A Fast and Reliable UHPLC–MS/MS-Based Method for Screening Selected Pharmacologically Significant Natural Plant Indole Alkaloids
Many substances of secondary plant metabolism have often attracted the attention of scientists and the public because they have certain beneficial effects on human health, although the reason for their biosynthesis in the plant remains unclear. This is also the case for alkaloids. More than 200 year...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-07-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/14/3274 |
| _version_ | 1797562091946639360 |
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| author | Danuše Tarkowská |
| author_facet | Danuše Tarkowská |
| author_sort | Danuše Tarkowská |
| collection | DOAJ |
| description | Many substances of secondary plant metabolism have often attracted the attention of scientists and the public because they have certain beneficial effects on human health, although the reason for their biosynthesis in the plant remains unclear. This is also the case for alkaloids. More than 200 years have passed since the discovery of the first alkaloid (morphine), and several thousand substances of this character have been isolated since then. Most often, alkaloid-rich plants are part of folk medicine with centuries-old traditions. What is particularly important to monitor for these herbal products is the spectrum and concentrations of the present active substances, which decide whether the product has a beneficial or toxic effect on human health. In this work, we present a fast, reliable, and robust method for the extraction, preconcentration, and determination of four selected alkaloids with an indole skeleton, i.e., harmine, harmaline, yohimbine, and ajmalicine, by ultra-high performance liquid chromatography coupled with tandem mass spectrometry. The applicability of the method was demonstrated for tobacco and <i>Tribulus terrestris</i> plant tissue, the seeds of <i>Peganum harmala,</i> and extract from the bark of the African tree <i>Pausinystalia johimbe</i>. |
| first_indexed | 2024-03-10T18:23:41Z |
| format | Article |
| id | doaj.art-6207e399ec8b492684f6bba119bbf0c8 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T18:23:41Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-6207e399ec8b492684f6bba119bbf0c82023-11-20T07:10:16ZengMDPI AGMolecules1420-30492020-07-012514327410.3390/molecules25143274A Fast and Reliable UHPLC–MS/MS-Based Method for Screening Selected Pharmacologically Significant Natural Plant Indole AlkaloidsDanuše Tarkowská0Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany, Czech Academy of Sciences and Faculty of Science, Palacký University, CZ-78371 Olomouc, Czech RepublicMany substances of secondary plant metabolism have often attracted the attention of scientists and the public because they have certain beneficial effects on human health, although the reason for their biosynthesis in the plant remains unclear. This is also the case for alkaloids. More than 200 years have passed since the discovery of the first alkaloid (morphine), and several thousand substances of this character have been isolated since then. Most often, alkaloid-rich plants are part of folk medicine with centuries-old traditions. What is particularly important to monitor for these herbal products is the spectrum and concentrations of the present active substances, which decide whether the product has a beneficial or toxic effect on human health. In this work, we present a fast, reliable, and robust method for the extraction, preconcentration, and determination of four selected alkaloids with an indole skeleton, i.e., harmine, harmaline, yohimbine, and ajmalicine, by ultra-high performance liquid chromatography coupled with tandem mass spectrometry. The applicability of the method was demonstrated for tobacco and <i>Tribulus terrestris</i> plant tissue, the seeds of <i>Peganum harmala,</i> and extract from the bark of the African tree <i>Pausinystalia johimbe</i>.https://www.mdpi.com/1420-3049/25/14/3274monoterpene indole alkaloidsmonoamine oxidase inhibitorsharmalineharmineyohimbineajmalicine |
| spellingShingle | Danuše Tarkowská A Fast and Reliable UHPLC–MS/MS-Based Method for Screening Selected Pharmacologically Significant Natural Plant Indole Alkaloids Molecules monoterpene indole alkaloids monoamine oxidase inhibitors harmaline harmine yohimbine ajmalicine |
| title | A Fast and Reliable UHPLC–MS/MS-Based Method for Screening Selected Pharmacologically Significant Natural Plant Indole Alkaloids |
| title_full | A Fast and Reliable UHPLC–MS/MS-Based Method for Screening Selected Pharmacologically Significant Natural Plant Indole Alkaloids |
| title_fullStr | A Fast and Reliable UHPLC–MS/MS-Based Method for Screening Selected Pharmacologically Significant Natural Plant Indole Alkaloids |
| title_full_unstemmed | A Fast and Reliable UHPLC–MS/MS-Based Method for Screening Selected Pharmacologically Significant Natural Plant Indole Alkaloids |
| title_short | A Fast and Reliable UHPLC–MS/MS-Based Method for Screening Selected Pharmacologically Significant Natural Plant Indole Alkaloids |
| title_sort | fast and reliable uhplc ms ms based method for screening selected pharmacologically significant natural plant indole alkaloids |
| topic | monoterpene indole alkaloids monoamine oxidase inhibitors harmaline harmine yohimbine ajmalicine |
| url | https://www.mdpi.com/1420-3049/25/14/3274 |
| work_keys_str_mv | AT danusetarkowska afastandreliableuhplcmsmsbasedmethodforscreeningselectedpharmacologicallysignificantnaturalplantindolealkaloids AT danusetarkowska fastandreliableuhplcmsmsbasedmethodforscreeningselectedpharmacologicallysignificantnaturalplantindolealkaloids |