MICROPROPAGATION OF YUCCA PLANT BY USING GUAR AND LOCUST BEAN SEED POWDER AS AN ALTERNATIVE CHEAP GELLING AGENT
The present study aims to determine the best type and concentration of growth regulators and find cheap and/or readily-available alternative substitutes of agar for tissue culture media preparation. The results clear that MS medium supplemented with 1 mg l-1 BA significantly outperformed other teste...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Scientific Society for Flowers and Ornamental Plants
2020-09-01
|
| Series: | Scientific Journal of Flowers and Ornamental Plants |
| Subjects: | |
| Online Access: | https://sjfop.journals.ekb.eg/article_110364_33defbd16c902aaadc976197e3eddc57.pdf |
| _version_ | 1797833418193502208 |
|---|---|
| author | Hany Hassan M. Moubarak |
| author_facet | Hany Hassan M. Moubarak |
| author_sort | Hany Hassan |
| collection | DOAJ |
| description | The present study aims to determine the best type and concentration of growth regulators and find cheap and/or readily-available alternative substitutes of agar for tissue culture media preparation. The results clear that MS medium supplemented with 1 mg l-1 BA significantly outperformed other tested cytokinins concerning yucca shoot multiplication and growth parameters (number of shoots/explant, number of leaves/shoot, and shoot length (cm)) which recorded 4.75, 14.00 and 6.50 cm, respectively. Moreover, the combination between 2 g l-1 agar and 6 g l-1 guar as gelling agents produced the maximum shoot growth values. During rooting stage, data clear that 1.0 mg l-1 NAA increased number of leaves/shoot, number of roots/shoot and root length with mean values of 20.50, 3.75 and 5.22 cm, respectively. Finally, rooted plantlets were successfully acclimatized with maximum survivability percentage (100%) in soil mixture of sand and peat moss (1:1. v/v). |
| first_indexed | 2024-04-09T14:22:49Z |
| format | Article |
| id | doaj.art-621599f235b7445fb709f6abc2511cf8 |
| institution | Directory Open Access Journal |
| issn | 2356-7864 2536-9296 |
| language | English |
| last_indexed | 2024-04-09T14:22:49Z |
| publishDate | 2020-09-01 |
| publisher | Scientific Society for Flowers and Ornamental Plants |
| record_format | Article |
| series | Scientific Journal of Flowers and Ornamental Plants |
| spelling | doaj.art-621599f235b7445fb709f6abc2511cf82023-05-04T13:58:42ZengScientific Society for Flowers and Ornamental PlantsScientific Journal of Flowers and Ornamental Plants2356-78642536-92962020-09-017323924610.21608/sjfop.2020.110364110364MICROPROPAGATION OF YUCCA PLANT BY USING GUAR AND LOCUST BEAN SEED POWDER AS AN ALTERNATIVE CHEAP GELLING AGENTHany Hassan0M. Moubarak1Plant production department, Faculty of Environment Agricultural Sciences, Arish UniversityDepartment of Horticulture, Fac. Agric., Damanhour Univ., Damanhour City, EgyptThe present study aims to determine the best type and concentration of growth regulators and find cheap and/or readily-available alternative substitutes of agar for tissue culture media preparation. The results clear that MS medium supplemented with 1 mg l-1 BA significantly outperformed other tested cytokinins concerning yucca shoot multiplication and growth parameters (number of shoots/explant, number of leaves/shoot, and shoot length (cm)) which recorded 4.75, 14.00 and 6.50 cm, respectively. Moreover, the combination between 2 g l-1 agar and 6 g l-1 guar as gelling agents produced the maximum shoot growth values. During rooting stage, data clear that 1.0 mg l-1 NAA increased number of leaves/shoot, number of roots/shoot and root length with mean values of 20.50, 3.75 and 5.22 cm, respectively. Finally, rooted plantlets were successfully acclimatized with maximum survivability percentage (100%) in soil mixture of sand and peat moss (1:1. v/v).https://sjfop.journals.ekb.eg/article_110364_33defbd16c902aaadc976197e3eddc57.pdfyuccabagelling agentguar seeds powdernaa |
| spellingShingle | Hany Hassan M. Moubarak MICROPROPAGATION OF YUCCA PLANT BY USING GUAR AND LOCUST BEAN SEED POWDER AS AN ALTERNATIVE CHEAP GELLING AGENT Scientific Journal of Flowers and Ornamental Plants yucca ba gelling agent guar seeds powder naa |
| title | MICROPROPAGATION OF YUCCA PLANT BY USING GUAR AND LOCUST BEAN SEED POWDER AS AN ALTERNATIVE CHEAP GELLING AGENT |
| title_full | MICROPROPAGATION OF YUCCA PLANT BY USING GUAR AND LOCUST BEAN SEED POWDER AS AN ALTERNATIVE CHEAP GELLING AGENT |
| title_fullStr | MICROPROPAGATION OF YUCCA PLANT BY USING GUAR AND LOCUST BEAN SEED POWDER AS AN ALTERNATIVE CHEAP GELLING AGENT |
| title_full_unstemmed | MICROPROPAGATION OF YUCCA PLANT BY USING GUAR AND LOCUST BEAN SEED POWDER AS AN ALTERNATIVE CHEAP GELLING AGENT |
| title_short | MICROPROPAGATION OF YUCCA PLANT BY USING GUAR AND LOCUST BEAN SEED POWDER AS AN ALTERNATIVE CHEAP GELLING AGENT |
| title_sort | micropropagation of yucca plant by using guar and locust bean seed powder as an alternative cheap gelling agent |
| topic | yucca ba gelling agent guar seeds powder naa |
| url | https://sjfop.journals.ekb.eg/article_110364_33defbd16c902aaadc976197e3eddc57.pdf |
| work_keys_str_mv | AT hanyhassan micropropagationofyuccaplantbyusingguarandlocustbeanseedpowderasanalternativecheapgellingagent AT mmoubarak micropropagationofyuccaplantbyusingguarandlocustbeanseedpowderasanalternativecheapgellingagent |