First Detection of <i>Francisella halioticida</i> Infecting a Wild Population of Blue Mussels <i>Mytilus edulis</i> in the United Kingdom
In the last decade, declines in the population of wild blue mussels <i>Mytilus edulis</i> in the Tamar estuary (United Kingdom) have been noted. In archived samples collected from 2013 to 2019, between 7% (in 2013) and 18% (in 2019) showed large granulocytoma and haemocytic infiltration...
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MDPI AG
2022-03-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/11/3/329 |
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| author | Irene Cano Abigail Parker Georgia M. Ward Matthew Green Stuart Ross John Bignell Caroline Daumich Rose Kerr Stephen W. Feist Frederico M. Batista |
| author_facet | Irene Cano Abigail Parker Georgia M. Ward Matthew Green Stuart Ross John Bignell Caroline Daumich Rose Kerr Stephen W. Feist Frederico M. Batista |
| author_sort | Irene Cano |
| collection | DOAJ |
| description | In the last decade, declines in the population of wild blue mussels <i>Mytilus edulis</i> in the Tamar estuary (United Kingdom) have been noted. In archived samples collected from 2013 to 2019, between 7% (in 2013) and 18% (in 2019) showed large granulocytoma and haemocytic infiltration in the interstitial tissue of the digestive gland. Four samples were selected for <i>16S rRNA</i> gene Nanopore sequencing. A consensus sequence of 1449 bp showed nucleotide similarities between 99.93–100% with published sequences of <i>Francisella halioticida</i>. In situ hybridisation (ISH) confirmed the presence of <i>F. halioticida</i> DNA within individual granulocytes of granulocytomas and also in prokaryotic-like inclusion bodies within the digestive epithelial cells. The design of diagnostic tests for surveillance of <i>F. halioticida,</i> including more specific ISH probes and sequencing the genome of the isolates infecting mussels, will shed more light on the pathogenicity and spread of this pathogen. |
| first_indexed | 2024-03-09T13:02:44Z |
| format | Article |
| id | doaj.art-621a7e11bafe43f58c3e3e49da6de158 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T13:02:44Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-621a7e11bafe43f58c3e3e49da6de1582023-11-30T21:52:32ZengMDPI AGPathogens2076-08172022-03-0111332910.3390/pathogens11030329First Detection of <i>Francisella halioticida</i> Infecting a Wild Population of Blue Mussels <i>Mytilus edulis</i> in the United KingdomIrene Cano0Abigail Parker1Georgia M. Ward2Matthew Green3Stuart Ross4John Bignell5Caroline Daumich6Rose Kerr7Stephen W. Feist8Frederico M. Batista9Cefas Weymouth Laboratory, International Centre of Excellence for Aquatic Animal Health, Barrack Road, Weymouth DT4 8UB, UKCefas Weymouth Laboratory, International Centre of Excellence for Aquatic Animal Health, Barrack Road, Weymouth DT4 8UB, UKCefas Weymouth Laboratory, International Centre of Excellence for Aquatic Animal Health, Barrack Road, Weymouth DT4 8UB, UKCefas Weymouth Laboratory, International Centre of Excellence for Aquatic Animal Health, Barrack Road, Weymouth DT4 8UB, UKCefas Weymouth Laboratory, International Centre of Excellence for Aquatic Animal Health, Barrack Road, Weymouth DT4 8UB, UKCefas Weymouth Laboratory, International Centre of Excellence for Aquatic Animal Health, Barrack Road, Weymouth DT4 8UB, UKCefas Weymouth Laboratory, International Centre of Excellence for Aquatic Animal Health, Barrack Road, Weymouth DT4 8UB, UKCefas Weymouth Laboratory, International Centre of Excellence for Aquatic Animal Health, Barrack Road, Weymouth DT4 8UB, UKCefas Weymouth Laboratory, International Centre of Excellence for Aquatic Animal Health, Barrack Road, Weymouth DT4 8UB, UKCefas Weymouth Laboratory, International Centre of Excellence for Aquatic Animal Health, Barrack Road, Weymouth DT4 8UB, UKIn the last decade, declines in the population of wild blue mussels <i>Mytilus edulis</i> in the Tamar estuary (United Kingdom) have been noted. In archived samples collected from 2013 to 2019, between 7% (in 2013) and 18% (in 2019) showed large granulocytoma and haemocytic infiltration in the interstitial tissue of the digestive gland. Four samples were selected for <i>16S rRNA</i> gene Nanopore sequencing. A consensus sequence of 1449 bp showed nucleotide similarities between 99.93–100% with published sequences of <i>Francisella halioticida</i>. In situ hybridisation (ISH) confirmed the presence of <i>F. halioticida</i> DNA within individual granulocytes of granulocytomas and also in prokaryotic-like inclusion bodies within the digestive epithelial cells. The design of diagnostic tests for surveillance of <i>F. halioticida,</i> including more specific ISH probes and sequencing the genome of the isolates infecting mussels, will shed more light on the pathogenicity and spread of this pathogen.https://www.mdpi.com/2076-0817/11/3/329<i>Francisella halioticida</i>blue mussels <i>Mytilus edulis</i>prokaryote cystgranulocytomaintracellular bacteriumNanopore sequencing |
| spellingShingle | Irene Cano Abigail Parker Georgia M. Ward Matthew Green Stuart Ross John Bignell Caroline Daumich Rose Kerr Stephen W. Feist Frederico M. Batista First Detection of <i>Francisella halioticida</i> Infecting a Wild Population of Blue Mussels <i>Mytilus edulis</i> in the United Kingdom Pathogens <i>Francisella halioticida</i> blue mussels <i>Mytilus edulis</i> prokaryote cyst granulocytoma intracellular bacterium Nanopore sequencing |
| title | First Detection of <i>Francisella halioticida</i> Infecting a Wild Population of Blue Mussels <i>Mytilus edulis</i> in the United Kingdom |
| title_full | First Detection of <i>Francisella halioticida</i> Infecting a Wild Population of Blue Mussels <i>Mytilus edulis</i> in the United Kingdom |
| title_fullStr | First Detection of <i>Francisella halioticida</i> Infecting a Wild Population of Blue Mussels <i>Mytilus edulis</i> in the United Kingdom |
| title_full_unstemmed | First Detection of <i>Francisella halioticida</i> Infecting a Wild Population of Blue Mussels <i>Mytilus edulis</i> in the United Kingdom |
| title_short | First Detection of <i>Francisella halioticida</i> Infecting a Wild Population of Blue Mussels <i>Mytilus edulis</i> in the United Kingdom |
| title_sort | first detection of i francisella halioticida i infecting a wild population of blue mussels i mytilus edulis i in the united kingdom |
| topic | <i>Francisella halioticida</i> blue mussels <i>Mytilus edulis</i> prokaryote cyst granulocytoma intracellular bacterium Nanopore sequencing |
| url | https://www.mdpi.com/2076-0817/11/3/329 |
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