Variation for N uptake system in maize: genotypic response to N supply
An understanding of the adaptations made by plants in their nitrogen (N) uptake systems in response to reduced N supply is important to the development of cereals with enhanced N uptake efficiency (NUpE). Twenty seven diverse genotypes of maize (Zea mays, L.) were grown in hydroponics for three week...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2015-11-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00936/full |
| _version_ | 1818083988248461312 |
|---|---|
| author | Trevor eGarnett Trevor eGarnett Darren ePlett Darren ePlett Vanessa eConn Vanessa eConn Simon eConn Simon eConn Huwaida eRabie Antoni eRafalksi Kanwarpal eDhugga Mark eTester Mark eTester Brent eKaiser |
| author_facet | Trevor eGarnett Trevor eGarnett Darren ePlett Darren ePlett Vanessa eConn Vanessa eConn Simon eConn Simon eConn Huwaida eRabie Antoni eRafalksi Kanwarpal eDhugga Mark eTester Mark eTester Brent eKaiser |
| author_sort | Trevor eGarnett |
| collection | DOAJ |
| description | An understanding of the adaptations made by plants in their nitrogen (N) uptake systems in response to reduced N supply is important to the development of cereals with enhanced N uptake efficiency (NUpE). Twenty seven diverse genotypes of maize (Zea mays, L.) were grown in hydroponics for three weeks with limiting or adequate N supply. Genotype response to N was assessed on the basis of biomass characteristics and the activities of the nitrate (NO3-) and ammonium (NH4+) high-affinity transport systems. Genotypes differed greatly for the ability to maintain biomass with reduced N. Genotypes that maintained biomass at reduced N maintained net N uptake with no change in size of the root relative to the shoot. The root uptake capacity for both NO3- and NH4+ increased with reduced N. Transcript levels of putative NO3- and NH4+ transporter genes in the root tissue of a subset of the genotypes revealed that is was predominately putative ZmNRT2s whose transcript levels responded to N treatments. The correlation between the ratio of transcripts of ZmNRT2.2 between the two N levels and a genotypes ability to maintain biomass with reduced N suggests a role in enhancing N uptake efficiency. The observed variation in the ability to capture N at low N provides scope for both improving NUpE in maize and also to better understand the N uptake system in cereals. |
| first_indexed | 2024-12-10T19:46:44Z |
| format | Article |
| id | doaj.art-621dab32d6754f35baef72eac456a26f |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-10T19:46:44Z |
| publishDate | 2015-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-621dab32d6754f35baef72eac456a26f2022-12-22T01:35:52ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2015-11-01610.3389/fpls.2015.00936160512Variation for N uptake system in maize: genotypic response to N supplyTrevor eGarnett0Trevor eGarnett1Darren ePlett2Darren ePlett3Vanessa eConn4Vanessa eConn5Simon eConn6Simon eConn7Huwaida eRabie8Antoni eRafalksi9Kanwarpal eDhugga10Mark eTester11Mark eTester12Brent eKaiser13University of AdelaideThe University of AdelaideUniversity of AdelaideThe University of AdelaideUniversity of AdelaideThe University of AdelaideUniversity of AdelaideThe University of AdelaideBethlehem UniversityDuPont Crop GeneticsDuPont PioneerUniversity of AdelaideThe University of AdelaideUniversity of AdelaideAn understanding of the adaptations made by plants in their nitrogen (N) uptake systems in response to reduced N supply is important to the development of cereals with enhanced N uptake efficiency (NUpE). Twenty seven diverse genotypes of maize (Zea mays, L.) were grown in hydroponics for three weeks with limiting or adequate N supply. Genotype response to N was assessed on the basis of biomass characteristics and the activities of the nitrate (NO3-) and ammonium (NH4+) high-affinity transport systems. Genotypes differed greatly for the ability to maintain biomass with reduced N. Genotypes that maintained biomass at reduced N maintained net N uptake with no change in size of the root relative to the shoot. The root uptake capacity for both NO3- and NH4+ increased with reduced N. Transcript levels of putative NO3- and NH4+ transporter genes in the root tissue of a subset of the genotypes revealed that is was predominately putative ZmNRT2s whose transcript levels responded to N treatments. The correlation between the ratio of transcripts of ZmNRT2.2 between the two N levels and a genotypes ability to maintain biomass with reduced N suggests a role in enhancing N uptake efficiency. The observed variation in the ability to capture N at low N provides scope for both improving NUpE in maize and also to better understand the N uptake system in cereals.http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00936/fullNitrogenZea maysMaizetransportersuptakeammonium |
| spellingShingle | Trevor eGarnett Trevor eGarnett Darren ePlett Darren ePlett Vanessa eConn Vanessa eConn Simon eConn Simon eConn Huwaida eRabie Antoni eRafalksi Kanwarpal eDhugga Mark eTester Mark eTester Brent eKaiser Variation for N uptake system in maize: genotypic response to N supply Frontiers in Plant Science Nitrogen Zea mays Maize transporters uptake ammonium |
| title | Variation for N uptake system in maize: genotypic response to N supply |
| title_full | Variation for N uptake system in maize: genotypic response to N supply |
| title_fullStr | Variation for N uptake system in maize: genotypic response to N supply |
| title_full_unstemmed | Variation for N uptake system in maize: genotypic response to N supply |
| title_short | Variation for N uptake system in maize: genotypic response to N supply |
| title_sort | variation for n uptake system in maize genotypic response to n supply |
| topic | Nitrogen Zea mays Maize transporters uptake ammonium |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00936/full |
| work_keys_str_mv | AT trevoregarnett variationfornuptakesysteminmaizegenotypicresponsetonsupply AT trevoregarnett variationfornuptakesysteminmaizegenotypicresponsetonsupply AT darreneplett variationfornuptakesysteminmaizegenotypicresponsetonsupply AT darreneplett variationfornuptakesysteminmaizegenotypicresponsetonsupply AT vanessaeconn variationfornuptakesysteminmaizegenotypicresponsetonsupply AT vanessaeconn variationfornuptakesysteminmaizegenotypicresponsetonsupply AT simoneconn variationfornuptakesysteminmaizegenotypicresponsetonsupply AT simoneconn variationfornuptakesysteminmaizegenotypicresponsetonsupply AT huwaidaerabie variationfornuptakesysteminmaizegenotypicresponsetonsupply AT antonierafalksi variationfornuptakesysteminmaizegenotypicresponsetonsupply AT kanwarpaledhugga variationfornuptakesysteminmaizegenotypicresponsetonsupply AT marketester variationfornuptakesysteminmaizegenotypicresponsetonsupply AT marketester variationfornuptakesysteminmaizegenotypicresponsetonsupply AT brentekaiser variationfornuptakesysteminmaizegenotypicresponsetonsupply |