Comparison of Fluorescence Flow Cytometry and Culture Method for Identification of Urine Specimens with No Growth
Introduction: In clinical microbiology laboratories, urine specimens are the most frequently analysed specimens. Rapid and reliable methods are needed especially for no growth culture. In this study, the performance of the Sysmex UF-1000i instrument was compared with the culture results for the purp...
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| Format: | Article |
| Language: | English |
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Bilimsel Tip Yayinevi
2019-12-01
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| Series: | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi |
| Subjects: | |
| Online Access: | http://www.floradergisi.org/getFileContent.aspx?op=REDPDF&file_name=2019-24-04-321-328.pdf |
| _version_ | 1797916154496286720 |
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| author | Şölen DALDABAN DİNÇER Özgür YANILMAZ Ülkü ORAL ZEYTİNLİ Ertan ÖZYURT Ramazan AYAŞ Sebahat AKSARAY |
| author_facet | Şölen DALDABAN DİNÇER Özgür YANILMAZ Ülkü ORAL ZEYTİNLİ Ertan ÖZYURT Ramazan AYAŞ Sebahat AKSARAY |
| author_sort | Şölen DALDABAN DİNÇER |
| collection | DOAJ |
| description | Introduction: In clinical microbiology laboratories, urine specimens are the most frequently analysed specimens. Rapid and reliable methods are needed especially for no growth culture. In this study, the performance of the Sysmex UF-1000i instrument was compared with the culture results for the purpose of fast and reliable evaluation of the urine specimens.
Materials and Methods: 9525 urine samples sent for urine culture to the Central Laboratory between February 2018-April 2018 were evaluated retrospectively. The culture results and the leukocyte and bacterial results obtained from the fluorescence flow cytometer were matched and statistical analysis was performed by applying SPSS 15.00. ROC curves were plotted to determine the cut-off in 100% negative predictive value and 100% sensitivity to be used for leukocyte and bacterial screening.
Results: 9.6% of the urine specimens were reported to have shown growth, 33.4% were contaminated, and 57% did not show growth. The cut off value with 100% negative predictive value and the highest elimination rate in male and pediatric patients in this study were as follows: bacteria < 50/μL, leukocytes 15/μL, and in female patients as < 50/μL of bacteria and 10/μL. It was determined that 63.63% of the cases in male patients, 52.54% in pediatric patients and 19.06% in female patients did not require a culture study.
Conclusion: The flow cytometry system allows pre-culture screening to be accomplished in significant quantities without being subjected to culture. It will help prevent resistance development by avoiding the use of unnecessary antibiotics. |
| first_indexed | 2024-04-10T12:52:43Z |
| format | Article |
| id | doaj.art-621e4021890e4dd298a12fd1a3786a33 |
| institution | Directory Open Access Journal |
| issn | 1300-932X 1300-932X |
| language | English |
| last_indexed | 2024-04-10T12:52:43Z |
| publishDate | 2019-12-01 |
| publisher | Bilimsel Tip Yayinevi |
| record_format | Article |
| series | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi |
| spelling | doaj.art-621e4021890e4dd298a12fd1a3786a332023-02-15T16:13:39ZengBilimsel Tip YayineviFlora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi1300-932X1300-932X2019-12-0124432132810.5578/flora.68138Comparison of Fluorescence Flow Cytometry and Culture Method for Identification of Urine Specimens with No GrowthŞölen DALDABAN DİNÇER0Özgür YANILMAZ1Ülkü ORAL ZEYTİNLİ2Ertan ÖZYURT3Ramazan AYAŞ4Sebahat AKSARAY5Department of Microbiology, Istanbul Public Hospitals Services-2 Central Laboratory, Istanbul, TurkeyDepartment of Microbiology, Istanbul Public Hospitals Services-2 Central Laboratory, Istanbul, TurkeyDepartment of Microbiology, Istanbul Public Hospitals Services-2 Central Laboratory, Istanbul, TurkeyDepartment of Microbiology, Istanbul Public Hospitals Services-2 Central Laboratory, Istanbul, TurkeySysmex Diagnostic Turkey, Istanbul, TurkeyDepartment of Microbiology, Istanbul Public Hospitals Services-2 Central Laboratory, Istanbul, TurkeyIntroduction: In clinical microbiology laboratories, urine specimens are the most frequently analysed specimens. Rapid and reliable methods are needed especially for no growth culture. In this study, the performance of the Sysmex UF-1000i instrument was compared with the culture results for the purpose of fast and reliable evaluation of the urine specimens. Materials and Methods: 9525 urine samples sent for urine culture to the Central Laboratory between February 2018-April 2018 were evaluated retrospectively. The culture results and the leukocyte and bacterial results obtained from the fluorescence flow cytometer were matched and statistical analysis was performed by applying SPSS 15.00. ROC curves were plotted to determine the cut-off in 100% negative predictive value and 100% sensitivity to be used for leukocyte and bacterial screening. Results: 9.6% of the urine specimens were reported to have shown growth, 33.4% were contaminated, and 57% did not show growth. The cut off value with 100% negative predictive value and the highest elimination rate in male and pediatric patients in this study were as follows: bacteria < 50/μL, leukocytes 15/μL, and in female patients as < 50/μL of bacteria and 10/μL. It was determined that 63.63% of the cases in male patients, 52.54% in pediatric patients and 19.06% in female patients did not require a culture study. Conclusion: The flow cytometry system allows pre-culture screening to be accomplished in significant quantities without being subjected to culture. It will help prevent resistance development by avoiding the use of unnecessary antibiotics.http://www.floradergisi.org/getFileContent.aspx?op=REDPDF&file_name=2019-24-04-321-328.pdffluorescence flow cytometryurine culturenegative predictive value |
| spellingShingle | Şölen DALDABAN DİNÇER Özgür YANILMAZ Ülkü ORAL ZEYTİNLİ Ertan ÖZYURT Ramazan AYAŞ Sebahat AKSARAY Comparison of Fluorescence Flow Cytometry and Culture Method for Identification of Urine Specimens with No Growth Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi fluorescence flow cytometry urine culture negative predictive value |
| title | Comparison of Fluorescence Flow Cytometry and Culture Method for Identification of Urine Specimens with No Growth |
| title_full | Comparison of Fluorescence Flow Cytometry and Culture Method for Identification of Urine Specimens with No Growth |
| title_fullStr | Comparison of Fluorescence Flow Cytometry and Culture Method for Identification of Urine Specimens with No Growth |
| title_full_unstemmed | Comparison of Fluorescence Flow Cytometry and Culture Method for Identification of Urine Specimens with No Growth |
| title_short | Comparison of Fluorescence Flow Cytometry and Culture Method for Identification of Urine Specimens with No Growth |
| title_sort | comparison of fluorescence flow cytometry and culture method for identification of urine specimens with no growth |
| topic | fluorescence flow cytometry urine culture negative predictive value |
| url | http://www.floradergisi.org/getFileContent.aspx?op=REDPDF&file_name=2019-24-04-321-328.pdf |
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