Determination of superoxide anion radicals in sodium hyposnlfite solution by chemiluminesceot methods(连二亚硫酸钠体系中超氧阴离子的化学发光法检测)

建立一种以luminol试剂为发光底物,检测连二亚硫酸钠水溶液体系产生的超氧阴离子自由基的化学发光法.研究表明,当luminol的浓度为0.05 mg/mL,连二亚硫酸钠浓度为0.012 mg/mL,体系pH=10.3时,可得最佳测定条件.分别测定了茶多酚、芦丁、槲皮素和抗坏血酸清除超氧阴离子自由基()的能力.结果表明此方法操作简便、测量快速,能用于天然活性物质清除超氧阴离子能力的测定和清除自由基机理的研究....

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Main Authors: SONGWei-hua(宋伟华), YANWei(阎魏), JIAZhi-shen(贾之慎)
Format: Article
Language:zho
Published: Zhejiang University Press 2007-09-01
Series:Zhejiang Daxue xuebao. Lixue ban
Subjects:
Online Access:https://doi.org/zjup/1008-9497.2007.34.5.538-540
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author SONGWei-hua(宋伟华)
YANWei(阎魏)
JIAZhi-shen(贾之慎)
author_facet SONGWei-hua(宋伟华)
YANWei(阎魏)
JIAZhi-shen(贾之慎)
author_sort SONGWei-hua(宋伟华)
collection DOAJ
description 建立一种以luminol试剂为发光底物,检测连二亚硫酸钠水溶液体系产生的超氧阴离子自由基的化学发光法.研究表明,当luminol的浓度为0.05 mg/mL,连二亚硫酸钠浓度为0.012 mg/mL,体系pH=10.3时,可得最佳测定条件.分别测定了茶多酚、芦丁、槲皮素和抗坏血酸清除超氧阴离子自由基()的能力.结果表明此方法操作简便、测量快速,能用于天然活性物质清除超氧阴离子能力的测定和清除自由基机理的研究.
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spelling doaj.art-61fb871195c14ae2ba97571fd9bf92c32024-03-29T01:58:25ZzhoZhejiang University PressZhejiang Daxue xuebao. Lixue ban1008-94972007-09-01345538540zjup/1008-9497.2007.34.5.538-540Determination of superoxide anion radicals in sodium hyposnlfite solution by chemiluminesceot methods(连二亚硫酸钠体系中超氧阴离子的化学发光法检测)SONGWei-hua(宋伟华)0YANWei(阎魏)1JIAZhi-shen(贾之慎)2Department of Chemistry, Zhejiang University, Hangzhou 310027, China(浙江大学化学系,浙江 杭州 310027)Department of Chemistry, Zhejiang University, Hangzhou 310027, China(浙江大学化学系,浙江 杭州 310027)Department of Chemistry, Zhejiang University, Hangzhou 310027, China(浙江大学化学系,浙江 杭州 310027)建立一种以luminol试剂为发光底物,检测连二亚硫酸钠水溶液体系产生的超氧阴离子自由基的化学发光法.研究表明,当luminol的浓度为0.05 mg/mL,连二亚硫酸钠浓度为0.012 mg/mL,体系pH=10.3时,可得最佳测定条件.分别测定了茶多酚、芦丁、槲皮素和抗坏血酸清除超氧阴离子自由基()的能力.结果表明此方法操作简便、测量快速,能用于天然活性物质清除超氧阴离子能力的测定和清除自由基机理的研究.https://doi.org/zjup/1008-9497.2007.34.5.538-540化学发光超氧阴离子自由基luminol连二亚硫酸钠
spellingShingle SONGWei-hua(宋伟华)
YANWei(阎魏)
JIAZhi-shen(贾之慎)
Determination of superoxide anion radicals in sodium hyposnlfite solution by chemiluminesceot methods(连二亚硫酸钠体系中超氧阴离子的化学发光法检测)
Zhejiang Daxue xuebao. Lixue ban
化学发光
超氧阴离子
自由基
luminol
连二亚硫酸钠
title Determination of superoxide anion radicals in sodium hyposnlfite solution by chemiluminesceot methods(连二亚硫酸钠体系中超氧阴离子的化学发光法检测)
title_full Determination of superoxide anion radicals in sodium hyposnlfite solution by chemiluminesceot methods(连二亚硫酸钠体系中超氧阴离子的化学发光法检测)
title_fullStr Determination of superoxide anion radicals in sodium hyposnlfite solution by chemiluminesceot methods(连二亚硫酸钠体系中超氧阴离子的化学发光法检测)
title_full_unstemmed Determination of superoxide anion radicals in sodium hyposnlfite solution by chemiluminesceot methods(连二亚硫酸钠体系中超氧阴离子的化学发光法检测)
title_short Determination of superoxide anion radicals in sodium hyposnlfite solution by chemiluminesceot methods(连二亚硫酸钠体系中超氧阴离子的化学发光法检测)
title_sort determination of superoxide anion radicals in sodium hyposnlfite solution by chemiluminesceot methods 连二亚硫酸钠体系中超氧阴离子的化学发光法检测
topic 化学发光
超氧阴离子
自由基
luminol
连二亚硫酸钠
url https://doi.org/zjup/1008-9497.2007.34.5.538-540
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