Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by Elisa
This study was done to assess an enzyme-linked immunosorbent assay (ELISA) to detect chicken serological response against Salmonella enterica serotypes Gallinarum and Pullorum. The assays have used soluble bacterial proteins of Salmonella Gallinarum strain 9 (AgSG) as detecting antigen and peroxidas...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Fundação APINCO de Ciência e Tecnologia Avícolas
2004-06-01
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| Series: | Brazilian Journal of Poultry Science |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000200007 |
| Summary: | This study was done to assess an enzyme-linked immunosorbent assay (ELISA) to detect chicken serological response against Salmonella enterica serotypes Gallinarum and Pullorum. The assays have used soluble bacterial proteins of Salmonella Gallinarum strain 9 (AgSG) as detecting antigen and peroxidase and alkaline phosphatase conjugates. According to the results, the antigen, sera and conjugate concentrations were optimized. In addition, the assay using alkaline phosphatase and peroxidase conjugates was helpful to distinguish positive serological reaction to serotypes Gallinarum and Pullorum from Enteritidis. |
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| ISSN: | 1516-635X 1806-9061 |