Distinct Cell Transcriptomic Landscapes Upon Henipavirus Infections
Hendra virus (HeV) and Cedar virus (CedV) are henipaviruses, which fall into the Paramyxoviridae family of single-stranded, negative-sense RNA viruses. HeV is classified as a Biosafety Level-4 (BSL-4) agent, as it is highly pathogenic and is often fatal to humans. To date, no HeV prevention or treat...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-05-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2020.00986/full |
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| author | Mingyue Chen Mingyue Chen Mingyue Chen Mary Tachedjian Glenn A. Marsh Jie Cui Jie Cui Lin-Fa Wang Lin-Fa Wang |
| author_facet | Mingyue Chen Mingyue Chen Mingyue Chen Mary Tachedjian Glenn A. Marsh Jie Cui Jie Cui Lin-Fa Wang Lin-Fa Wang |
| author_sort | Mingyue Chen |
| collection | DOAJ |
| description | Hendra virus (HeV) and Cedar virus (CedV) are henipaviruses, which fall into the Paramyxoviridae family of single-stranded, negative-sense RNA viruses. HeV is classified as a Biosafety Level-4 (BSL-4) agent, as it is highly pathogenic and is often fatal to humans. To date, no HeV prevention or treatment methods for human are available. In contrast, previous experimental infection studies have suggested that CedV is non-pathogenic. Flying foxes (pteropid bats) in Australia are the natural reservoirs of both viruses, but the cellular responses of bats to these viral infections remain unclear. Here, we infected bat and human cells with these viruses. We then examined the total transcriptomic landscapes of the cells at 6 or 24 h post infection. Unexpectedly, despite the close phylogenetic relationship between HeV and CedV, there was a dramatic difference in cellular gene expression patterns in response to the two different infections. It is likely that minor differences in the phosphoprotein (P) gene coding strategy between the two viruses cause the observed incongruence in host transcriptomic divergence and viral lethality. This study greatly expands our understanding of the pathogenic mechanisms of henipaviruses. |
| first_indexed | 2024-12-12T18:25:12Z |
| format | Article |
| id | doaj.art-61fa85021cc34bc1a0de1d661d8641e1 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-12T18:25:12Z |
| publishDate | 2020-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-61fa85021cc34bc1a0de1d661d8641e12022-12-22T00:16:02ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-05-011110.3389/fmicb.2020.00986528301Distinct Cell Transcriptomic Landscapes Upon Henipavirus InfectionsMingyue Chen0Mingyue Chen1Mingyue Chen2Mary Tachedjian3Glenn A. Marsh4Jie Cui5Jie Cui6Lin-Fa Wang7Lin-Fa Wang8Key Laboratory of Fermentation Engineering, National 111 Center for Cellular Regulation and Molecular Pharmaceutics, Hubei University of Technology, Wuhan, ChinaCAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, ChinaCenter for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, ChinaCAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, ChinaAustralian Animal Health Laboratory, CSIRO Health and Biosecurity, Geelong, VIC, AustraliaCAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, ChinaCenter for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, ChinaAustralian Animal Health Laboratory, CSIRO Health and Biosecurity, Geelong, VIC, AustraliaProgramme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, SingaporeHendra virus (HeV) and Cedar virus (CedV) are henipaviruses, which fall into the Paramyxoviridae family of single-stranded, negative-sense RNA viruses. HeV is classified as a Biosafety Level-4 (BSL-4) agent, as it is highly pathogenic and is often fatal to humans. To date, no HeV prevention or treatment methods for human are available. In contrast, previous experimental infection studies have suggested that CedV is non-pathogenic. Flying foxes (pteropid bats) in Australia are the natural reservoirs of both viruses, but the cellular responses of bats to these viral infections remain unclear. Here, we infected bat and human cells with these viruses. We then examined the total transcriptomic landscapes of the cells at 6 or 24 h post infection. Unexpectedly, despite the close phylogenetic relationship between HeV and CedV, there was a dramatic difference in cellular gene expression patterns in response to the two different infections. It is likely that minor differences in the phosphoprotein (P) gene coding strategy between the two viruses cause the observed incongruence in host transcriptomic divergence and viral lethality. This study greatly expands our understanding of the pathogenic mechanisms of henipaviruses.https://www.frontiersin.org/article/10.3389/fmicb.2020.00986/fullbats (Chiroptera)Hendra virus (HeV)Cedar virustranscriptomaticscell infection |
| spellingShingle | Mingyue Chen Mingyue Chen Mingyue Chen Mary Tachedjian Glenn A. Marsh Jie Cui Jie Cui Lin-Fa Wang Lin-Fa Wang Distinct Cell Transcriptomic Landscapes Upon Henipavirus Infections Frontiers in Microbiology bats (Chiroptera) Hendra virus (HeV) Cedar virus transcriptomatics cell infection |
| title | Distinct Cell Transcriptomic Landscapes Upon Henipavirus Infections |
| title_full | Distinct Cell Transcriptomic Landscapes Upon Henipavirus Infections |
| title_fullStr | Distinct Cell Transcriptomic Landscapes Upon Henipavirus Infections |
| title_full_unstemmed | Distinct Cell Transcriptomic Landscapes Upon Henipavirus Infections |
| title_short | Distinct Cell Transcriptomic Landscapes Upon Henipavirus Infections |
| title_sort | distinct cell transcriptomic landscapes upon henipavirus infections |
| topic | bats (Chiroptera) Hendra virus (HeV) Cedar virus transcriptomatics cell infection |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2020.00986/full |
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