Noninvasive Tracking of Hematopoietic Stem Cells in a Bone Marrow Transplant Model
The hematopoietic stem cell engraftment depends on adequate cell numbers, their homing, and the subsequent short and long-term engraftment of these cells in the niche. We performed a systematic review of the methods employed to track hematopoietic reconstitution using molecular imaging. We searched...
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MDPI AG
2020-04-01
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| Online Access: | https://www.mdpi.com/2073-4409/9/4/939 |
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| author | Fernando A. Oliveira Mariana P. Nucci Igor S. Filgueiras João M. Ferreira Leopoldo P. Nucci Javier B. Mamani Fernando Alvieri Lucas E. B. Souza Gabriel N. A. Rego Andrea T. Kondo Nelson Hamerschlak Lionel F. Gamarra |
| author_facet | Fernando A. Oliveira Mariana P. Nucci Igor S. Filgueiras João M. Ferreira Leopoldo P. Nucci Javier B. Mamani Fernando Alvieri Lucas E. B. Souza Gabriel N. A. Rego Andrea T. Kondo Nelson Hamerschlak Lionel F. Gamarra |
| author_sort | Fernando A. Oliveira |
| collection | DOAJ |
| description | The hematopoietic stem cell engraftment depends on adequate cell numbers, their homing, and the subsequent short and long-term engraftment of these cells in the niche. We performed a systematic review of the methods employed to track hematopoietic reconstitution using molecular imaging. We searched articles indexed, published prior to January 2020, in PubMed, Cochrane, and Scopus with the following keyword sequences: (Hematopoietic Stem Cell OR Hematopoietic Progenitor Cell) AND (Tracking OR Homing) AND (Transplantation). Of 2191 articles identified, only 21 articles were included in this review, after screening and eligibility assessment. The cell source was in the majority of bone marrow from mice (43%), followed by the umbilical cord from humans (33%). The labeling agent had the follow distribution between the selected studies: 14% nanoparticle, 29% radioisotope, 19% fluorophore, 19% luciferase, and 19% animal transgenic. The type of graft used in the studies was 57% allogeneic, 38% xenogeneic, and 5% autologous, being the HSC receptor: 57% mice, 9% rat, 19% fish, 5% for dog, porcine and salamander. The imaging technique used in the HSC tracking had the following distribution between studies: Positron emission tomography/single-photon emission computed tomography 29%, bioluminescence 33%, fluorescence 19%, magnetic resonance imaging 14%, and near-infrared fluorescence imaging 5%. The efficiency of the graft was evaluated in 61% of the selected studies, and before one month of implantation, the cell renewal was very low (less than 20%), but after three months, the efficiency was more than 50%, mainly in the allogeneic graft. In conclusion, our review showed an increase in using noninvasive imaging techniques in HSC tracking using the bone marrow transplant model. However, successful transplantation depends on the formation of engraftment, and the functionality of cells after the graft, aspects that are poorly explored and that have high relevance for clinical analysis. |
| first_indexed | 2024-03-10T20:32:55Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T20:32:55Z |
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| spelling | doaj.art-621262ceb8c245278f71b1d7bc8e93ce2023-11-19T21:16:59ZengMDPI AGCells2073-44092020-04-019493910.3390/cells9040939Noninvasive Tracking of Hematopoietic Stem Cells in a Bone Marrow Transplant ModelFernando A. Oliveira0Mariana P. Nucci1Igor S. Filgueiras2João M. Ferreira3Leopoldo P. Nucci4Javier B. Mamani5Fernando Alvieri6Lucas E. B. Souza7Gabriel N. A. Rego8Andrea T. Kondo9Nelson Hamerschlak10Lionel F. Gamarra11Hospital Israelita Albert Einstein, São Paulo 05652-900, BrazilLIM44—Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de São Paulo, São Paulo 01246-903, BrazilHospital Israelita Albert Einstein, São Paulo 05652-900, BrazilHospital