Ultrasonic irrigation in the removal of smear layer and Enterococcus faecalis from root canals

Aim: This study evaluated both smear layer removal and reduction of Enterococcus faecalis after instrumentation with ultrasonic irrigation. Methods: Root canals were experimentally inoculated with E. faecalis for 20 days and microbiological samples were collected before and after chemomechanical pre...

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Main Authors: Letícia Maria Menezes Nóbrega, Cícero Romão Gadê-Neto, Fábio Roberto Dametto, Carlos Frederico de M. Sarmento, Rejane Andrade de Carvalho
Format: Article
Language:English
Published: Universidade Estadual de Campinas 2015-11-01
Series:Brazilian Journal of Oral Sciences
Subjects:
Online Access:https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8641637
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author Letícia Maria Menezes Nóbrega
Cícero Romão Gadê-Neto
Fábio Roberto Dametto
Carlos Frederico de M. Sarmento
Rejane Andrade de Carvalho
author_facet Letícia Maria Menezes Nóbrega
Cícero Romão Gadê-Neto
Fábio Roberto Dametto
Carlos Frederico de M. Sarmento
Rejane Andrade de Carvalho
author_sort Letícia Maria Menezes Nóbrega
collection DOAJ
description Aim: This study evaluated both smear layer removal and reduction of Enterococcus faecalis after instrumentation with ultrasonic irrigation. Methods: Root canals were experimentally inoculated with E. faecalis for 20 days and microbiological samples were collected before and after chemomechanical preparation by using sterilized absorbent paper points. The irrigation solutions used were NaOCl 2.5% and EDTA 17%. In Group 1 (G1), conventional irrigation was used, whereas in Group 2 (G2) ultrasonic irrigation was performed. In group 3 (control), root canals were irrigated with distilled water. The samples were inoculated in BHI broth and turbidity was observed after 48 h to evaluate the reduction in the number of bacteria. Residual smear layer was examined by scanning electron microscopy (SEM). Results: The results showed no significant differences between ultrasonic and conventional irrigation. Conclusions: It was concluded that the level of disinfection and cleanliness of root canals achieved with ultrasonic irrigation is comparable to that obtained by conventional methods.
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spelling doaj.art-62132006de604cb496d3e1396cf6b7222022-12-21T22:14:30ZengUniversidade Estadual de CampinasBrazilian Journal of Oral Sciences1677-32252015-11-0110310.20396/bjos.v10i3.8641637Ultrasonic irrigation in the removal of smear layer and Enterococcus faecalis from root canalsLetícia Maria Menezes Nóbrega0Cícero Romão Gadê-Neto1Fábio Roberto Dametto2Carlos Frederico de M. Sarmento3Rejane Andrade de Carvalho4Potiguar University (Laureate International Universities)Potiguar University (Laureate International Universities)Potiguar University (Laureate International Universities)Potiguar University (Laureate International Universities)Potiguar University (Laureate International Universities)Aim: This study evaluated both smear layer removal and reduction of Enterococcus faecalis after instrumentation with ultrasonic irrigation. Methods: Root canals were experimentally inoculated with E. faecalis for 20 days and microbiological samples were collected before and after chemomechanical preparation by using sterilized absorbent paper points. The irrigation solutions used were NaOCl 2.5% and EDTA 17%. In Group 1 (G1), conventional irrigation was used, whereas in Group 2 (G2) ultrasonic irrigation was performed. In group 3 (control), root canals were irrigated with distilled water. The samples were inoculated in BHI broth and turbidity was observed after 48 h to evaluate the reduction in the number of bacteria. Residual smear layer was examined by scanning electron microscopy (SEM). Results: The results showed no significant differences between ultrasonic and conventional irrigation. Conclusions: It was concluded that the level of disinfection and cleanliness of root canals achieved with ultrasonic irrigation is comparable to that obtained by conventional methods.https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8641637E. faecalisEndodontic irrigationSmear layerUltrasonic
spellingShingle Letícia Maria Menezes Nóbrega
Cícero Romão Gadê-Neto
Fábio Roberto Dametto
Carlos Frederico de M. Sarmento
Rejane Andrade de Carvalho
Ultrasonic irrigation in the removal of smear layer and Enterococcus faecalis from root canals
Brazilian Journal of Oral Sciences
E. faecalis
Endodontic irrigation
Smear layer
Ultrasonic
title Ultrasonic irrigation in the removal of smear layer and Enterococcus faecalis from root canals
title_full Ultrasonic irrigation in the removal of smear layer and Enterococcus faecalis from root canals
title_fullStr Ultrasonic irrigation in the removal of smear layer and Enterococcus faecalis from root canals
title_full_unstemmed Ultrasonic irrigation in the removal of smear layer and Enterococcus faecalis from root canals
title_short Ultrasonic irrigation in the removal of smear layer and Enterococcus faecalis from root canals
title_sort ultrasonic irrigation in the removal of smear layer and enterococcus faecalis from root canals
topic E. faecalis
Endodontic irrigation
Smear layer
Ultrasonic
url https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8641637
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AT carlosfredericodemsarmento ultrasonicirrigationintheremovalofsmearlayerandenterococcusfaecalisfromrootcanals
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