The Effect of Foliar Selenium (Se) Treatment on Growth, Photosynthesis, and Oxidative-Nitrosative Signalling of <i>Stevia rebaudiana</i> Leaves
Selenium (Se) enrichment of <i>Stevia rebaudiana</i> Bertoni can serve a dual purpose, on the one hand to increase plant biomass and stress tolerance and on the other hand to produce Se fortified plant-based food. Foliar Se spraying (0, 6, 8, 10 mg/L selenate, 14 days) of <i>Stevia...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-01-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/1/72 |
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| author | Péter Borbély Árpád Molnár Emil Valyon Attila Ördög Klára Horváth-Boros Dezső Csupor Attila Fehér Zsuzsanna Kolbert |
| author_facet | Péter Borbély Árpád Molnár Emil Valyon Attila Ördög Klára Horváth-Boros Dezső Csupor Attila Fehér Zsuzsanna Kolbert |
| author_sort | Péter Borbély |
| collection | DOAJ |
| description | Selenium (Se) enrichment of <i>Stevia rebaudiana</i> Bertoni can serve a dual purpose, on the one hand to increase plant biomass and stress tolerance and on the other hand to produce Se fortified plant-based food. Foliar Se spraying (0, 6, 8, 10 mg/L selenate, 14 days) of <i>Stevia</i> plantlets resulted in slightly decreased stevioside and rebaudioside A concentrations, and it also caused significant increment in stem elongation, leaf number, and Se content, suggesting that foliar Se supplementation can be used as a biofortifying approach. Furthermore, Se slightly limited photosynthetic CO<sub>2</sub> assimilation (A<sub>N</sub>, g<sub>sw</sub>, C<sub>i</sub>/C<sub>a</sub>), but exerted no significant effect on chlorophyll, carotenoid contents and on parameters associated with photosystem II (PSII) activity (F<sub>V</sub>/F<sub>M</sub>, F<sub>0</sub>, Y(NO)), indicating that Se causes no photodamage in PSII. Further results indicate that Se is able to activate PSI-cyclic electron flow independent protection mechanisms of the photosynthetic apparatus of <i>Stevia</i> plants. The applied Se activated superoxide dismutase (SOD) isoenzymes (MnSOD1, FeSOD1, FeSOD2, Cu/ZnSOD1, Cu/ZnSOD2) and down-regulated NADPH oxidase suggesting the Se-induced limitation of superoxide anion levels and consequent oxidative signalling in <i>Stevia</i> leaves. Additionally, the decrease in S-nitrosoglutathione reductase protein abundance and the intensification of protein tyrosine nitration indicate Se-triggered nitrosative signalling. Collectively, these results suggest that Se supplementation alters <i>Stevia</i> shoot morphology without significantly affecting biomass yield and photosynthesis, but increasing Se content and performing antioxidant effects, which indicates that foliar application of Se may be a promising method in <i>Stevia</i> cultivation. |
| first_indexed | 2024-03-09T05:41:08Z |
| format | Article |
| id | doaj.art-61fd0653bfc64fa5a8dc80116c081126 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T05:41:08Z |
| publishDate | 2021-01-01 |
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| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-61fd0653bfc64fa5a8dc80116c0811262023-12-03T12:25:05ZengMDPI AGAntioxidants2076-39212021-01-011017210.3390/antiox10010072The Effect of Foliar Selenium (Se) Treatment on Growth, Photosynthesis, and Oxidative-Nitrosative Signalling of <i>Stevia rebaudiana</i> LeavesPéter Borbély0Árpád Molnár1Emil Valyon2Attila Ördög3Klára Horváth-Boros4Dezső Csupor5Attila Fehér6Zsuzsanna Kolbert7Department of Plant Biology, University of Szeged, Közép Fasor 52, H-6726 Szeged, HungaryDepartment of Plant Biology, University of Szeged, Közép Fasor 52, H-6726 Szeged, HungaryDepartment of Plant Biology, University of Szeged, Közép Fasor 52, H-6726 Szeged, HungaryDepartment of Plant Biology, University of Szeged, Közép Fasor 52, H-6726 Szeged, HungaryDepartment of Pharmacognosy, Faculty of