| Summary: | An experiment was conducted to estimate the lethal dose of the chemical mutagen EMS and physical mutagen gamma ray in
barnyard millet variety Co(Kv) 2. Genetically pure seeds were treated with different doses of EMS viz. 30mM, 40mM,
50mM, 60mM, 70mM, 80mM, 90mM and 100mM and gamma rays viz., 500 Gy, 600 Gy, 700 Gy, 800 Gy, 900Gy and
1000 Gy. The LD50 values were observed based on growth reduction of seedlings after EMS and gamma ray treatment. The
LD50 dose for EMS and gamma ray under in vitro and in vivo condition were fixed at 70mM and 800 Gy respectively, based
on probit analysis. As the doses of applied EMS and gamma ray increased, there was a decrease in germination, survival rate
of seedlings, root length, shoot length, seedling height, vigour index under in vitro conditions and emergence and survival
under field (in vivo) conditions in M1 generation as compared to the control.
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