Virucidal effect of povidone-iodine against SARS-CoV-2
Objective To evaluate the antiviral activity of the oral disinfectant povidone-iodine (PVP-I) against severe acute respiratory syndrome-coronavirus-2 (SARS-CoV2) in vitro . Methods The cytotoxic effects of PVP-I were determined in Vero and Calu-3 cell lines using that by Cell Counting Kit-8 assay. V...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2021-12-01
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| Series: | Journal of International Medical Research |
| Online Access: | https://doi.org/10.1177/03000605211063695 |
| _version_ | 1828139287841865728 |
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| author | Ying Wang Yutong Wu Qingjing Wang Jiajie Zhu Wen Shi Zetao Han Yanjun Zhang Keda Chen |
| author_facet | Ying Wang Yutong Wu Qingjing Wang Jiajie Zhu Wen Shi Zetao Han Yanjun Zhang Keda Chen |
| author_sort | Ying Wang |
| collection | DOAJ |
| description | Objective To evaluate the antiviral activity of the oral disinfectant povidone-iodine (PVP-I) against severe acute respiratory syndrome-coronavirus-2 (SARS-CoV2) in vitro . Methods The cytotoxic effects of PVP-I were determined in Vero and Calu-3 cell lines using that by Cell Counting Kit-8 assay. Viral load in the cell culture medium above infected cells was quantitated using real-time polymerase chain reaction. The cytopathic effect (CPE) and viral infective rate were observed by immunofluorescence microscopy. Results PVP-I at a concentration >0.5 mg/ml in contact with SARS-CoV-2 for 30 s, 1 min, 2 min and 5 min showed up to 99% viral inhibition. For in vitro testing, upon exposure for 1 min, PVP-I showed a virucidal effect. PVP-I had no cytotoxic effects at the range of concentrations tested (0.125–1 mg/ml; CC 50 > 2.75 mM) in Vero and Calu-3 cells. Conclusion These results demonstrate that the ideal contact time was 1 min and the optimal concentration was 1 mg/ml, which provides an experimental basis for the use of oral disinfectants in dental hospitals. |
| first_indexed | 2024-04-11T18:49:36Z |
| format | Article |
| id | doaj.art-621e32fdc0154d029136b25cac8959d3 |
| institution | Directory Open Access Journal |
| issn | 1473-2300 |
| language | English |
| last_indexed | 2024-04-11T18:49:36Z |
| publishDate | 2021-12-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of International Medical Research |
| spelling | doaj.art-621e32fdc0154d029136b25cac8959d32022-12-22T04:08:28ZengSAGE PublishingJournal of International Medical Research1473-23002021-12-014910.1177/03000605211063695Virucidal effect of povidone-iodine against SARS-CoV-2Ying WangYutong WuQingjing WangJiajie ZhuWen ShiZetao HanYanjun ZhangKeda ChenObjective To evaluate the antiviral activity of the oral disinfectant povidone-iodine (PVP-I) against severe acute respiratory syndrome-coronavirus-2 (SARS-CoV2) in vitro . Methods The cytotoxic effects of PVP-I were determined in Vero and Calu-3 cell lines using that by Cell Counting Kit-8 assay. Viral load in the cell culture medium above infected cells was quantitated using real-time polymerase chain reaction. The cytopathic effect (CPE) and viral infective rate were observed by immunofluorescence microscopy. Results PVP-I at a concentration >0.5 mg/ml in contact with SARS-CoV-2 for 30 s, 1 min, 2 min and 5 min showed up to 99% viral inhibition. For in vitro testing, upon exposure for 1 min, PVP-I showed a virucidal effect. PVP-I had no cytotoxic effects at the range of concentrations tested (0.125–1 mg/ml; CC 50 > 2.75 mM) in Vero and Calu-3 cells. Conclusion These results demonstrate that the ideal contact time was 1 min and the optimal concentration was 1 mg/ml, which provides an experimental basis for the use of oral disinfectants in dental hospitals.https://doi.org/10.1177/03000605211063695 |
| spellingShingle | Ying Wang Yutong Wu Qingjing Wang Jiajie Zhu Wen Shi Zetao Han Yanjun Zhang Keda Chen Virucidal effect of povidone-iodine against SARS-CoV-2 Journal of International Medical Research |
| title | Virucidal effect of povidone-iodine against SARS-CoV-2 |
| title_full | Virucidal effect of povidone-iodine against SARS-CoV-2 |
| title_fullStr | Virucidal effect of povidone-iodine against SARS-CoV-2 |
| title_full_unstemmed | Virucidal effect of povidone-iodine against SARS-CoV-2 |
| title_short | Virucidal effect of povidone-iodine against SARS-CoV-2 |
| title_sort | virucidal effect of povidone iodine against sars cov 2 |
| url | https://doi.org/10.1177/03000605211063695 |
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