in overall chlorophyll, carotenoids, proline, phenols, flavonoids, and sugar contents. In addition, the isolated strain maintained both plant and soil health under an intermittent soil drying regime, probably by both its PGP and EPS production attributes, respectively. |
| first_indexed | 2024-04-11T14:27:50Z |
| format | Article |
| id | doaj.art-61f9f43532ac45619e7b72b73ac99c5a |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T14:27:50Z |
| publishDate | 2022-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-61f9f43532ac45619e7b72b73ac99c5a2022-12-22T04:18:47ZengNature PortfolioScientific Reports2045-23222022-12-0112113010.1038/s41598-022-25225-yAn exopolysaccharide-producing novel Agrobacterium pusense strain JAS1 isolated from snake plant enhances plant growth and soil water retentionJaspreet Kaur0Gaurav Mudgal1Kartar Chand2Gajendra B. Singh3Kahkashan Perveen4Najat A. Bukhari5Sandip Debnath6Thotegowdanapalya C. Mohan7Rajulu Charukesi8Gaurav Singh9University Institute of Biotechnology, Chandigarh UniversityUniversity Institute of Biotechnology, Chandigarh UniversityUniversity Institute of Biotechnology, Chandigarh UniversityUniversity Institute of Biotechnology, Chandigarh UniversityDepartment of Botany and Microbiology, College of Science, King Saud UniversityDepartment of Botany and Microbiology, College of Science, King Saud UniversityDepartment of Genetics and Plant Breeding, Palli Siksha Bhavana (Institute of Agriculture), Visva-Bharati UniversityDepartment of Biotechnology and Bioinformatics, School of Life Sciences, JSS Academy of Higher Education and ResearchDepartment of Biotechnology and Bioinformatics, School of Life Sciences, JSS Academy of Higher Education and ResearchStress Signaling to the Nucleus, CNRS-Institute of Molecular Biology of PlantsAbstract A peculiar bacterial growth was very often noticed in leaf-initiated tissue cultures of Sansevieria trifasciata, a succulent belonging to the Asparagaceae family. The isolate left trails of some highly viscous material on the walls of the suspension vessels or developed a thick overlay on semisolid media without adversities in plant growth. FTIR identified this substance to be an extracellular polysaccharide. Various morphological, biochemical tests, and molecular analyses using 16S rRNA, atpD, and recA genes characterized this isolate JAS1 as a novel strain of Agrobacterium pusense. Its mucoidal growth over Murashige and Skoog media yielded enormous exopolysaccharide (7252 mg l−1), while in nutrient agar it only developed fast-growing swarms. As a qualifying plant growth-promoting bacteria, it produces significant indole-3-acetic acid (86.95 mg l−1), gibberellic acid (172.98 mg l−1), ammonia (42.66 µmol ml−1). Besides, it produces siderophores, 1-aminocyclopropane-1-carboxylic acid deaminase, fixes nitrogen, forms biofilms, and productively solubilizes soil inorganic phosphates, and zinc. Under various treatments with JAS1, wheat and chickpea resulted in significantly enhanced shoot and root growth parameters. PGP effects of JAS1 positively enhanced plants’ physiological growth parameters reflecting significant increments in overall chlorophyll, carotenoids, proline, phenols, flavonoids, and sugar contents. In addition, the isolated strain maintained both plant and soil health under an intermittent soil drying regime, probably by both its PGP and EPS production attributes, respectively.https://doi.org/10.1038/s41598-022-25225-y |
| spellingShingle | Jaspreet Kaur Gaurav Mudgal Kartar Chand Gajendra B. Singh Kahkashan Perveen Najat A. Bukhari Sandip Debnath Thotegowdanapalya C. Mohan Rajulu Charukesi Gaurav Singh An exopolysaccharide-producing novel Agrobacterium pusense strain JAS1 isolated from snake plant enhances plant growth and soil water retention Scientific Reports |
| title | An exopolysaccharide-producing novel Agrobacterium pusense strain JAS1 isolated from snake plant enhances plant growth and soil water retention |
| title_full | An exopolysaccharide-producing novel Agrobacterium pusense strain JAS1 isolated from snake plant enhances plant growth and soil water retention |
| title_fullStr | An exopolysaccharide-producing novel Agrobacterium pusense strain JAS1 isolated from snake plant enhances plant growth and soil water retention |
| title_full_unstemmed | An exopolysaccharide-producing novel Agrobacterium pusense strain JAS1 isolated from snake plant enhances plant growth and soil water retention |
| title_short | An exopolysaccharide-producing novel Agrobacterium pusense strain JAS1 isolated from snake plant enhances plant growth and soil water retention |
| title_sort | exopolysaccharide producing novel agrobacterium pusense strain jas1 isolated from snake plant enhances plant growth and soil water retention |
| url | https://doi.org/10.1038/s41598-022-25225-y |
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