Metabonomic Insights into the Sperm Activation Mechanisms in Ricefield Eel (<i>Monopterus albus</i>)
In fish, sperm motility activation is one of the most essential procedures for fertilization. Previous studies have mainly focused on the external environmental effects and intracellular signals in sperm activation; however, little is known about the metabolic process of sperm motility activation in...
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MDPI AG
2020-10-01
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| Series: | Genes |
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| author | Huiying Zhang Yang Liu Lingling Zhou Shaohua Xu Cheng Ye Haifeng Tian Zhong Li Guangfu Hu |
| author_facet | Huiying Zhang Yang Liu Lingling Zhou Shaohua Xu Cheng Ye Haifeng Tian Zhong Li Guangfu Hu |
| author_sort | Huiying Zhang |
| collection | DOAJ |
| description | In fish, sperm motility activation is one of the most essential procedures for fertilization. Previous studies have mainly focused on the external environmental effects and intracellular signals in sperm activation; however, little is known about the metabolic process of sperm motility activation in fish. In the present study, using ricefield eel (<i>Monopterus albus</i>) sperm as a model, metabonomics was used to analyze the metabolic mechanism of the sperm motility activation in fish. Firstly, 529 metabolites were identified in the sperm of ricefield eel, which were clustered into the organic acids, amino acids, nucleotides, benzene, and carbohydrates, respectively. Among them, the most abundant metabolites in sperm were L-phenylalanine, DL-leucine, L-leucine, lysolecithin choline 18:0, L-tryptophan, adenine, hypoxanthine, 7-Methylguanine, shikimic acid, and L-tyrosine. Secondly, compared to pre-activated sperm, the level of S-sulfo-L-cysteine and L-asparagine were both increased in the post-activated sperm. Ninety-two metabolites were decreased in the post-activated sperm, including quinic acid, acetylsalicylic acid, 7,8-dihydro L-biopterin, citric acid, glycylphenylalanine, and dihydrotachysterol (DHT). Finally, basing on the pathway analysis, we found that the changed metabolites in sperm motility activation were mainly clustered into energy metabolism and anti-oxidative stress. Fish sperm motility activation would be accompanied by the release of a large amount of energy, which might damage the genetic material of sperm. Thus, the anti-oxidative stress function is a critical process to maintain the normal physiological function of sperm. |
| first_indexed | 2024-03-10T15:20:42Z |
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| last_indexed | 2024-03-10T15:20:42Z |
| publishDate | 2020-10-01 |
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| series | Genes |
| spelling | doaj.art-61fcf99204e24f47ae82b34ade898b472023-11-20T18:31:15ZengMDPI AGGenes2073-44252020-10-011111125910.3390/genes11111259Metabonomic Insights into the Sperm Activation Mechanisms in Ricefield Eel (<i>Monopterus albus</i>)Huiying Zhang0Yang Liu1Lingling Zhou2Shaohua Xu3Cheng Ye4Haifeng Tian5Zhong Li6Guangfu Hu7College of Fisheries, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Fisheries, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Fisheries, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Fisheries, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Fisheries, Huazhong Agricultural University, Wuhan 430070, ChinaKey Lab of Freshwater Biodiversity Conservation Ministry of Agriculture, Yangtze River Fisheries Research Institute, The Chinese Academy of Fisheries Sciences, Wuhan 430223, ChinaKey Lab of Freshwater Biodiversity Conservation Ministry of Agriculture, Yangtze River Fisheries Research Institute, The Chinese Academy of Fisheries Sciences, Wuhan 430223, ChinaCollege of Fisheries, Huazhong Agricultural University, Wuhan 430070, ChinaIn fish, sperm motility activation is one of the most essential procedures for fertilization. Previous studies have mainly focused on the external environmental effects and intracellular signals in sperm activation; however, little is known about the metabolic process of sperm motility activation in fish. In the present study, using ricefield eel (<i>Monopterus albus</i>) sperm as a model, metabonomics was used to analyze the metabolic mechanism of the sperm motility activation in fish. Firstly, 529 metabolites were identified in the sperm of ricefield eel, which were clustered into the organic acids, amino acids, nucleotides, benzene, and carbohydrates, respectively. Among them, the most abundant metabolites in sperm were L-phenylalanine, DL-leucine, L-leucine, lysolecithin choline 18:0, L-tryptophan, adenine, hypoxanthine, 7-Methylguanine, shikimic acid, and L-tyrosine. Secondly, compared to pre-activated sperm, the level of S-sulfo-L-cysteine and L-asparagine were both increased in the post-activated sperm. Ninety-two metabolites were decreased in the post-activated sperm, including quinic acid, acetylsalicylic acid, 7,8-dihydro L-biopterin, citric acid, glycylphenylalanine, and dihydrotachysterol (DHT). Finally, basing on the pathway analysis, we found that the changed metabolites in sperm motility activation were mainly clustered into energy metabolism and anti-oxidative stress. Fish sperm motility activation would be accompanied by the release of a large amount of energy, which might damage the genetic material of sperm. Thus, the anti-oxidative stress function is a critical process to maintain the normal physiological function of sperm.https://www.mdpi.com/2073-4425/11/11/1259sperm activationmetabonomicsenergy metabolismanti-oxidant stressricefield eel |
| spellingShingle | Huiying Zhang Yang Liu Lingling Zhou Shaohua Xu Cheng Ye Haifeng Tian Zhong Li Guangfu Hu Metabonomic Insights into the Sperm Activation Mechanisms in Ricefield Eel (<i>Monopterus albus</i>) Genes sperm activation metabonomics energy metabolism anti-oxidant stress ricefield eel |
| title | Metabonomic Insights into the Sperm Activation Mechanisms in Ricefield Eel (<i>Monopterus albus</i>) |
| title_full | Metabonomic Insights into the Sperm Activation Mechanisms in Ricefield Eel (<i>Monopterus albus</i>) |
| title_fullStr | Metabonomic Insights into the Sperm Activation Mechanisms in Ricefield Eel (<i>Monopterus albus</i>) |
| title_full_unstemmed | Metabonomic Insights into the Sperm Activation Mechanisms in Ricefield Eel (<i>Monopterus albus</i>) |
| title_short | Metabonomic Insights into the Sperm Activation Mechanisms in Ricefield Eel (<i>Monopterus albus</i>) |
| title_sort | metabonomic insights into the sperm activation mechanisms in ricefield eel i monopterus albus i |
| topic | sperm activation metabonomics energy metabolism anti-oxidant stress ricefield eel |
| url | https://www.mdpi.com/2073-4425/11/11/1259 |
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