Epitopes and Mimotopes Identification Using Phage Display for Vaccine Development against Infectious Pathogens
Traditional vaccines use inactivated or weakened forms of pathogens which could have side effects and inadequate immune responses. To overcome these challenges, phage display has emerged as a valuable tool for identifying specific epitopes that could be used in vaccines. This review emphasizes the d...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-06-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/11/7/1176 |
| _version_ | 1827731585157300224 |
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| author | Marco Palma |
| author_facet | Marco Palma |
| author_sort | Marco Palma |
| collection | DOAJ |
| description | Traditional vaccines use inactivated or weakened forms of pathogens which could have side effects and inadequate immune responses. To overcome these challenges, phage display has emerged as a valuable tool for identifying specific epitopes that could be used in vaccines. This review emphasizes the direct connection between epitope identification and vaccine development, filling a crucial gap in the field. This technique allows vaccines to be engineered to effectively stimulate the immune system by presenting carefully selected epitopes. Phage display involves screening libraries of random peptides or gene/genome fragments using serum samples from infected, convalescent, or vaccinated individuals. This method has been used to identify epitopes from various pathogens including SARS-CoV-2, Mycobacterium tuberculosis, hepatitis viruses, H5N1, HIV-1, Human T-lymphotropic virus 1, <i>Plasmodium falciparum</i>, <i>Trypanosoma cruzi</i>, and <i>Dirofilaria repens</i>. Bacteriophages offer advantages such as being immunogenic carriers, low production costs, and customization options, making them a promising alternative to traditional vaccines. The purpose of this study has been to highlight an approach that encompasses the entire process from epitope identification to vaccine production using a single technique, without requiring additional manipulation. Unlike conventional methods, phage display demonstrates exceptional efficiency and speed, which could provide significant advantages in critical scenarios such as pandemics. |
| first_indexed | 2024-03-11T00:35:11Z |
| format | Article |
| id | doaj.art-6218add138154e82919820c49ecd23a0 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-11T00:35:11Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-6218add138154e82919820c49ecd23a02023-11-18T21:40:42ZengMDPI AGVaccines2076-393X2023-06-01117117610.3390/vaccines11071176Epitopes and Mimotopes Identification Using Phage Display for Vaccine Development against Infectious PathogensMarco Palma0Institute for Globally Distributed Open Research and Education (IGDORE), 03181 Torrevieja, SpainTraditional vaccines use inactivated or weakened forms of pathogens which could have side effects and inadequate immune responses. To overcome these challenges, phage display has emerged as a valuable tool for identifying specific epitopes that could be used in vaccines. This review emphasizes the direct connection between epitope identification and vaccine development, filling a crucial gap in the field. This technique allows vaccines to be engineered to effectively stimulate the immune system by presenting carefully selected epitopes. Phage display involves screening libraries of random peptides or gene/genome fragments using serum samples from infected, convalescent, or vaccinated individuals. This method has been used to identify epitopes from various pathogens including SARS-CoV-2, Mycobacterium tuberculosis, hepatitis viruses, H5N1, HIV-1, Human T-lymphotropic virus 1, <i>Plasmodium falciparum</i>, <i>Trypanosoma cruzi</i>, and <i>Dirofilaria repens</i>. Bacteriophages offer advantages such as being immunogenic carriers, low production costs, and customization options, making them a promising alternative to traditional vaccines. The purpose of this study has been to highlight an approach that encompasses the entire process from epitope identification to vaccine production using a single technique, without requiring additional manipulation. Unlike conventional methods, phage display demonstrates exceptional efficiency and speed, which could provide significant advantages in critical scenarios such as pandemics.https://www.mdpi.com/2076-393X/11/7/1176epitopesmimotopesphage displayvaccinevaccine developmentpathogens |
| spellingShingle | Marco Palma Epitopes and Mimotopes Identification Using Phage Display for Vaccine Development against Infectious Pathogens Vaccines epitopes mimotopes phage display vaccine vaccine development pathogens |
| title | Epitopes and Mimotopes Identification Using Phage Display for Vaccine Development against Infectious Pathogens |
| title_full | Epitopes and Mimotopes Identification Using Phage Display for Vaccine Development against Infectious Pathogens |
| title_fullStr | Epitopes and Mimotopes Identification Using Phage Display for Vaccine Development against Infectious Pathogens |
| title_full_unstemmed | Epitopes and Mimotopes Identification Using Phage Display for Vaccine Development against Infectious Pathogens |
| title_short | Epitopes and Mimotopes Identification Using Phage Display for Vaccine Development against Infectious Pathogens |
| title_sort | epitopes and mimotopes identification using phage display for vaccine development against infectious pathogens |
| topic | epitopes mimotopes phage display vaccine vaccine development pathogens |
| url | https://www.mdpi.com/2076-393X/11/7/1176 |
| work_keys_str_mv | AT marcopalma epitopesandmimotopesidentificationusingphagedisplayforvaccinedevelopmentagainstinfectiouspathogens |