Genetic diversity of Ethiopian durum wheat landraces.
Genetic diversity and population structure assessment in crops is essential for marker trait association, marker assisted breeding and crop germplasm conservation. We analyzed a set of 285 durum wheat accessions comprising 215 Ethiopian durum wheat landraces, 10 released durum wheat varieties, 10 ad...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2021-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0247016 |
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| author | Kefyalew Negisho Surafel Shibru Klaus Pillen Frank Ordon Gwendolin Wehner |
| author_facet | Kefyalew Negisho Surafel Shibru Klaus Pillen Frank Ordon Gwendolin Wehner |
| author_sort | Kefyalew Negisho |
| collection | DOAJ |
| description | Genetic diversity and population structure assessment in crops is essential for marker trait association, marker assisted breeding and crop germplasm conservation. We analyzed a set of 285 durum wheat accessions comprising 215 Ethiopian durum wheat landraces, 10 released durum wheat varieties, 10 advanced durum wheat lines from Ethiopia, and 50 durum wheat lines from CIMMYT. We investigated the genetic diversity and population structure for the complete panel as well as for the 215 landraces, separately based on 11,919 SNP markers with known physical positions. The whole panel was clustered into two populations representing on the one hand mainly the landraces, and on the other hand mainly released, advanced and CIMMYT lines. Further population structure analysis of the landraces uncovered 4 subgroups emphasizing the high degree of genetic diversity within Ethiopian durum landraces. Population structure based AMOVA for both sets unveiled significant (P < 0.001) variation between populations and within populations. Total variation within population accessions (81%, 76%) was higher than total variation between populations (19%, 24%) for both sets. Population structure analysis based genetic differentiation (FST) and gene flow (Nm) for the whole set and the Ethiopian landraces were 0.19 and 0.24, 1.04, and 0.81, respectively indicating high genetic differentiation and limited gene flow. Diversity indices verify that the landrace panel was more diverse with (I = 0.7, He = 0.46, uHe = 0.46) than the advanced lines (I = 0.6, He = 0.42, uHe = 0.42). Similarly, differences within the landrace clusters were observed. In summary a high genetic diversity within Ethiopian durum wheat landraces was detected, which may be a target for national and international wheat improvement programs to exploit valuable traits for biotic and abiotic stresses. |
| first_indexed | 2024-12-17T20:06:23Z |
| format | Article |
| id | doaj.art-62182d02bb4f4f79bae9d788ecb6a012 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-17T20:06:23Z |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-62182d02bb4f4f79bae9d788ecb6a0122022-12-21T21:34:19ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-01162e024701610.1371/journal.pone.0247016Genetic diversity of Ethiopian durum wheat landraces.Kefyalew NegishoSurafel ShibruKlaus PillenFrank OrdonGwendolin WehnerGenetic diversity and population structure assessment in crops is essential for marker trait association, marker assisted breeding and crop germplasm conservation. We analyzed a set of 285 durum wheat accessions comprising 215 Ethiopian durum wheat landraces, 10 released durum wheat varieties, 10 advanced durum wheat lines from Ethiopia, and 50 durum wheat lines from CIMMYT. We investigated the genetic diversity and population structure for the complete panel as well as for the 215 landraces, separately based on 11,919 SNP markers with known physical positions. The whole panel was clustered into two populations representing on the one hand mainly the landraces, and on the other hand mainly released, advanced and CIMMYT lines. Further population structure analysis of the landraces uncovered 4 subgroups emphasizing the high degree of genetic diversity within Ethiopian durum landraces. Population structure based AMOVA for both sets unveiled significant (P < 0.001) variation between populations and within populations. Total variation within population accessions (81%, 76%) was higher than total variation between populations (19%, 24%) for both sets. Population structure analysis based genetic differentiation (FST) and gene flow (Nm) for the whole set and the Ethiopian landraces were 0.19 and 0.24, 1.04, and 0.81, respectively indicating high genetic differentiation and limited gene flow. Diversity indices verify that the landrace panel was more diverse with (I = 0.7, He = 0.46, uHe = 0.46) than the advanced lines (I = 0.6, He = 0.42, uHe = 0.42). Similarly, differences within the landrace clusters were observed. In summary a high genetic diversity within Ethiopian durum wheat landraces was detected, which may be a target for national and international wheat improvement programs to exploit valuable traits for biotic and abiotic stresses.https://doi.org/10.1371/journal.pone.0247016 |
| spellingShingle | Kefyalew Negisho Surafel Shibru Klaus Pillen Frank Ordon Gwendolin Wehner Genetic diversity of Ethiopian durum wheat landraces. PLoS ONE |
| title | Genetic diversity of Ethiopian durum wheat landraces. |
| title_full | Genetic diversity of Ethiopian durum wheat landraces. |
| title_fullStr | Genetic diversity of Ethiopian durum wheat landraces. |
| title_full_unstemmed | Genetic diversity of Ethiopian durum wheat landraces. |
| title_short | Genetic diversity of Ethiopian durum wheat landraces. |
| title_sort | genetic diversity of ethiopian durum wheat landraces |
| url | https://doi.org/10.1371/journal.pone.0247016 |
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