Genome-Wide Identification and Expression Analysis of <i>NPF</i> Genes in Cucumber (<i>Cucumis sativus</i> L.)
The NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER family (NPF) proteins perform an essential role in regulating plant nitrate absorption and distribution and in improving plant nitrogen use efficiency. In this study, cucumber (<i>Cucumis sativus</i> L.) <i>NPF</i> genes were comp...
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MDPI AG
2023-03-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/6/1252 |
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| author | Mengying Zhang Wenyan Zhang Zijian Zheng Zhiping Zhang Bing Hua Jiexia Liu Minmin Miao |
| author_facet | Mengying Zhang Wenyan Zhang Zijian Zheng Zhiping Zhang Bing Hua Jiexia Liu Minmin Miao |
| author_sort | Mengying Zhang |
| collection | DOAJ |
| description | The NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER family (NPF) proteins perform an essential role in regulating plant nitrate absorption and distribution and in improving plant nitrogen use efficiency. In this study, cucumber (<i>Cucumis sativus</i> L.) <i>NPF</i> genes were comprehensively analyzed at the whole genome level, and 54 <i>NPF</i> genes were found to be unevenly distributed on seven chromosomes in the cucumber genome. The phylogenetic analysis showed that these genes could be divided into eight subfamilies. We renamed all <i>CsNPF</i> genes according to the international nomenclature, based on their homology with <i>AtNPF</i> genes. By surveying the expression profiles of <i>CsNPF</i> genes in various tissues, we found that <i>CsNPF6.4</i> was specifically expressed in roots, indicating that <i>CsNPF6.4</i> may play a role in N absorption; <i>CsNPF6.3</i> was highly expressed in petioles, which may be related to NO<sub>3</sub><sup>−</sup> storage in petioles; and <i>CsNPF2.8</i> was highly expressed in fruits, which may promote NO<sub>3</sub><sup>−</sup> transport to the embryos. We further examined their expression patterns under different abiotic stress and nitrogen conditions, and found that <i>CsNPF7.2</i> and <i>CsNPF7.3</i> responded to salt, cold, and low nitrogen stress. Taken together, our study lays a foundation for further exploration of the molecular and physiological functions of cucumber nitrate transporters. |
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| last_indexed | 2024-03-11T06:00:12Z |
| publishDate | 2023-03-01 |
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| spelling | doaj.art-620cfbb6c468442882dfdcf1910c9c1f2023-11-17T13:21:15ZengMDPI AGPlants2223-77472023-03-01126125210.3390/plants12061252Genome-Wide Identification and Expression Analysis of <i>NPF</i> Genes in Cucumber (<i>Cucumis sativus</i> L.)Mengying Zhang0Wenyan Zhang1Zijian Zheng2Zhiping Zhang3Bing Hua4Jiexia Liu5Minmin Miao6College of Horticulture and Landscape, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Landscape, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Landscape, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Landscape, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Landscape, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Landscape, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Landscape, Yangzhou University, Yangzhou 225009, ChinaThe NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER family (NPF) proteins perform an essential role in regulating plant nitrate absorption and distribution and in improving plant nitrogen use efficiency. In this study, cucumber (<i>Cucumis sativus</i> L.) <i>NPF</i> genes were comprehensively analyzed at the whole genome level, and 54 <i>NPF</i> genes were found to be unevenly distributed on seven chromosomes in the cucumber genome. The phylogenetic analysis showed that these genes could be divided into eight subfamilies. We renamed all <i>CsNPF</i> genes according to the international nomenclature, based on their homology with <i>AtNPF</i> genes. By surveying the expression profiles of <i>CsNPF</i> genes in various tissues, we found that <i>CsNPF6.4</i> was specifically expressed in roots, indicating that <i>CsNPF6.4</i> may play a role in N absorption; <i>CsNPF6.3</i> was highly expressed in petioles, which may be related to NO<sub>3</sub><sup>−</sup> storage in petioles; and <i>CsNPF2.8</i> was highly expressed in fruits, which may promote NO<sub>3</sub><sup>−</sup> transport to the embryos. We further examined their expression patterns under different abiotic stress and nitrogen conditions, and found that <i>CsNPF7.2</i> and <i>CsNPF7.3</i> responded to salt, cold, and low nitrogen stress. Taken together, our study lays a foundation for further exploration of the molecular and physiological functions of cucumber nitrate transporters.https://www.mdpi.com/2223-7747/12/6/1252cucumbernitrogen<i>NPF</i> genesabiotic stressnitrate transporter |
| spellingShingle | Mengying Zhang Wenyan Zhang Zijian Zheng Zhiping Zhang Bing Hua Jiexia Liu Minmin Miao Genome-Wide Identification and Expression Analysis of <i>NPF</i> Genes in Cucumber (<i>Cucumis sativus</i> L.) Plants cucumber nitrogen <i>NPF</i> genes abiotic stress nitrate transporter |
| title | Genome-Wide Identification and Expression Analysis of <i>NPF</i> Genes in Cucumber (<i>Cucumis sativus</i> L.) |
| title_full | Genome-Wide Identification and Expression Analysis of <i>NPF</i> Genes in Cucumber (<i>Cucumis sativus</i> L.) |
| title_fullStr | Genome-Wide Identification and Expression Analysis of <i>NPF</i> Genes in Cucumber (<i>Cucumis sativus</i> L.) |
| title_full_unstemmed | Genome-Wide Identification and Expression Analysis of <i>NPF</i> Genes in Cucumber (<i>Cucumis sativus</i> L.) |
| title_short | Genome-Wide Identification and Expression Analysis of <i>NPF</i> Genes in Cucumber (<i>Cucumis sativus</i> L.) |
| title_sort | genome wide identification and expression analysis of i npf i genes in cucumber i cucumis sativus i l |
| topic | cucumber nitrogen <i>NPF</i> genes abiotic stress nitrate transporter |
| url | https://www.mdpi.com/2223-7747/12/6/1252 |
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