Effect of High BMI on Human Bone Marrow–Derived Mesenchymal Stromal Cells
Bone marrow–derived mesenchymal stromal cells (BMSCs) are attractive candidates in tissue engineering and regenerative medicine. Growing evidence has suggested that a high body mass index (BMI) can affect the properties of BMSCs, resulting in a reduced quality of the cells. However, the results are...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2024-01-01
|
| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.1177/09636897241226546 |
| _version_ | 1797337612458917888 |
|---|---|
| author | Qiang Zong Katrin Bundkirchen Claudia Neunaber Sandra Noack |
| author_facet | Qiang Zong Katrin Bundkirchen Claudia Neunaber Sandra Noack |
| author_sort | Qiang Zong |
| collection | DOAJ |
| description | Bone marrow–derived mesenchymal stromal cells (BMSCs) are attractive candidates in tissue engineering and regenerative medicine. Growing evidence has suggested that a high body mass index (BMI) can affect the properties of BMSCs, resulting in a reduced quality of the cells. However, the results are not consistent. Therefore, this study aimed to investigate the influences of high BMI on human BMSCs (hBMSCs). To avoid gender bias, BMSCs from females and males were studied independently. Finally, hBMSCs from 89 females and 152 males were separately divided into the normal BMI group (18.5 kg/m 2 ≤ BMI < 25 kg/m 2 ) and the high BMI group (BMI > 25 kg/m 2 ). The cells were analyzed for the colony-forming potential; proliferation capacity; in vitro adipogenic, osteogenic, and chondrogenic differentiation potentials; and the expression of 32 common surface antigens. The results showed that high BMI did not change the number of colonies at passage 1 in females and males. In contrast, significantly reduced colony numbers at passage 4 (P4) were found in both female and male donors with high BMI. The doubling time of hBMSCs was comparable between the normal and the high BMI groups of females and males. Furthermore, the results of trilineage differentiation did not differ between the different BMI groups of males. In females, the high and the normal BMI groups also showed similar adipogenic and chondrogenic differentiation, while osteogenic differentiation was significantly enhanced in the high-BMI group. Regarding the expression of surface antigens, the expressions of CD200 and SSEA4 on hBMSCs were reduced in the high-BMI group of females and males, respectively. In conclusion, high BMI suppressed the clonogenicity of female and male hBMSCs at P4, improved the in vitro osteogenesis of female hBMSCs, and decreased the expressions of CD200 on hBMSCs in females and SSEA4 in males. |
| first_indexed | 2024-03-08T09:12:13Z |
| format | Article |
| id | doaj.art-62043e1f561a44edacf2a56e0332dd9b |
| institution | Directory Open Access Journal |
| issn | 1555-3892 |
| language | English |
| last_indexed | 2024-03-08T09:12:13Z |
| publishDate | 2024-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-62043e1f561a44edacf2a56e0332dd9b2024-01-31T19:03:33ZengSAGE PublishingCell Transplantation1555-38922024-01-013310.1177/09636897241226546Effect of High BMI on Human Bone Marrow–Derived Mesenchymal Stromal CellsQiang Zong0Katrin Bundkirchen1Claudia Neunaber2Sandra Noack3Department of Trauma Surgery, Hannover Medical School, Hannover, GermanyDepartment of Trauma Surgery, Hannover Medical School, Hannover, GermanyDepartment of Trauma Surgery, Hannover Medical School, Hannover, GermanyDepartment of Trauma Surgery, Hannover Medical School, Hannover, GermanyBone marrow–derived mesenchymal stromal cells (BMSCs) are attractive candidates in tissue engineering and regenerative medicine. Growing evidence has suggested that a high body mass index (BMI) can affect the properties of BMSCs, resulting in a reduced quality of the cells. However, the results are not consistent. Therefore, this study aimed to investigate the influences of high BMI on human BMSCs (hBMSCs). To avoid gender bias, BMSCs from females and males were studied independently. Finally, hBMSCs from 89 females and 152 males were separately divided into the normal BMI group (18.5 kg/m 2 ≤ BMI < 25 kg/m 2 ) and the high BMI group (BMI > 25 kg/m 2 ). The cells were analyzed for the colony-forming potential; proliferation capacity; in vitro adipogenic, osteogenic, and chondrogenic differentiation potentials; and the expression of 32 common surface antigens. The results showed that high BMI did not change the number of colonies at passage 1 in females and males. In contrast, significantly reduced colony numbers at passage 4 (P4) were found in both female and male donors with high BMI. The doubling time of hBMSCs was comparable between the normal and the high BMI groups of females and males. Furthermore, the results of trilineage differentiation did not differ between the different BMI groups of males. In females, the high and the normal BMI groups also showed similar adipogenic and chondrogenic differentiation, while osteogenic differentiation was significantly enhanced in the high-BMI group. Regarding the expression of surface antigens, the expressions of CD200 and SSEA4 on hBMSCs were reduced in the high-BMI group of females and males, respectively. In conclusion, high BMI suppressed the clonogenicity of female and male hBMSCs at P4, improved the in vitro osteogenesis of female hBMSCs, and decreased the expressions of CD200 on hBMSCs in females and SSEA4 in males.https://doi.org/10.1177/09636897241226546 |
| spellingShingle | Qiang Zong Katrin Bundkirchen Claudia Neunaber Sandra Noack Effect of High BMI on Human Bone Marrow–Derived Mesenchymal Stromal Cells Cell Transplantation |
| title | Effect of High BMI on Human Bone Marrow–Derived Mesenchymal Stromal Cells |
| title_full | Effect of High BMI on Human Bone Marrow–Derived Mesenchymal Stromal Cells |
| title_fullStr | Effect of High BMI on Human Bone Marrow–Derived Mesenchymal Stromal Cells |
| title_full_unstemmed | Effect of High BMI on Human Bone Marrow–Derived Mesenchymal Stromal Cells |
| title_short | Effect of High BMI on Human Bone Marrow–Derived Mesenchymal Stromal Cells |
| title_sort | effect of high bmi on human bone marrow derived mesenchymal stromal cells |
| url | https://doi.org/10.1177/09636897241226546 |
| work_keys_str_mv | AT qiangzong effectofhighbmionhumanbonemarrowderivedmesenchymalstromalcells AT katrinbundkirchen effectofhighbmionhumanbonemarrowderivedmesenchymalstromalcells AT claudianeunaber effectofhighbmionhumanbonemarrowderivedmesenchymalstromalcells AT sandranoack effectofhighbmionhumanbonemarrowderivedmesenchymalstromalcells |