NKp80 Defines a Critical Step during Human Natural Killer Cell Development
Human natural killer (NK) cells develop in secondary lymphoid tissues (SLTs) through distinct stages. We identified two SLT lineage (Lin)−CD34−CD117+/−CD94+CD16− “stage 4” subsets according to expression of the C-type lectin-like surface-activating receptor, NKp80: NKp80− (stage “4a”) and NKp80+ (st...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2016-07-01
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| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124716307367 |
| _version_ | 1817997407967772672 |
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| author | Aharon G. Freud Karen A. Keller Steven D. Scoville Bethany L. Mundy-Bosse Stephanie Cheng Youssef Youssef Tiffany Hughes Xiaoli Zhang Xiaokui Mo Pierluigi Porcu Robert A. Baiocchi Jianhua Yu William E. Carson III Michael A. Caligiuri |
| author_facet | Aharon G. Freud Karen A. Keller Steven D. Scoville Bethany L. Mundy-Bosse Stephanie Cheng Youssef Youssef Tiffany Hughes Xiaoli Zhang Xiaokui Mo Pierluigi Porcu Robert A. Baiocchi Jianhua Yu William E. Carson III Michael A. Caligiuri |
| author_sort | Aharon G. Freud |
| collection | DOAJ |
| description | Human natural killer (NK) cells develop in secondary lymphoid tissues (SLTs) through distinct stages. We identified two SLT lineage (Lin)−CD34−CD117+/−CD94+CD16− “stage 4” subsets according to expression of the C-type lectin-like surface-activating receptor, NKp80: NKp80− (stage “4a”) and NKp80+ (stage “4b”). Whereas stage 4b cells expressed more of the transcription factors T-BET and EOMES, produced interferon-gamma, and were cytotoxic, stage 4a cells expressed more of the transcription factors RORγt and AHR and produced interleukin-22, similar to SLT Lin−CD34−CD117+CD94−CD16− “stage 3” cells, whose phenotype overlaps with that of group 3 innate lymphoid cells (ILC3s). Co-culture with dendritic cells or transplantation into immunodeficient mice produced mature NK cells from stage 3 and stage 4a populations. These data identify NKp80 as a marker of NK cell maturity in SLTs and support a model of human NK cell development through a stage 4a intermediate with ILC3-associated features. |
| first_indexed | 2024-04-14T02:37:45Z |
| format | Article |
| id | doaj.art-61fd41d421fe400498c6b805a85b7b19 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-04-14T02:37:45Z |
| publishDate | 2016-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-61fd41d421fe400498c6b805a85b7b192022-12-22T02:17:19ZengElsevierCell Reports2211-12472016-07-0116237939110.1016/j.celrep.2016.05.095NKp80 Defines a Critical Step during Human Natural Killer Cell DevelopmentAharon G. Freud0Karen A. Keller1Steven D. Scoville2Bethany L. Mundy-Bosse3Stephanie Cheng4Youssef Youssef5Tiffany Hughes6Xiaoli Zhang7Xiaokui Mo8Pierluigi Porcu9Robert A. Baiocchi10Jianhua Yu11William E. Carson III12Michael A. Caligiuri13Department of Pathology, The Ohio State University, Columbus, OH 43210, USADepartment of Pathology, The Ohio State University, Columbus, OH 43210, USAMedical Scientist Training Program, The Ohio State University, Columbus, OH 43210, USADivision of Hematology, Department of Internal Medicine, The Ohio State University, Columbus, OH 43210, USADepartment of Pathology, The Ohio State University, Columbus, OH 43210, USADepartment of Pathology, The Ohio State University, Columbus, OH 43210, USADivision of Hematology, Department of Internal Medicine, The Ohio State University, Columbus, OH 43210, USACenter for Biostatistics, The Ohio State University, Columbus, OH 43210, USACenter for Biostatistics, The Ohio State University, Columbus, OH 43210, USADivision of Hematology, Department of Internal Medicine, The Ohio State University, Columbus, OH 43210, USADivision of Hematology, Department of Internal Medicine, The Ohio State University, Columbus, OH 43210, USADivision of Hematology, Department of Internal Medicine, The Ohio State University, Columbus, OH 43210, USADivision of Surgical Oncology, Department of Surgery, The Ohio State University, Columbus, OH 43210, USADivision of Hematology, Department of Internal Medicine, The Ohio State University, Columbus, OH 43210, USAHuman natural killer (NK) cells develop in secondary lymphoid tissues (SLTs) through distinct stages. We identified two SLT lineage (Lin)−CD34−CD117+/−CD94+CD16− “stage 4” subsets according to expression of the C-type lectin-like surface-activating receptor, NKp80: NKp80− (stage “4a”) and NKp80+ (stage “4b”). Whereas stage 4b cells expressed more of the transcription factors T-BET and EOMES, produced interferon-gamma, and were cytotoxic, stage 4a cells expressed more of the transcription factors RORγt and AHR and produced interleukin-22, similar to SLT Lin−CD34−CD117+CD94−CD16− “stage 3” cells, whose phenotype overlaps with that of group 3 innate lymphoid cells (ILC3s). Co-culture with dendritic cells or transplantation into immunodeficient mice produced mature NK cells from stage 3 and stage 4a populations. These data identify NKp80 as a marker of NK cell maturity in SLTs and support a model of human NK cell development through a stage 4a intermediate with ILC3-associated features.http://www.sciencedirect.com/science/article/pii/S2211124716307367 |
| spellingShingle | Aharon G. Freud Karen A. Keller Steven D. Scoville Bethany L. Mundy-Bosse Stephanie Cheng Youssef Youssef Tiffany Hughes Xiaoli Zhang Xiaokui Mo Pierluigi Porcu Robert A. Baiocchi Jianhua Yu William E. Carson III Michael A. Caligiuri NKp80 Defines a Critical Step during Human Natural Killer Cell Development Cell Reports |
| title | NKp80 Defines a Critical Step during Human Natural Killer Cell Development |
| title_full | NKp80 Defines a Critical Step during Human Natural Killer Cell Development |
| title_fullStr | NKp80 Defines a Critical Step during Human Natural Killer Cell Development |
| title_full_unstemmed | NKp80 Defines a Critical Step during Human Natural Killer Cell Development |
| title_short | NKp80 Defines a Critical Step during Human Natural Killer Cell Development |
| title_sort | nkp80 defines a critical step during human natural killer cell development |
| url | http://www.sciencedirect.com/science/article/pii/S2211124716307367 |
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