Pharmacy, University of Szeged, Eötvös u. 6, H-6720 Szeged, HungaryDepartment of Pharmacognosy, Faculty of Pharmacy, University of Szeged, Eötvös u. 6, H-6720 Szeged, HungaryDepartment of Plant Biology, University of Szeged, Közép Fasor 52, H-6726 Szeged, HungaryDepartment of Plant Biology, University of Szeged, Közép Fasor 52, H-6726 Szeged, HungarySelenium (Se) enrichment of <i>Stevia rebaudiana</i> Bertoni can serve a dual purpose, on the one hand to increase plant biomass and stress tolerance and on the other hand to produce Se fortified plant-based food. Foliar Se spraying (0, 6, 8, 10 mg/L selenate, 14 days) of <i>Stevia</i> plantlets resulted in slightly decreased stevioside and rebaudioside A concentrations, and it also caused significant increment in stem elongation, leaf number, and Se content, suggesting that foliar Se supplementation can be used as a biofortifying approach. Furthermore, Se slightly limited photosynthetic CO<sub>2</sub> assimilation (A<sub>N</sub>, g<sub>sw</sub>, C<sub>i</sub>/C<sub>a</sub>), but exerted no significant effect on chlorophyll, carotenoid contents and on parameters associated with photosystem II (PSII) activity (F<sub>V</sub>/F<sub>M</sub>, F<sub>0</sub>, Y(NO)), indicating that Se causes no photodamage in PSII. Further results indicate that Se is able to activate PSI-cyclic electron flow independent protection mechanisms of the photosynthetic apparatus of <i>Stevia</i> plants. The applied Se activated superoxide dismutase (SOD) isoenzymes (MnSOD1, FeSOD1, FeSOD2, Cu/ZnSOD1, Cu/ZnSOD2) and down-regulated NADPH oxidase suggesting the Se-induced limitation of superoxide anion levels and consequent oxidative signalling in <i>Stevia</i> leaves. Additionally, the decrease in S-nitrosoglutathione reductase protein abundance and the intensification of protein tyrosine nitration indicate Se-triggered nitrosative signalling. Collectively, these results suggest that Se supplementation alters <i>Stevia</i> shoot morphology without significantly affecting biomass yield and photosynthesis, but increasing Se content and performing antioxidant effects, which indicates that foliar application of Se may be a promising method in <i>Stevia</i> cultivation.https://www.mdpi.com/2076-3921/10/1/72nitrosative signallingoxidative signallingphotosynthesisselenium<i>Stevia rebaudiana</i> |
| spellingShingle | Péter Borbély Árpád Molnár Emil Valyon Attila Ördög Klára Horváth-Boros Dezső Csupor Attila Fehér Zsuzsanna Kolbert The Effect of Foliar Selenium (Se) Treatment on Growth, Photosynthesis, and Oxidative-Nitrosative Signalling of <i>Stevia rebaudiana</i> Leaves Antioxidants nitrosative signalling oxidative signalling photosynthesis selenium <i>Stevia rebaudiana</i> |
| title | The Effect of Foliar Selenium (Se) Treatment on Growth, Photosynthesis, and Oxidative-Nitrosative Signalling of <i>Stevia rebaudiana</i> Leaves |
| title_full | The Effect of Foliar Selenium (Se) Treatment on Growth, Photosynthesis, and Oxidative-Nitrosative Signalling of <i>Stevia rebaudiana</i> Leaves |
| title_fullStr | The Effect of Foliar Selenium (Se) Treatment on Growth, Photosynthesis, and Oxidative-Nitrosative Signalling of <i>Stevia rebaudiana</i> Leaves |
| title_full_unstemmed | The Effect of Foliar Selenium (Se) Treatment on Growth, Photosynthesis, and Oxidative-Nitrosative Signalling of <i>Stevia rebaudiana</i> Leaves |
| title_short | The Effect of Foliar Selenium (Se) Treatment on Growth, Photosynthesis, and Oxidative-Nitrosative Signalling of <i>Stevia rebaudiana</i> Leaves |
| title_sort | effect of foliar selenium se treatment on growth photosynthesis and oxidative nitrosative signalling of i stevia rebaudiana i leaves |
| topic | nitrosative signalling oxidative signalling photosynthesis selenium <i>Stevia rebaudiana</i> |
| url | https://www.mdpi.com/2076-3921/10/1/72 |
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