Israelita Albert Einstein, São Paulo 05652-900, BrazilCentro Universitário do Planalto Central, Brasília DF 72445-020, BrazilHospital Israelita Albert Einstein, São Paulo 05652-900, BrazilHospital Israelita Albert Einstein, São Paulo 05652-900, BrazilFaculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto SP 14049-900, BrazilHospital Israelita Albert Einstein, São Paulo 05652-900, BrazilHospital Israelita Albert Einstein, São Paulo 05652-900, BrazilHospital Israelita Albert Einstein, São Paulo 05652-900, BrazilHospital Israelita Albert Einstein, São Paulo 05652-900, BrazilThe hematopoietic stem cell engraftment depends on adequate cell numbers, their homing, and the subsequent short and long-term engraftment of these cells in the niche. We performed a systematic review of the methods employed to track hematopoietic reconstitution using molecular imaging. We searched articles indexed, published prior to January 2020, in PubMed, Cochrane, and Scopus with the following keyword sequences: (Hematopoietic Stem Cell OR Hematopoietic Progenitor Cell) AND (Tracking OR Homing) AND (Transplantation). Of 2191 articles identified, only 21 articles were included in this review, after screening and eligibility assessment. The cell source was in the majority of bone marrow from mice (43%), followed by the umbilical cord from humans (33%). The labeling agent had the follow distribution between the selected studies: 14% nanoparticle, 29% radioisotope, 19% fluorophore, 19% luciferase, and 19% animal transgenic. The type of graft used in the studies was 57% allogeneic, 38% xenogeneic, and 5% autologous, being the HSC receptor: 57% mice, 9% rat, 19% fish, 5% for dog, porcine and salamander. The imaging technique used in the HSC tracking had the following distribution between studies: Positron emission tomography/single-photon emission computed tomography 29%, bioluminescence 33%, fluorescence 19%, magnetic resonance imaging 14%, and near-infrared fluorescence imaging 5%. The efficiency of the graft was evaluated in 61% of the selected studies, and before one month of implantation, the cell renewal was very low (less than 20%), but after three months, the efficiency was more than 50%, mainly in the allogeneic graft. In conclusion, our review showed an increase in using noninvasive imaging techniques in HSC tracking using the bone marrow transplant model. However, successful transplantation depends on the formation of engraftment, and the functionality of cells after the graft, aspects that are poorly explored and that have high relevance for clinical analysis.https://www.mdpi.com/2073-4409/9/4/939hematopoietic stem cellnanoparticlehomingtrackingnear-infrared fluorescence imagemagnetic resonance image |
| spellingShingle | Fernando A. Oliveira Mariana P. Nucci Igor S. Filgueiras João M. Ferreira Leopoldo P. Nucci Javier B. Mamani Fernando Alvieri Lucas E. B. Souza Gabriel N. A. Rego Andrea T. Kondo Nelson Hamerschlak Lionel F. Gamarra Noninvasive Tracking of Hematopoietic Stem Cells in a Bone Marrow Transplant Model Cells hematopoietic stem cell nanoparticle homing tracking near-infrared fluorescence image magnetic resonance image |
| title | Noninvasive Tracking of Hematopoietic Stem Cells in a Bone Marrow Transplant Model |
| title_full | Noninvasive Tracking of Hematopoietic Stem Cells in a Bone Marrow Transplant Model |
| title_fullStr | Noninvasive Tracking of Hematopoietic Stem Cells in a Bone Marrow Transplant Model |
| title_full_unstemmed | Noninvasive Tracking of Hematopoietic Stem Cells in a Bone Marrow Transplant Model |
| title_short | Noninvasive Tracking of Hematopoietic Stem Cells in a Bone Marrow Transplant Model |
| title_sort | noninvasive tracking of hematopoietic stem cells in a bone marrow transplant model |
| topic | hematopoietic stem cell nanoparticle homing tracking near-infrared fluorescence image magnetic resonance image |
| url | https://www.mdpi.com/2073-4409/9/4/939 |
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