Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by Elisa
This study was done to assess an enzyme-linked immunosorbent assay (ELISA) to detect chicken serological response against Salmonella enterica serotypes Gallinarum and Pullorum. The assays have used soluble bacterial proteins of Salmonella Gallinarum strain 9 (AgSG) as detecting antigen and peroxidas...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Fundação APINCO de Ciência e Tecnologia Avícolas
2004-06-01
|
| Series: | Brazilian Journal of Poultry Science |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000200007 |
| _version_ | 1819195475636518912 |
|---|---|
| author | GH de<A NAME="nt01a"></A> Oliveira  Berchieri Júnior HJ Montassier AC Fernandes |
| author_facet | GH de<A NAME="nt01a"></A> Oliveira  Berchieri Júnior HJ Montassier AC Fernandes |
| author_sort | GH de<A NAME="nt01a"></A> Oliveira |
| collection | DOAJ |
| description | This study was done to assess an enzyme-linked immunosorbent assay (ELISA) to detect chicken serological response against Salmonella enterica serotypes Gallinarum and Pullorum. The assays have used soluble bacterial proteins of Salmonella Gallinarum strain 9 (AgSG) as detecting antigen and peroxidase and alkaline phosphatase conjugates. According to the results, the antigen, sera and conjugate concentrations were optimized. In addition, the assay using alkaline phosphatase and peroxidase conjugates was helpful to distinguish positive serological reaction to serotypes Gallinarum and Pullorum from Enteritidis. |
| first_indexed | 2024-12-23T02:13:21Z |
| format | Article |
| id | doaj.art-620cd14d2f634cc8a04bab6e9d61e56c |
| institution | Directory Open Access Journal |
| issn | 1516-635X 1806-9061 |
| language | English |
| last_indexed | 2024-12-23T02:13:21Z |
| publishDate | 2004-06-01 |
| publisher | Fundação APINCO de Ciência e Tecnologia Avícolas |
| record_format | Article |
| series | Brazilian Journal of Poultry Science |
| spelling | doaj.art-620cd14d2f634cc8a04bab6e9d61e56c2022-12-21T18:03:44ZengFundação APINCO de Ciência e Tecnologia AvícolasBrazilian Journal of Poultry Science1516-635X1806-90612004-06-016211111510.1590/S1516-635X2004000200007Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by ElisaGH de<A NAME="nt01a"></A> Oliveira Berchieri JúniorHJ MontassierAC FernandesThis study was done to assess an enzyme-linked immunosorbent assay (ELISA) to detect chicken serological response against Salmonella enterica serotypes Gallinarum and Pullorum. The assays have used soluble bacterial proteins of Salmonella Gallinarum strain 9 (AgSG) as detecting antigen and peroxidase and alkaline phosphatase conjugates. According to the results, the antigen, sera and conjugate concentrations were optimized. In addition, the assay using alkaline phosphatase and peroxidase conjugates was helpful to distinguish positive serological reaction to serotypes Gallinarum and Pullorum from Enteritidis.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000200007ChickensconjugateELISASalmonella spserology |
| spellingShingle | GH de<A NAME="nt01a"></A> Oliveira  Berchieri Júnior HJ Montassier AC Fernandes Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by Elisa Brazilian Journal of Poultry Science Chickens conjugate ELISA Salmonella sp serology |
| title | Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by Elisa |
| title_full | Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by Elisa |
| title_fullStr | Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by Elisa |
| title_full_unstemmed | Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by Elisa |
| title_short | Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by Elisa |
| title_sort | assessment of serological response of chickens to salmonella gallinarum and salmonella pullorum by elisa |
| topic | Chickens conjugate ELISA Salmonella sp serology |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000200007 |
| work_keys_str_mv | AT ghdeltanament01agtltagtoliveira assessmentofserologicalresponseofchickenstosalmonellagallinarumandsalmonellapullorumbyelisa AT aberchierijunior assessmentofserologicalresponseofchickenstosalmonellagallinarumandsalmonellapullorumbyelisa AT hjmontassier assessmentofserologicalresponseofchickenstosalmonellagallinarumandsalmonellapullorumbyelisa AT acfernandes assessmentofserologicalresponseofchickenstosalmonellagallinarumandsalmonellapullorumbyelisa |