Development of a Smart Fluorescent Probe Specifically Interacting with C-Myc I-Motif
I-motifs play key regulatory roles in biological processes, holding great potential as attractive therapeutic targets. In the present study, we developed a novel fluorescent probe <b>G59</b> with strong and selective binding to the <i>c-myc</i> gene promoter i-motif. <b>...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-03-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/7/3872 |
| Summary: | I-motifs play key regulatory roles in biological processes, holding great potential as attractive therapeutic targets. In the present study, we developed a novel fluorescent probe <b>G59</b> with strong and selective binding to the <i>c-myc</i> gene promoter i-motif. <b>G59</b> had an i-motif-binding carbazole moiety conjugated with naphthalimide fluorescent groups. <b>G59</b> could differentiate the <i>c-myc</i> i-motif from other DNA structures through selective activation of its fluorescence, with its apparent visualization in solution. The smart probe <b>G59</b> showed excellent sensitivity, with a low fluorescent detection limit of 154 nM and effective stabilization to the <i>c-myc</i> i-motif. <b>G59</b> could serve as a rapid and sensitive probe for label-free screening of selective <i>c-myc</i> i-motif binding ligands under neutral crowding conditions. To the best of our knowledge, <b>G59</b> is the first fluorescent probe with high sensitivity for recognizing the i-motif structure and screening for selective binding ligands. |
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| ISSN: | 1661-6596 1422-0067 |