Applying Principles of Multi-Factor Genetic Analysis of Infectious Disease Agents in the Work of the Rospotrebnadzor SAET during Mass Events
Objective of the study – to develop and test in operation of the Rospotrebnadzor Spesialized Anti-Epidemic Teams (SAET), deployed during the major mass events, the procedure for application of molecular-genetic methods for detection and identification of pathogenic biological agents. Materials and m...
| Main Authors: | , , |
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| Format: | Article |
| Language: | Russian |
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Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”
2018-07-01
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| Series: | Проблемы особо опасных инфекций |
| Subjects: | |
| Online Access: | https://journal.microbe.ru/jour/article/view/482 |
| _version_ | 1797220800463372288 |
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| author | I. V. Kuznetsova D. V. Efremenko A. N. Kulichenko |
| author_facet | I. V. Kuznetsova D. V. Efremenko A. N. Kulichenko |
| author_sort | I. V. Kuznetsova |
| collection | DOAJ |
| description | Objective of the study – to develop and test in operation of the Rospotrebnadzor Spesialized Anti-Epidemic Teams (SAET), deployed during the major mass events, the procedure for application of molecular-genetic methods for detection and identification of pathogenic biological agents. Materials and methods. Utilized are the reporting documentation drawn up by the results of work of the specialized anti-epidemic teams of the Rospotrebnadzor on the provision of sanitary epidemiological welfare during Olympic Games 2014 and other important mass events in Sochi in 2015–2017. Results and conclusions. Developed and tested in practice of the Rospotrebnadzor SAET during the major mass events has been procedure for application of molecular-genetic methods, based on structured approach (algorithm) of indication and genetic characterization of PBA. Equipping of the SAET with diagnostic preparations is carried out in accordance with the provision of preparedness for performing investigations by three-level algorithm of molecular-genetic characteristics of pathogens: level I – detection (indication) of a pathogen using PCR, level II – identification of the pathogen genome fragments using PCR, level III – genotyping. Developed procedure was used successfully by the SAET during the Olympic Games 2014 and other important mass events in Sochi in 2015–2017, which allows for recommending it for future use. |
| first_indexed | 2024-03-08T13:42:18Z |
| format | Article |
| id | doaj.art-6221f0d592f248968fcbb10d4fca7bb2 |
| institution | Directory Open Access Journal |
| issn | 0370-1069 2658-719X |
| language | Russian |
| last_indexed | 2024-04-24T12:55:18Z |
| publishDate | 2018-07-01 |
| publisher | Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” |
| record_format | Article |
| series | Проблемы особо опасных инфекций |
| spelling | doaj.art-6221f0d592f248968fcbb10d4fca7bb22024-04-05T16:47:13ZrusFederal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”Проблемы особо опасных инфекций0370-10692658-719X2018-07-0102687210.21055/0370-1069-2018-2-68-72471Applying Principles of Multi-Factor Genetic Analysis of Infectious Disease Agents in the Work of the Rospotrebnadzor SAET during Mass EventsI. V. Kuznetsova0D. V. Efremenko1A. N. Kulichenko2Stavropol Research Anti-Plague Institute, StavropolStavropol Research Anti-Plague Institute, StavropolStavropol Research Anti-Plague Institute, StavropolObjective of the study – to develop and test in operation of the Rospotrebnadzor Spesialized Anti-Epidemic Teams (SAET), deployed during the major mass events, the procedure for application of molecular-genetic methods for detection and identification of pathogenic biological agents. Materials and methods. Utilized are the reporting documentation drawn up by the results of work of the specialized anti-epidemic teams of the Rospotrebnadzor on the provision of sanitary epidemiological welfare during Olympic Games 2014 and other important mass events in Sochi in 2015–2017. Results and conclusions. Developed and tested in practice of the Rospotrebnadzor SAET during the major mass events has been procedure for application of molecular-genetic methods, based on structured approach (algorithm) of indication and genetic characterization of PBA. Equipping of the SAET with diagnostic preparations is carried out in accordance with the provision of preparedness for performing investigations by three-level algorithm of molecular-genetic characteristics of pathogens: level I – detection (indication) of a pathogen using PCR, level II – identification of the pathogen genome fragments using PCR, level III – genotyping. Developed procedure was used successfully by the SAET during the Olympic Games 2014 and other important mass events in Sochi in 2015–2017, which allows for recommending it for future use.https://journal.microbe.ru/jour/article/view/482pathogenic biological agentsmethods of genetic analysismass eventssaet |
| spellingShingle | I. V. Kuznetsova D. V. Efremenko A. N. Kulichenko Applying Principles of Multi-Factor Genetic Analysis of Infectious Disease Agents in the Work of the Rospotrebnadzor SAET during Mass Events Проблемы особо опасных инфекций pathogenic biological agents methods of genetic analysis mass events saet |
| title | Applying Principles of Multi-Factor Genetic Analysis of Infectious Disease Agents in the Work of the Rospotrebnadzor SAET during Mass Events |
| title_full | Applying Principles of Multi-Factor Genetic Analysis of Infectious Disease Agents in the Work of the Rospotrebnadzor SAET during Mass Events |
| title_fullStr | Applying Principles of Multi-Factor Genetic Analysis of Infectious Disease Agents in the Work of the Rospotrebnadzor SAET during Mass Events |
| title_full_unstemmed | Applying Principles of Multi-Factor Genetic Analysis of Infectious Disease Agents in the Work of the Rospotrebnadzor SAET during Mass Events |
| title_short | Applying Principles of Multi-Factor Genetic Analysis of Infectious Disease Agents in the Work of the Rospotrebnadzor SAET during Mass Events |
| title_sort | applying principles of multi factor genetic analysis of infectious disease agents in the work of the rospotrebnadzor saet during mass events |
| topic | pathogenic biological agents methods of genetic analysis mass events saet |
| url | https://journal.microbe.ru/jour/article/view/482 |
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